Bacteria Community Study of Combined Periodontal‐Endodontic Lesions Using Denaturing Gradient Gel Electrophoresis and Sequencing Analysis

Li, Hong; Guan, Rui; Sun, Jinghua; Hou, Benxiang

doi:10.1902/jop.2014.130572

Cited by 25 publications

(32 citation statements)

References 34 publications

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“…The microbiota of the root canal in endodonticperiodontal lesions was similar to that found in the periodontal pocket, suggesting a pathway of infection between the pulp and periodontal tissues, particularly through the apical foramen, accessory canals and/or dentinal tubules exposed to the buccal environment, corroborating previous studies (2,(4)(5)(6)(7)(8). Thus, the infected periodontal pocket was probably the main source of root canal infection in these cases of endoperiodontal lesions.…”

Section: Discussionsupporting

confidence: 88%

“…Such infections are typically multispecies and interactions among different microorganisms may contribute to the development of endodontic and periodontal flora (3). Studies that investigated teeth with periodontal and endodontic infection demonstrated that the microorganisms present in the root canals with advanced periodontal disease were similar to those found in the adjacent periodontal pockets (2,(4)(5)(6)(7)(8). Furthermore, Didilescu et al (9) investigated the association of six bacterial species in endodontic-periodontal lesions and found that the species Fusobacterium nucleatum, Capnocytophaga sputigena and Parviromona micra were more prevalent, suggesting that these species may play a role in the pathogenesis of these lesions.…”

Section: Introductionmentioning

confidence: 99%

“…Until now, there are few studies regarding bacterial community of endodontic-periodontal lesions in the literature (2,(4)(5)(6)(7)9) and, consequently, treatment protocols of these lesions are not well established in clinical practice. The qualitative and quantitative correlation of pathogens and endotoxins in the infection processes of endodonticperiodontal lesions may offer a better understanding of the disease and it is essential for the development of improved strategies for diagnosis and therapeutic intervention.…”

Section: Introductionmentioning

confidence: 99%

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Microbial Profile and Endotoxin Levels in Primary Periodontal Lesions with Secondary Endodontic Involvement

et al. 2019

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This study was carried out to investigate the microbial profile and endotoxin levels of endodontic-periodontal lesions of periodontal origin. Periodontal and endodontic samples were taken from periodontal pockets and necrotic root canals of 10 teeth with endodontic-periodontal lesions. Evidencing of 40 different bacterial species were determined in each endodontic and periodontal sample using the checkerboard DNA-DNA hybridization method and Kinetic chromogenic LAL assay was used for quantification of endotoxins. Fisher’s exact test correlated the bacterial species with the endodontic or periodontal microbiota. The endotoxin levels (EU/mL) found in samples of the root canal and periodontal pocket were compared by the Wilcoxon test (p<0.05). Bacteria and LPS units were found in 100% of the endodontic and periodontal samples. The species E. faecium, P. acnes, G. morbillorum, C. sputigena and L. buccalis were strongly correlated with the endodontic microbiota and P. nigrescens with the periodontal microbiota. P. intermedia, P. endodontalis and V. parvula were more prevalent in both endodontic and periodontal microbiots. The endotoxin levels in the periodontal pocket (89600 EU/mL) were significantly higher than in the root canal (2310 EU/mL). It was concluded that the microbiota present in the periodontal and endodontic tissues is similar, with a higher prevalence of species of the orange complex and a higher level of endotoxin in the periodontal pockets.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microbial Profile and Endotoxin Levels in Primary Periodontal Lesions with Secondary Endodontic Involvement

et al. 2019

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“…The latter type occurs less frequently and is usually referred to as a "true-combined" or "combined" lesion. 89,90 These lesions may develop in subjects with periodontal health [91][92][93] or disease 94,95 ( Table 6). The periodontal condition has an important impact in the prognosis of the EPL because of the striking changes in the oral ecology of subjects with periodontal diseases.…”

Section: Primary Etiologymentioning

confidence: 99%

Acute periodontal lesions (periodontal abscesses and necrotizing periodontal diseases) and endo‐periodontal lesions

Herrera

Retamal‐Valdes

Alonso

et al. 2018

J Clinic Periodontology

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Objective To critically evaluate the existing literature on acute lesions occurring in the periodontium (periodontal abscesses [PA], necrotizing periodontal diseases [NPD], and endo‐periodontal lesions [EPL]) to determine the weight of evidence for the existence of specific clinical conditions that may be grouped together according to common features. The ultimate goal is to support an objective classification system. Importance Although PA, NPD, and EPL occur with relatively low frequency, these lesions are of clinical relevance, because they require immediate management and might severely compromise the prognosis of the tooth. Findings In general, the evidence available to define these three conditions was considered limited. PA and EPL are normally associated with deep periodontal pockets, bleeding on probing, suppuration, and almost invariably, with pain. EPL are also associated with endodontic pathology. NPDs have three typical features: pain, bleeding, and ulceration of the gingival interdental papilla. The available data suggested that the prognosis of PA and EPL are worse in periodontitis than in nonperiodontitis patients. Lesions associated with root damage, such as fractures and perforations, had the worst prognosis. NPD progression, extent and severity mainly depended on host‐related factors predisposing to these diseases. Conclusions PA should be classified according to the etiological factors involved, with the most frequent being those occurring in pre‐existing periodontal pockets. NPD are clearly associated with the host immune response, which should be considered in the classification system for these lesions. EPLs should be classified according to signs and symptoms that have direct impact on their prognosis and treatment, such as presence or absence of fractures and perforations, and presence or absence of periodontitis.

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“…Filifactor belongs to Firmicutes and contains a few pathogenic species (e.g. F. alocis ) that are associated with periodontal diseases and endodontic lesions [21, 22]. This results suggested that Filifactor either represented part of the oral microbiota from the sampling, or could thrive as pathogens in other body sites like many other oral microbes did (e.g.…”

Section: Resultsmentioning

confidence: 99%

Alterations of human lung and gut microbiome in non-small cell lung carcinomas and distant metastasis

Lü

Gao

Wei

et al. 2020

Preprint

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25Background 26Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related deaths 27 worldwide. Although dysbiosis of lung and gut microbiota have been associated with 28 NSCLC, their relative contributions are unclear; in addition, their roles in distant metastasis 29 (DM) are still illusive. 30 Results 31We surveyed the fecal and sputum (as a proxy for lung) microbiota in healthy controls and 32 NSCLC patients of various stages, and found significant perturbations of gut-and sputum-33 microbiota in patients with NSCLC and DM. Machine-learning models combining both 34 microbiota (mixed models) performed better than either dataset in patient stratification, 35with the highest area under the curve (AUC) value of 0.842. Sputum-microbiota 36 contributed more than the gut in the mixed models; in addition, sputum-only models 37 performed similarly to the mixed models in most cases. Several microbial-biomarkers were 38 shared by both microbiota, indicating their similar roles at distinct body sites. 39Microbial-biomarkers of distinct disease stages were mostly shared, suggesting 40 biomarkers for distant metastasis could be acquired early. Furthermore, Pseudomonas 41 aeruginosa, a species previously associated with wound infections, was significantly more 42 3 abundant in brain metastasis, indicating distinct types of DMs could have different 43 microbial-biomarkers. 44 Conclusion 45Our results indicate that alterations of sputum-microbiota have stronger relationships with 46 NSCLC and distant metastasis than the gut, and strongly support the feasibility of 47 metagenome-based non-invasive disease diagnosis and risk evaluation. 48 49 Keywords: gut microbiota, lung microbiota, machine learning, patient stratification, 50 NSCLC, distant metastasis, brain metastasis 51 52 4 53 Background 54 Lung cancer (LC) is the leading cause of cancer-related deaths mortality worldwide, with 55 non-small cell lung cancer (NSCLC) being the most common form of LC [1]. Despite the 56 recent development of therapies for NSCLC, tumor metastasis is the main cause of 57 recurrence and mortality in patients with NSCLC [1]. One of the key challenges is the low 58 heritability of lung cancer susceptibility revealed by genetic studies: although numerous 59 studies have established the important roles of somatic mutations as well as inheritable 60 familial risks [2, 3], the genetic influence can only explain 3~15% of the heritability [4, 5], 61 depending on the surveyed population. 62 Conversely, non-genetic factors, including life styles, environmental factors and lung 63 and gut microbes are believed to contribute mostly to the disease. Especially, numerous 64 recent studies have shown that both lung and gut microbiota are involved in the 65 development of LC [6-8]. For example, researchers have used samples from 66 bronchoalveolar fluid (BALF), tissues and spontaneous sputum of lung cancer patients for 67 bacterial identification and microbiome characterization [7, 9-11]. When compared with 68 healthy controls, researchers have id...

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Bacteria Community Study of Combined Periodontal‐Endodontic Lesions Using Denaturing Gradient Gel Electrophoresis and Sequencing Analysis

Abstract: The high similarity in the sets of organisms present in both RC and PP samples in this study suggests that the pocket could be a source of RC infection. The data also demonstrate that combined periodontal-endodontic lesions consist of a diverse and complex microbial community.

Cited by 25 publications

References 34 publications

Microbial Profile and Endotoxin Levels in Primary Periodontal Lesions with Secondary Endodontic Involvement

Microbial Profile and Endotoxin Levels in Primary Periodontal Lesions with Secondary Endodontic Involvement

Acute periodontal lesions (periodontal abscesses and necrotizing periodontal diseases) and endo‐periodontal lesions

Alterations of human lung and gut microbiome in non-small cell lung carcinomas and distant metastasis

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