The present  study was conducted to estimate the prevalence of Non-typhoidal Salmonella spp. From April 2017 to September 2017 approximately 220 samples from diarrhoeic stool of human ages between one month till above sixty years were collected in Rizkary , children's and Kirkuk hospitals to determine the prevalence stn gene among Non-typhoidal Salmonella spp(S. enteritidis). A total of 220 samples were collected, The colonial morphology, staining, microscopical examination, cultural media as well as biochemical characteristics of the isolates found out the presence (72) 32.7%  out of 220 salmonella species isolates, (41) 57% were belong to NTS from 72 isolates while S. enteritidis were 28(68.2%) from 41 isolates of NTS. PCR assay was carried out to detect the presence of stn gene, (24) 85.7%  isolates of S. enteritidis contained the gene among 41 isolates of S. enteritidis. 100% of isolates were susceptible to Norfloxacin, while 16.7% were sensitive to Cefotaxime, 70% of isolates appeared multidrug resistance. 32.1% infections recorded in ages above sixty,25% age less than one year.   http://dx.doi.org/10.25130/tjps.24.2019.005

The present study was performed on a total of 350 samples  (145 freshly chiken, 20 frozen chiken, 30 skin wounds of slaughtering workers,55 nasal swaps of workers and 100 swap from healthy skins of slaughtering warkers. The specimen taken from Shops selling live chickens (local) in Kirkuk and Erbil Governorates that inspected for Staphylococcus aureus. Fifty nine S. aureus isolates were isolated after clinical assessment for bacteriological examination. The isolates were distributed as 15(25%) from heathy workers skins, 5(8.5%) from workers wounds, 20(33.9) from freshly chickens samples,4(6.8%)from frozen chiken and 15 (25%) nasal swaps. The antibiotic susceptibility showed that the most effective antibiotics was Norfloxacin followed Ciprofloxacin, Gentamycin, and trimethoprim.20 isolates were choosen for PCR test out of 59 depending on sensitivity test (20 hsolate appeared resistance to methecillin disc), PCR results showed that mecA was detected in 12(20.3%) isolate. Distributed to 5(8.5%) fresh chiken, 3(5%) wound, 2(3.4%) healthy workers skins and 1 (1.7%) to each of nasal swabs of workers and frozen chickens of studied isolates.While pvl virulence gene was detected in 6(30%) isolates out of the 12 isolates were positive to mecA gene, 3(25%) fresh chickens, 2(16.7%) healthy workers skins, 1 (8.3%) in wound workers skins, None of the MRSA isolates from nasal workers swabs and frozen chickens were found positive for the PVL genes.The aim of this study to detect PVL gene among MRSA isolates.

The present study was conducted to estimate the prevalence of Non-typhoidal Salmonella spp. From April 2017 to September 2017 approximately 222 samples from diarrhoeic stool of human ages between one month till above sixty years were collected in Rizkary , children's and Kirkuk hospitals to determine the prevalence stn gene among Non-typhoidal Salmonella spp(S. enteritidis). A total of 220 samples were collected, The colonial morphology, staining, microscopical examination, cultural media as well as biochemical characteristics of the isolates found out the presence (72) 32.7% out of 220 salmonella species isolates, (41) 57% were belong to NTS from 72 isolates while S. enteritidis were 28(68.2%) from 41 isolates of NTS. PCR assay was carried out to detect the presence of stn gene, (24) 85.7% isolates of S. enteritidis contained the gene among 41 isolates of S. enteritidis. 100% of isolates were susceptible to Norfloxacin, while 16.7% were sensitive to Cefotaxime, 70% of isolates appeared multidrug resistance. 32.1% infections recorded in ages above sixty,25% age less than one year.