Detection and Clonal Analysis of Anaerobic Bacteria Associated to Endodontic‐Periodontal Lesions

Abstract

Different clones of Pi and Pg with a high intraspecific genotype similarity were found to colonize the same anatomic sites in endodontic-periodontal infections.

Bacteria Community Study of Combined Periodontal‐Endodontic Lesions Using Denaturing Gradient Gel Electrophoresis and Sequencing Analysis

“…Compared with conventional culture methods, molecular techniques have the advantage of detecting uncultivable bacteria or ones that are difficult to grow. However, only expected species have been investigated with any frequency because the number of target bacteria was restricted for investigations using polymerase chain reaction (PCR) 9‐11 . For example, Rupf et al 10 used molecular techniques to detect several expected species in combined periodontal‐endodontic lesions of the same tooth; they concluded that periodontal pathogens often accompany endodontic infections.…”

“…Given these previous studies, 5‐11 a hypothesis arose that the adjacent PP is a likely source for non‐carious RC infection associated with combined periodontal‐endodontic lesions. The aim of the present study is to identify the entire microbial population obtained from RCs and from subgingival plaque samples of teeth associated with combined periodontal‐endodontic lesions, using PCR‐based 16S ribosomal RNA (rRNA) gene DGGE and sequencing.…”

Porphyromonas endodontalisin chronic periodontitis: a clinical and microbiological cross-sectional study

“…This species, along with other black-pigmented anaerobic rods, has been implicated in the etiology of infected root canals and periodontitis (4). However, additional studies are required to determine the role of P. endodontalis in patients with chronic periodontitis but without periapical lesions, as well as the contribution of this microorganism to the pathogenesis of periodontitis (10). …”

In vitro evaluation of the antimicrobial activity of nanosilver-mineral trioxide aggregate against frequent anaerobic oral pathogens by a membrane-enclosed immersion test

“…[1][2][3] The predominant bacterial genera responsible for endodontic-periodontal infections include Fusobacterium, Porphyromonas, Aggregatibacter, and Prevotella. [4] Mineral trioxide aggregate (MTA) is currently the material of choice for repairing root canal perforations and endodontic treatment. [5,6] MTA is a water-based biocompatible material that promotes regeneration of surrounding tissues.…”