A genotype distribution of human papillomaviruses detected by polymerase chain reaction and direct sequencing analysis in a large sample of common warts in Japan

Hagiwara, Keisuke; Uezato, Hiroshi; Arakaki, Hajime; Nonaka, Shigeo; Nonaka, Kimiko; Nonaka, Hideo; Asato, Tsuyoshi; Oshiro, Minoru; Kariya, Ken-ichi; Hattori, Akira

doi:10.1002/jmv.20421

Cited by 44 publications

(49 citation statements)

References 20 publications

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“…HPV-27, -57 and -1 were also prevalent, showing similar results with the German [11] and Chinese study [13]. However, Hagiwara et al [9] reported that the most frequent HPV types found in common warts among Japanese patient were HPV-1, 4, and 65. For the 20 samples with unidentified HPV, primers for HPV-7, 10 and 65 were added for trial.…”

Section: Specificity and Sensitivitysupporting

confidence: 81%

“…Especially, HPV-2, -27, and -57 are closely related and associated with skin warts. Among them, we chose common warts-related 6 HPV subtypes for identification by the multiplex PCR [3,9]. To set up a multiplex PCR for rapid identification of HPV-1, -2, -3, -4, 27, and -57, type-specific primers were designed from the intergenic region of each viral genome (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Detection and Typing of Human Papillomavirus in Cutaneous Common Warts by Multiplex Polymerase Chain Reaction

Choi¹,

Lim²,

Kim³

et al. 2011

Journal of Life Science

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A number of epidemiological studies have identified human papillomavirus (HPV) types 1, 2, 3, 4, 7, 10, 27, 57, and 65 in cutaneous common warts. However, identification of the HPV subtype by conventional polymerase chain reaction (PCR) is time consuming with its multi-step laboratory process. In this study, we aim to develop a specific one-step multiplex polymerase chain reaction method which capably identifies six different HPV genotypes related to common warts. By HPV DNA sequence analysis, 6 pairs of specific primers were designed from the intergenic regions of genes L1 to E6, and from genes E2 to L2. DNA sequence analysis with the L1 gene sequence of the sample was performed to measure the specificity of multiplex PCR. HPV-1, -2, -3, -4, -27, and -57 were identified without cross amplification in 109 out of 129 samples. The sensitivity and specificity of our set of primers in detecting HPV were 85% and 99.5%, respectively. For the 20 samples where HPV type was not identifiable by our batch of primer sets, multiplex PCR with an additional set of HPV primers was done, where 7 were found positive for HPV-7 or -65. Our results demonstrate that the newly designed multiplex PCR can rapidly detect the specific HPV subtype involved in common warts with high accuracy.

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Section: Specificity and Sensitivitysupporting

confidence: 81%

Section: Resultsmentioning

confidence: 99%

Detection and Typing of Human Papillomavirus in Cutaneous Common Warts by Multiplex Polymerase Chain Reaction

Choi¹,

Lim²,

Kim³

et al. 2011

Journal of Life Science

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“…At present the most frequently detected alpha-PV types in cutaneous warts are HPV types 2, 3, 10, 27, and 57 (3, 10, 12-16, 18, 19). Gamma-PV types, including HPV4, HPV60, and HPV65, have also regularly been detected in cutaneous warts (4,10,12,15). Mu-PV types, HPV1 and HPV63, have also been detected in cutaneous warts (7).…”

mentioning

confidence: 99%

“…These studies utilized different detection methods, such as general primermediated PCR followed by HPV typing with direct sequencing (12,15) or by restriction enzyme cleavage of the PCR product (18). Other methods employed were multiple-type-specific PCR combined with direct sequencing (3), in situ hybridization (8), and (Southern) blot hybridization (6,10).…”

mentioning

confidence: 99%

Evaluation of a Novel Broad-Spectrum PCR-Multiplex Genotyping Assay for Identification of Cutaneous Wart-Associated Human Papillomavirus Types

Koning

Schegget

Eekhof³

et al. 2010

J Clin Microbiol

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A large number of human papillomavirus (HPV) types, distributed over five papillomavirus genera, are detectable in the skin. HPV types belonging to the alpha, gamma, and mu genera have been detected in cutaneous warts. A state-of-the-art HPV genotyping assay for these cutaneous wart-associated HPV types does not exist although warts constitute a highly prevalent skin condition, especially in children (33%) and organ transplant recipients (45%). Cutaneous warts are again the focus of attention as their clinical relevance rises with the increasing number of chronically immunosuppressed patients. The objective of this study was to develop and evaluate a DNA-based genotyping system for all known cutaneous wart-related HPV types using PCR and Luminex xMAP technology. The broad-spectrum PCR amplified DNA of all known wart-associated HPV types from the genera alpha (HPVs 2, 3, 7, 10, 27, 28, 29, 40, 43, 57, 77, 91, and 94), gamma (HPVs 4, 65, 95, 48, 50, 60, and 88), mu (HPVs 1 and 63), and nu (HPV41). The probes were evaluated using plasmid HPV DNA and a panel of 45 previously characterized cutaneous wart biopsy specimens showing high specificity. HPV was also identified in 96% of 100 swabs from nongenital cutaneous warts. HPV types 1, 2, 27, and 57 were the most prevalent HPV types detected in 89% of the swabs. In conclusion, this Luminex-based genotyping system identifies all known cutaneous wart HPV types including phylogenetically related types, is highly HPV type specific, and is suitable for large-scale epidemiological studies.Papillomaviruses (PV) constitute a group of viruses associated with benign and malignant proliferative lesions of cutaneous and mucosal epithelia. The taxonomic family of PV includes 16 genera (5). More than 100 human papillomavirus (HPV) types have been fully sequenced. The number of HPV types that are not yet fully characterized is probably much larger. HPV types, distributed over five genera (alpha, beta, gamma, mu, and nu) and 16 species, infect the skin (5). HPV types belonging to species of three genera (alpha, gamma, and mu) have most frequently been detected in hyperkeratotic skin lesions (warts).The alphapapillomavirus (alpha-PV) types infecting the genital mucosa (e.g., HPV16, HPV18, HPV6, and HPV11) are the best understood. HPV16 and HPV18 are the most prevalent types involved in the pathogenesis of anogenital (e.g., cervical) cancer, and HPV types 6 and 11 cause genital warts and laryngeal papillomas. The alpha-PV types belonging to species 2, 4, and 8, which have been detected in cutaneous warts, have been studied less thoroughly. At present the most frequently detected alpha-PV types in cutaneous warts are HPV types 2, 3, 10, 27, and 57 (3, 10, 12-16, 18, 19). Gamma-PV types, including HPV4, HPV60, and HPV65, have also regularly been detected in cutaneous warts (4,10,12,15). Mu-PV types, HPV1 and HPV63, have also been detected in cutaneous warts (7). HPV types included in the beta and nu genera have rarely been detected in cutaneous warts. Only Harwood and coworkers (13) hav...

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“…Studies in the European population have shown that common warts are induced predominantly by HPV-2, HPV-27, and HPV-57 and that EV patients are commonly linked to the oncogenic HPV-5 and HPV-8 types. However, regional differences in HPV distribution and pathogenesis have also been demonstrated [13]. We did not find any studies correlating HPV-genotype and EV in Brazilian populations.…”

Section: Discussionmentioning

confidence: 55%

Clinical presentation of tuberculoid leprosy in an epidermodysplasia verruciformis patient

Prestes-Carneiro

Nai

Silva

et al. 2011

J Infect Dev Ctries

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Epidermodysplasia verruciformis (EV) is triggered by a variety of mechanisms that at least partly include genetic background. We present a Brazilian man with a 30-year history of flat, wart-like lesions with clinical, histopathological, and evolutive aspects consistent with papillomavirus (HPV)-associated EV. Histological analysis of the wart lesions showed epidermis with hyperkeratosis, regular acanthosis, hypergranulosis, and cells with abundant basophilic cytoplasm. Moreover, a perivascular lymphocytic infiltrate was found in the superficial dermis, consistent with a viral wart. Type-2-HPV DNA was detected in various fragments of skin-wart lesions using the polymerase chain reaction (PCR). Two years after the EV diagnosis, the patient presented with an anesthetic well-demarcated, erythematous and mildly scaly plaque on his right forearm. A histopathological analysis of this lesion demonstrated the presence of a compact tuberculoid granuloma. ZiehlNeelsen staining demonstrated the presence of rare acid-fast bacilli and confirmed the tuberculoid leprosy diagnosis. The patient's Mitsuda Intradermal Reaction was positive. To elucidate the possible mechanism involved in this case of EV, we genotyped the HLA genes of this patient. DQB genotyping showed the polymorphic HLA alleles DQB1*0301 and 0501. The patient was treated with a paucibacillary multidrug therapy scheme, and the disease was cured in six months. This report describes an EV patient with an M. leprae infection, confirming that tuberculoid leprosy patients possess a relatively specific and efficient cell-mediated immunity against the bacillus and, therefore, localized forms of the disease. Moreover, we show the possible involvement of the polymorphic HLA alleles DQB1*0301 and 0501 in EV induction mechanisms.

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A genotype distribution of human papillomaviruses detected by polymerase chain reaction and direct sequencing analysis in a large sample of common warts in Japan

Cited by 44 publications

References 20 publications

Detection and Typing of Human Papillomavirus in Cutaneous Common Warts by Multiplex Polymerase Chain Reaction

Detection and Typing of Human Papillomavirus in Cutaneous Common Warts by Multiplex Polymerase Chain Reaction

Evaluation of a Novel Broad-Spectrum PCR-Multiplex Genotyping Assay for Identification of Cutaneous Wart-Associated Human Papillomavirus Types

Clinical presentation of tuberculoid leprosy in an epidermodysplasia verruciformis patient

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