Detection and typing of human papillomavirus in cutaneous warts of patients infected with human immunodeficiency virus type 1

Porro, Adriana María; Alchorne, M. M. A.; Mota, Giana; Michalany, Nílceo Schwery; Pignatari, Antônio Carlos Campos; Souza, IE

doi:10.1111/j.1365-2133.2003.05650.x

Cited by 43 publications

(51 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Only Harwood and coworkers (13) have described the presence of beta-PV types in cutaneous warts of immunosuppressed patients. The nu-PV genus consists of only one HPV type (HPV41), which was originally isolated from a facial wart (11).In general, cutaneous warts from immunocompetent and immunocompromised patients appear to show the same genotype distributions (16,19). This has to be confirmed by a large-scale study comparing the distributions in the two populations with the same techniques.…”

mentioning

confidence: 65%

See 1 more Smart Citation

Evaluation of a Novel Broad-Spectrum PCR-Multiplex Genotyping Assay for Identification of Cutaneous Wart-Associated Human Papillomavirus Types

Koning

Schegget

Eekhof³

et al. 2010

J Clin Microbiol

View full text Add to dashboard Cite

A large number of human papillomavirus (HPV) types, distributed over five papillomavirus genera, are detectable in the skin. HPV types belonging to the alpha, gamma, and mu genera have been detected in cutaneous warts. A state-of-the-art HPV genotyping assay for these cutaneous wart-associated HPV types does not exist although warts constitute a highly prevalent skin condition, especially in children (33%) and organ transplant recipients (45%). Cutaneous warts are again the focus of attention as their clinical relevance rises with the increasing number of chronically immunosuppressed patients. The objective of this study was to develop and evaluate a DNA-based genotyping system for all known cutaneous wart-related HPV types using PCR and Luminex xMAP technology. The broad-spectrum PCR amplified DNA of all known wart-associated HPV types from the genera alpha (HPVs 2, 3, 7, 10, 27, 28, 29, 40, 43, 57, 77, 91, and 94), gamma (HPVs 4, 65, 95, 48, 50, 60, and 88), mu (HPVs 1 and 63), and nu (HPV41). The probes were evaluated using plasmid HPV DNA and a panel of 45 previously characterized cutaneous wart biopsy specimens showing high specificity. HPV was also identified in 96% of 100 swabs from nongenital cutaneous warts. HPV types 1, 2, 27, and 57 were the most prevalent HPV types detected in 89% of the swabs. In conclusion, this Luminex-based genotyping system identifies all known cutaneous wart HPV types including phylogenetically related types, is highly HPV type specific, and is suitable for large-scale epidemiological studies.Papillomaviruses (PV) constitute a group of viruses associated with benign and malignant proliferative lesions of cutaneous and mucosal epithelia. The taxonomic family of PV includes 16 genera (5). More than 100 human papillomavirus (HPV) types have been fully sequenced. The number of HPV types that are not yet fully characterized is probably much larger. HPV types, distributed over five genera (alpha, beta, gamma, mu, and nu) and 16 species, infect the skin (5). HPV types belonging to species of three genera (alpha, gamma, and mu) have most frequently been detected in hyperkeratotic skin lesions (warts).The alphapapillomavirus (alpha-PV) types infecting the genital mucosa (e.g., HPV16, HPV18, HPV6, and HPV11) are the best understood. HPV16 and HPV18 are the most prevalent types involved in the pathogenesis of anogenital (e.g., cervical) cancer, and HPV types 6 and 11 cause genital warts and laryngeal papillomas. The alpha-PV types belonging to species 2, 4, and 8, which have been detected in cutaneous warts, have been studied less thoroughly. At present the most frequently detected alpha-PV types in cutaneous warts are HPV types 2, 3, 10, 27, and 57 (3, 10, 12-16, 18, 19). Gamma-PV types, including HPV4, HPV60, and HPV65, have also regularly been detected in cutaneous warts (4,10,12,15). Mu-PV types, HPV1 and HPV63, have also been detected in cutaneous warts (7). HPV types included in the beta and nu genera have rarely been detected in cutaneous warts. Only Harwood and coworkers (13) hav...

show abstract

mentioning

confidence: 65%

“…In general, cutaneous warts from immunocompetent and immunocompromised patients appear to show the same genotype distributions (16,19). This has to be confirmed by a large-scale study comparing the distributions in the two populations with the same techniques.…”

mentioning

confidence: 79%

Evaluation of a Novel Broad-Spectrum PCR-Multiplex Genotyping Assay for Identification of Cutaneous Wart-Associated Human Papillomavirus Types

Koning

Schegget

Eekhof³

et al. 2010

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…They also reported the results of a detection study of HPV genotype infection rates using 75 biopsy samples including German, Japanese, and Singaporean patients, that is, 32 cases of HPV-57, 27 cases of HPV-2, and 16 cases of HPV-27 [Chen et al, 1993]. Porro et al [2003] reported that HPV DNA was detected in 64% of cutaneous warts from HIVinfected patients (n ¼ 24) and in 79% of samples from control non-HIV-infected patients (n ¼ 13), and added that HPV-2/27/57 predominated in both groups being present in 81% of the lesions from the HIV-infected patients and 82% of the samples from the non-HIVinfected patients. It is said that there are environmental factors associated with acquisition of warts.…”

Section: Discussionmentioning

confidence: 96%

A genotype distribution of human papillomaviruses detected by polymerase chain reaction and direct sequencing analysis in a large sample of common warts in Japan

Hagiwara

Uezato

Arakaki

et al. 2005

Journal of Medical Virology

View full text Add to dashboard Cite

There have been no large-scale epidemiological studies of human papillomavirus (HPV) genotype distribution of common warts in Japan. A total of 213 patients with common warts (104 males and 109 females) in Japan were studied to detect HPV genotype distribution by polymerase chain reaction (PCR) and direct sequencing analysis. The results were as follows: 94 HPV-1a (44.1%), 35 HPV-4 (16.4%), 30 HPV-65 (14.1%), 13 HPV-27 (6.1%), 13 HPV-2a (6.1%), 9 HPV-57b (4.22%), 3 HPV-16 (1.41%), 2 HPV-6a (0.94%), 2 HPV-63 (0.94%), and 1 case for each of HPV-3, -5, -5b, -7, -10, -21, -29, -47, -56, -57, -62, and -92 (0.47%, respectively). Four cases (1.88%) were found in which two different HPV types were detected within the lesions: one case of HPV-1a with HPV-16, one case of HPV-1a with HPV-65, one case of HPV-6a with HPV-8, and one case of HPV-65 with HPV-16. There were seven cases of mucosal types (3.3%), that is, two HPV-6a, three HPV-16, one HPV-56, and one HPV-62, and three cases of epidermodysplasia verruciformis (EV)-related types (1.41%), that is, one HPV-5, one HPV-5b (both of which belonged to a high-risk group), and one HPV-47 (which belonged to a low-risk group). To date, this is the largest sequencing-based study of HPV for common warts in Japan. It is said that common warts are induced predominantly by HPV-2, -27, and -57 in European population. However, the present results showed that in Japan they were induced mostly by HPV-1, -4, and -65. This suggests that regional differences in HPV genotype distribution may exist between European and Japanese populations.

show abstract

“…Skin warts are considered the main manifestation of the cutaneous HPV types, with HPV 1, 2, 3, 4, 27, and 57 being detected most frequently [10,43] (Table 1). Although cutaneous HPV types are predominantly detected in skin warts, the presence of mucosal HPVs has also been reported; however, the origin and the role of these types on the skin remain unclear [10,31,43].…”

Section: Skin Wartsmentioning

confidence: 99%

Human papilloma virus (HPV) infection in children and adolescents

2008

View full text Add to dashboard Cite

Human papilloma viruses (HPV) are common pathogens associated with a wide range of cutaneous and mucosal infections in childhood. Different HPV types can cause common warts, genital warts, low-grade as well as high-grade squamous intraepithelial lesions. Anogenital warts represent an issue with legal and clinical implications and evaluation of children for the possibility of sexual abuse should be considered in all cases. Recurrent respiratory papillomatosis has also been associated with HPV infection in a variety of studies. The recently introduced HPV vaccination is expected to prevent HPV-related cervical cancer in adulthood; however, HPV infection will continue to affect children.

show abstract

Detection and typing of human papillomavirus in cutaneous warts of patients infected with human immunodeficiency virus type 1

Abstract: This is the first study evaluating the prevalence of HPV types in cutaneous warts of HIV-infected patients and immunocompetent individuals in Brazil.

Cited by 43 publications

References 51 publications

Evaluation of a Novel Broad-Spectrum PCR-Multiplex Genotyping Assay for Identification of Cutaneous Wart-Associated Human Papillomavirus Types

Evaluation of a Novel Broad-Spectrum PCR-Multiplex Genotyping Assay for Identification of Cutaneous Wart-Associated Human Papillomavirus Types

A genotype distribution of human papillomaviruses detected by polymerase chain reaction and direct sequencing analysis in a large sample of common warts in Japan

Human papilloma virus (HPV) infection in children and adolescents

Contact Info

Product

Resources

About