Detecção dos genes codificantes da toxina CDT, e pesquisa de fatores que influenciam na produção de hemolisinas em amostras de Campylobacter jejuni de origem avícola

Trindade, Michele Martins; Perdoncini, Gustavo; Sierra-Arguello, Yuli M.; Lovato, Maristela; Borsoi, Anderlise; Nascimento, Vladimir Pinheiro do

doi:10.1590/s0100-736x2015000800002

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…The cytolethal distending toxin (CDT) is encoded by three adjacent genes, cdtA, cdtB and cdtC, and is involved in cell cycle arrest in the G2/M phase [62]. This cytotoxin induces a cytoplasmic distension, leading to cell death by apoptosis about three to four days after infection, contributing to the development and pathogenesis of inflammatory diarrhea caused by campylobacteriosis in humans [63].…”

Section: Virulence Of Campylobacter Isolatesmentioning

confidence: 99%

Agents of Campylobacteriosis in Different Meat Matrices in Brazil

Takeuchi

Melo

Dumont

et al. 2022

IJERPH

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We aimed to identify the prevalence of thermophilic species of Campylobacter in meats of different species available on the Brazilian commercial market and to determine the genetic diversity, antimicrobial resistance and virulence potential of the isolates. A total of 906 samples, including chicken, beef and pork carcasses and chicken and beef livers, were purchased in retail outlets, and prevalences of 18.7% (46/246), 3.62% (5/138), 10.14% (14/138), 3.62% (5/138) and 4.47% (11/132), respectively, were identified, evidencing the dissemination of genotypes in the main producing macro-regions. Of all isolates, 62.8% were classified as multidrug resistant (MDR), with resistance to amoxicillin-clavulanate (49.4%), tetracycline (51.8%) and ciprofloxacin (50.6%) and co-resistance to macrolides and fluoroquinolones (37.1%). Multivirulent profiles were identified mainly in isolates from chicken carcasses (84.8%), and the emergence of MDR/virulent strains was determined in pork isolates. All isolates except those from chicken carcasses showed a high potential for biofilm formation (71.4% luxS) and consequent persistence in industrial food processing. For chicken carcasses, the general virulence was higher in C. jejuni (54.3%), followed by C. coli (24%) and Campylobacter spp. (21.7%), and in the other meat matrices, Campylobacter spp. showed a higher prevalence of virulence (57.2%). The high rates of resistance and virulence reinforce the existence of strain selection pressure in the country, in addition to the potential risk of strains isolated not only from chicken carcasses, but also from other meat matrices.

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Section: Virulence Of Campylobacter Isolatesmentioning

confidence: 99%

Agents of Campylobacteriosis in Different Meat Matrices in Brazil

Takeuchi

Melo

Dumont

et al. 2022

IJERPH

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“…These bacteria colonize the gut of a wide range of hosts, such as chickens, turkeys, ducks and pigs as a commensal. Notwithstanding, C. jejuni emerged as the major bacterial cause of foodborne diseases in several industrialized countries [22][23][24].…”

Section: Review Articlementioning

confidence: 99%

Microbial Resistance Due to the Use of Antimicrobials in Livestock and Agriculture

Cavalcanti

2018

APDV

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Antimicrobials are widely used in agriculture and livestock, nevertheless their large-scale and indiscriminate use contributes considerably to the development of microbial resistance. The intense commercialization of agricultural products accelerates the dissemination of pathogens that present resistance profiles, which characterize them as extremely harmful to plants and animals. Thus, there is an urgent need to control the use of antimicrobials in animal and vegetable food products in order to find new agents that are less able to develop resistance. It is essential the guidance of the professionals and the population about the rational use of antimicrobials in agriculture and livestock for the maintenance and protection of the human healthy and environment.

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Presence of genes associated with adhesion, invasion, and toxin production inCampylobacter jejuniisolates and effect of temperature on their expression

et al. 2019

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The aims of this study were to evaluate the presence of genes associated with adhesion (cadF), invasion (ciaB), and cytotoxin production (cdtA, cdtB, and cdtC) among Campylobacter jejuni isolates from a poultry slaughterhouse and to investigate the effect of different temperatures on the expression of these virulence-associated genes. A total of 88 C. jejuni isolates from cecum, liver, chicken carcasses, chilled water, and scalding water were submitted to PCR assay for detection of virulence genes. Representative isolates were selected for gene expression evaluation at 37 and 42 °C, according to their virulence gene profile and genotypic typing. All C. jejuni isolates carried the five virulence-associated genes, which play an important role in the infectious process. Differential gene expression by RT–qPCR was observed among C. jejuni isolates at 37 and 42 °C. The expression levels at 37 °C showed upregulation of the ciaB, cdtA, cdtB, and cdtC genes in five isolates, with the exception of ciaB for isolate 4. At 42 °C, upregulation was observed for ciaB and cdtC, cdtA and cdtB, and cadF in four, three, and two isolates, respectively. The C. jejuni isolates expressed the virulence genes evaluated, and the expression is gene- and isolate-dependent and varied according the temperature.

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Detecção dos genes codificantes da toxina CDT, e pesquisa de fatores que influenciam na produção de hemolisinas em amostras de Campylobacter jejuni de origem avícola

Cited by 3 publications

References 32 publications

Agents of Campylobacteriosis in Different Meat Matrices in Brazil

Agents of Campylobacteriosis in Different Meat Matrices in Brazil

Microbial Resistance Due to the Use of Antimicrobials in Livestock and Agriculture

Presence of genes associated with adhesion, invasion, and toxin production inCampylobacter jejuniisolates and effect of temperature on their expression

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