Detection of gene stn in some non-typhoidal Salmonella spp. which isolated from patients with diarrhea

Abstract

The present study was conducted to estimate the prevalence of Non-typhoidal Salmonella spp. From April 2017 to September 2017 approximately 220 samples from diarrhoeic stool of human ages between one month till above sixty years were collected in Rizkary , children's and Kirkuk hospitals to determine the prevalence stn gene among Non-typhoidal Salmonella spp(S. enteritidis). A total of 220 samples were collected, The colonial morphology, staining, microscopical examination, cultural media as well as biochemical characteristics of the isolates found out the presence (72) 32.7% out of 220 salmonella species isolates, (41) 57% were belong to NTS from 72 isolates while S. enteritidis were 28(68.2%) from 41 isolates of NTS. PCR assay was carried out to detect the presence of stn gene, (24) 85.7% isolates of S. enteritidis contained the gene among 41 isolates of S. enteritidis. 100% of isolates were susceptible to Norfloxacin, while 16.7% were sensitive to Cefotaxime, 70% of isolates appeared multidrug resistance. 32.1% infections recorded in ages above sixty,25% age less than one year.
 
 http://dx.doi.org/10.25130/tjps.24.2019.005

Serotyping and Antimicrobial Resistance Profile of Enteric Nontyphoidal Salmonella Recovered from Febrile Neutropenic Patients and Poultry in Egypt

“…However, the pef A gene was detected in 18 isolates from chicken and 1 isolate from sheep. Moreover, a previous study [ 30 ] detected the presence of the stn gene in 85.7% isolates of S. Enteritidis among 41 collected isolates.…”

“…The plasmid DNA of each serovar was used as a PCR template for detection of the three major extended spectrum β-lactamases (ESBLs), including bla TEM coded for TEM extended spectrum β-lactamase, bla SHV coded for SHV extended spectrum β-lactamase, and bla CTX-m coded for cefotaxime (CTX-M) extended spectrum β-lactamase as well as the aac (6′)-Ib coded for aminoglycoside 6′-N-acetyltransferase type Ib ciprofloxacin resistant variant ( Table 9 ). Analysis of the PCR products was carried out using agarose gel electrophoresis [ 30 ].…”

“…Plasmid extraction was carried out from the ampicillin resistance serovars using iNtRON's DNA-spin™ Kit (iNtODEWORLD, Boston, MA, USA) according to its manufacturer's manual and that was followed by agarose gel electrophoresis [38] 6)-Ib coded for aminoglycoside 6 -N-acetyltransferase type Ib ciprofloxacin resistant variant (Table 9). Analysis of the PCR products was carried out using agarose gel electrophoresis [30]. The 100 serovars (50 human serovars and 50 poultry serovars) and the standard bacterial Salmonella strain were cultured onto Meuller Hinton agar and XLD agar.…”

Detection of Virulence and β-lactamase resistance genes of non-typhoidal Salmonella isolates from human and animal origin in Egypt "one health concern"

“…The invA gene is a biomarker for NTS detection and play major role in invasion of epithelial cells. Whereas, stn gene encode for an enterotoxin production and spvC encodes for systemic infection in human and replication in animal [ 10 – 12 ].…”