Detection of Human Bocavirus in Egyptian Children Suffering from Acute Lower Respiratory Tract Infection

Atef, Dina M.; Elbehedy, Rabab Mohamed

doi:10.20546/ijcmas.2017.602.090

Cited by 5 publications

(9 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This study has shown that 4 cases (50%) of HBoV-positive children were suffering from diarrhea which is comparable with other studies such as Lasure andGopalkrishna, 2017 andAmr et al, 2017; they found that 47% and 38% respectively. Otherwise, about 15% of Saudi children had diarrhea with HBoV-positive in RTI (Abdel-Moneim et al, 2013).…”

Section: Fig 2: Gel Electrophoresis Of the First Round Pcr With Prodsupporting

confidence: 90%

Detection of Human Bocavirus Infection in Children with Lower Respiratory

Al-Mayah¹,

Hussein²,

Hasan³

2018

RJS

View full text Add to dashboard Cite

Background: Respiratory tract diseases are one of the leading causes of morbidity and mortality in young children, and a great variety of viruses are responsible of these infection. Objectives: To determine the infection rate of human bocavirus in children with lower respiratory tract infection and related with different variable. Patients and methods: Cross sectional study consists of 122 children under five years old their age ranged between 1 and 60 months and 75 males and 47 females; suffering from lower respiratory tract infection. They were attending respiratory wards in Fatima Al

show abstract

Section: Fig 2: Gel Electrophoresis Of the First Round Pcr With Prodsupporting

confidence: 90%

Detection of Human Bocavirus Infection in Children with Lower Respiratory

Al-Mayah¹,

Hussein²,

Hasan³

2018

RJS

View full text Add to dashboard Cite

show abstract

“…Higher seroprevalences (13,18 and 13.2 %) were reported by Zaghloul, Amr et al . and Xu et al ., respectively, in children with ARIs [38, 40, 43]. Although a higher ELISA sensitivity (97 %) was reported by Lindner et al .…”

Section: Discussionmentioning

confidence: 99%

“…at Abo-Alreesh Pediatric Hospital during the period 2013–2014 [39] and Amr et al . at the Hospital of Zagazig University during the period 2015–2016, respectively [40]. On the other hand, lower incidences were reported by Rasheed et al .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Detection and phylogenetic analysis of Human bocavirus in children diagnosed with acute respiratory tract infection

Abozahra

Abdelhamid

Khairy

et al. 2020

Journal of Medical Microbiology

View full text Add to dashboard Cite

Introduction. Human bocavirus (HBoV) is a recently discovered parvovirus; it has been shown to be a common cause of respiratory infections and gastroenteritis in children. Since its identification, HBoV has been detected worldwide in nasopharyngeal swabs, serum and stool samples particularly those obtained from young children suffering from respiratory or gastrointestinal tract infections. Aim. The aim of this work was to determine HBoV prevalence among children with acute respiratory tract infection in Egypt, to detect the most prevalent HBoV genotype and to compare PCR and ELISA as diagnostic techniques for HBoV infection. Methods. Nasopharyngeal swabs and blood samples were obtained within the first day of admission from 75 children diagnosed with acute respiratory tract infection in El-Shatby University Hospital for Children in Alexandria, Egypt from October 2018 to March 2019. Conventional PCR was used to detect HBoV DNA, ELISA was used to detect HBoV IgM antibodies and sequencing of the VP1/2 genes was used for genotyping. Results. Seven (9.3%) of the 75 nasopharyngeal swabs obtained from patients with acute respiratory tract infection were positive for HBoV by PCR, while 5 (6.7 %) of the 75 serum samples were positive for HBoV IgM antibodies using ELISA. The correlation between PCR and ELISA results showed a highly significant association between PCR and ELISA techniques (X 2=52.041, P<0.01) and a highly significant agreement between the two methods (Kappa=81.9 %, P<0.01). Phylogenetic analysis showed that all positive samples were related to the HBoV-1 genotype. Conclusion. Human bocavirus was detected at 9.3 % prevalence in nasopharyngeal swabs obtained from children with acute respiratory tract infection. The HBoV-1 genotype was the only genotype detected, suggesting that a single genetic lineage of HBoV is circulating in Egypt. PCR and ELISA are two reliable methods for detection and diagnosis of HBoV.

show abstract

“…Some studies reported no differences in clinical severity between patients hospitalized with a single infection (sole virus) and those with viral co-infection [13,47]. Others proved that more disease severity was associated with high viral load detected in a single infection [18,76].…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of Human Bocavirus in the Middle East and North Africa: Systematic Review

2021

View full text Add to dashboard Cite

The emergence of the COVID-19 pandemic highlighted the importance of studying newly emerging viruses that cause respiratory illnesses. Human bocavirus (HBoV) is one of the relatively newly discovered viruses that has been detected worldwide and causes respiratory and gastrointestinal infections, mainly in pediatric patients. However, little is known about the pathogenicity and evolution of HBoV. This systematic review was initiated to clarify the prevalence and circulating genotypes of HBoV in both respiratory and stool samples from patients of all age groups in the Middle East and North Africa (MENA) from 2005 to February 2021. We performed an electronic search through Science Direct, Scopus, PubMed, Mendeley and Cochrane Library databases. We included all studies reporting the detection rate of HBoV in the MENA region. Data were extracted, and the quality of the included articles was assessed. We included articles containing data on HBoV only or with other respiratory or gastrointestinal viral infections. Review articles, case studies, and animal and environmental studies were excluded. The final number of articles included in this study was 65 articles. The results showed that the HBoV prevalence in children was the lowest in Iran (0%) and the highest in Egypt (56.8%). In adults, the lowest and the highest prevalence were reported in Iran, with values of 0% and 6.6%, respectively. Regarding the respiratory cases, our findings revealed no significant difference between HBoV prevalence among the tested categories (p-value = 0.998). The present study has shown that HBoV is common in children and adults in the MENA region. This systematic review highlights the need for more data on the role of coinfection of HBoV and other viruses, for instance, SARS-CoV-2 in children with acute bronchiolitis.

show abstract

Detection of Human Bocavirus in Egyptian Children Suffering from Acute Lower Respiratory Tract Infection

Cited by 5 publications

References 27 publications

Detection of Human Bocavirus Infection in Children with Lower Respiratory

Detection of Human Bocavirus Infection in Children with Lower Respiratory

Detection and phylogenetic analysis of Human bocavirus in children diagnosed with acute respiratory tract infection

Epidemiology of Human Bocavirus in the Middle East and North Africa: Systematic Review

Contact Info

Product

Resources

About