Detection of gastric carcinoma-associated antigen MG7-Ag in human sera using surface plasmon resonance sensor

Fang, Xiangyi; Tie, Jun; Xie, Yonghong; Li, Quanjiang; Zhao, Qingchuan; Fan, Daiming

doi:10.1016/j.canep.2010.05.004

Cited by 21 publications

(11 citation statements)

References 12 publications

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“…The process of reactions on the sensor chip was characterized by changes in wavelength resonance. A detailed optical layout was described in our previous publications [23,24].…”

Section: Instrumental Methodsmentioning

confidence: 99%

Spectral surface plasmon resonance imaging for the detection of clenbuterol via three-dimensional immobilization of bioprobes

Yao

Fang

et al. 2015

Analytical Biochemistry

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“…The process of reactions on the sensor chip was characterized by changes in wavelength resonance. A detailed optical layout was described in our previous publications [23,24].…”

Section: Instrumental Methodsmentioning

confidence: 99%

Spectral surface plasmon resonance imaging for the detection of clenbuterol via three-dimensional immobilization of bioprobes

Yao

Fang

et al. 2015

Analytical Biochemistry

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“…The details of the spectral SPRi instrument have been reported previously [21,22]. Briefly, with the incident angle fixed, we synchronously obtained SPR spectrum curves for each Au spot on the array using scanning wavelength in the detection procedure.…”

Section: Instrumental Methodsmentioning

confidence: 99%

“…However, most SPR biosensors only provide a single sample measurement, which is time consuming and of low efficiency. Previously, we developed and reported a spectral imaging surface plasmon resonance (spectral SPRi) biosensor system [21,22] for multichannel measurement. In this work, we have applied the SPRi for CLEN detection.…”

Section: Introductionmentioning

confidence: 99%

Clenbuterol Assay by Spectral Imaging Surface Plasmon Resonance Biosensor System

Yao

Fang

et al. 2015

Appl Biochem Biotechnol

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To prevent illegal use of clenbuterol and for quality control in the food industry, more efficient and reliable methods for clenbuterol detection are needed. In this study, clenbuterol was detected using a spectral imaging surface plasmon resonance sensor system via two inhibition methods: (1) the target site compensation method, in which anti-clenbuterol antibody was immobilized on the sensor chip as a bioprobe and (2) the solution competition method in which a clenbuterol-BSA conjugate was immobilized on the sensor chip as the bioprobe. The detectable clenbuterol concentration ranged between 6.25 and 100 μg/mL for both methods. The clenbuterol limit of detection for the target site compensation method and solution competition method are estimated to be 6.7 and 4.5 μg/mL, respectively. The proposed methods were successfully applied to the detection of clenbuterol molecules and were found to have high specificity and high-throughput and were label free and operationally convenient.

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“…The sensitivity of the serum MG7-Ag was 81.4% for the diagnosis of gastric cancer (Ren et al, 2011). In the region of China with high incidence of gastric cancer, the sensitivity and specificity of serum MG7-Ag detection were 77.5% and 95.62% for the diagnosis of gastric cancer (Fang et al, 2010;). Chen et al (1992) also found that MG7-Ag was an important factor for pre-warning of gastric cancer.…”

Section: Gastric Cancer Antigen Mg7-ag (Mg7-ag)mentioning

confidence: 99%

Serum Protein and Genetic Tumor Markers of Gastric Carcinoma

He¹,

Zhang²

2013

Asian Pacific Journal of Cancer Prevention

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The high incidence of gastric cancer and consequent mortality pose severe threats to human health. Early screening, diagnosis and treatment are the key to improve the prognosis of the patients with gastric cancer. Gastroscopy with biopsy is an efficient method for the diagnosis of early gastric cancer, but the associated discomfort and high cost make it difficult to be a routine method for screening gastric cancer. Serum tumor marker assay is a simple and practical method for detection of gastric cancer, but it is limited by poor sensitivity and specificity. Therefore, people have been looking for novel serum markers of gastric cancer in recent years. Here we review the novel serum tumor markers of gastric cancer and their diagnostic significance, focusing on the discoveries from serum proteomics analyses and epigenetics researches.

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Detection of gastric carcinoma-associated antigen MG7-Ag in human sera using surface plasmon resonance sensor

Cited by 21 publications

References 12 publications

Spectral surface plasmon resonance imaging for the detection of clenbuterol via three-dimensional immobilization of bioprobes

Spectral surface plasmon resonance imaging for the detection of clenbuterol via three-dimensional immobilization of bioprobes

Clenbuterol Assay by Spectral Imaging Surface Plasmon Resonance Biosensor System

Serum Protein and Genetic Tumor Markers of Gastric Carcinoma

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