Detection and molecular characterization of the cucumber mosaic virus in chili pepper (Capsicum spp. L.) crops

Abstract

The chili pepper (Capsicum spp. L.) is a vegetable of economic importance that has been affected worldwide by the cucumber mosaic virus (CMV), a pathogen that causes a devastating disease in this crop. The aim of this research was the detection and characterization of CMV in chili pepper crops in Valle del Cauca, Colombia. Leaves of three chili pepper varieties (tabasco, cayenne and habanero) with viral symptoms were collected in four municipalities of Valle del Cauca. Total RNA was purified and a fragment of capsid protein (CP) from CMV was amplified by RT‑PCR. Then, it was sequenced and bioinformatically analyzed, and from these sequences, specific primers were designed. From 71 chili pepper samples collected in Palmira, Yumbo, Vijes and Yotoco, 37 were positive for CMV (52.1%). The CMV chili pepper sequence analysis showed that they had their highest identity (98.5%) with a CMV isolated from bananas in Ecuador. Specific primers designed for CMV chili pepper showed greater sensitivity for detecting this virus (64.7% vs. 52.1%). The CMV chili pepper CP analysis indicated that it could be transmitted by the species Aphis gossypii. This r the first time, the molecular characterization of CMV in three chili pepper varieties.

Genetic Diversity of CMV and ToMV Isolates in Tomato and Pepper Production Areas in Hakkari, Turkey

“…CMV sequence analysis showed the maximum identity (98.5%) of the isolates in the study was with a CMV isolated from bananas from Ecuador. CP analysis of CMV also demonstrated that it can be transmitted by the species Aphis gossypii [43]. CMV is one of the most harmful viruses causing disease outbreaks in agricultural products all around the world [21].…”

Detección molecular de Cucumber mosaic virus y Pepper severe mottle virus en arvenses asociadas al cultivo de ají (Capsicum spp.)

“…121 arvenses fueron colectadas en nueve lotes de cultivo de ají (Capsicum spp. ), localizados en siete municipios del Departamento del Valle del Cauca, Colombia: Bolívar (19 plantas), Candelaria (17), La Unión (7), Roldanillo (25), Tuluá (38), Vijes (10) y Yumbo (5) (Figura 1). La mayoría de las muestras (113) fueron asintomáticas y solo 21 presentaron síntomas de infección viral: deformación en hojas, epinastia, clorosis o mosaicos dorados (Tabla 1; Figura 2c, 2j, 2i).…”

İzmir ilinde domates ve biber bitkilerini etkileyen tomato spotted wilt virus (TSWV) ve cucumber mosaic virus (CMV) etmenlerinin moleküler karakterizasyonu

“…In Rwanda, hot pepper genotypes were evaluated for resistance to CMV under screenhouse conditions by ELISA and RT-PCR, and all commercial genotypes were found to be susceptible (Waweru et al, 2020). CMV infection rate was reported as 64.7% in evaluated chili pepper varieties in Colombia by using molecular techniques (Rivera-Toro et al, 2020). More recently, chili pepper is reported to be affected by CMV with 47.91% infection rate in India (Vinodhini et al, 2021).…”