2019
DOI: 10.1139/cjm-2018-0539
|View full text |Cite
|
Sign up to set email alerts
|

Presence of genes associated with adhesion, invasion, and toxin production inCampylobacter jejuniisolates and effect of temperature on their expression

Abstract: The aims of this study were to evaluate the presence of genes associated with adhesion (cadF), invasion (ciaB), and cytotoxin production (cdtA, cdtB, and cdtC) among Campylobacter jejuni isolates from a poultry slaughterhouse and to investigate the effect of different temperatures on the expression of these virulence-associated genes. A total of 88 C. jejuni isolates from cecum, liver, chicken carcasses, chilled water, and scalding water were submitted to PCR assay for detection of virulence genes. Representat… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

4
9
0
1

Year Published

2020
2020
2023
2023

Publication Types

Select...
6
1
1

Relationship

1
7

Authors

Journals

citations
Cited by 15 publications
(14 citation statements)
references
References 59 publications
4
9
0
1
Order By: Relevance
“…Among the thermophilic Campylobacter species, C. jejuni is the species most isolated in the productive chain of broilers [41,42] and is also responsible for 90% of cases of human campylobacteriosis [6]. Our results showed that 75% (9/12) of the cecum samples were positive for C. jejuni.…”
Section: Discussionmentioning
confidence: 60%
“…Among the thermophilic Campylobacter species, C. jejuni is the species most isolated in the productive chain of broilers [41,42] and is also responsible for 90% of cases of human campylobacteriosis [6]. Our results showed that 75% (9/12) of the cecum samples were positive for C. jejuni.…”
Section: Discussionmentioning
confidence: 60%
“…We noted lack of tssL and tssM genes encoding inner membrane proteins and lack of the tssI gene encoding VgrG protein that functions as a puncturing device toward the targeted cells (Silverman et al, 2012). However, all T6SS-positive Campylobacter isolates possessed the hcp gene highlighted by Corcionivoschi et al (2015) as an indicative component of a functional T6SS in C. jejuni.…”
Section: Discussionmentioning
confidence: 88%
“…Multiple bacterial factors have been implicated in the pathogenesis of campylobacteriosis including adhesion, invasion, and production of certain toxins, which support Campylobacter to invade the host, cause disease, and evade host defense (de Oliveira et al, 2019). Identification of virulence factors involved in the pathogenesis of campylobacteriosis is crucial to better understanding the mechanisms of the infection and to identify if certain, potentially more virulent strains exist.…”
Section: Introductionmentioning
confidence: 99%
“…The intestinal life cycle of C. jejuni requires transition to an animal's gut, where it responds to changes in physiological conditions. The abundance of the CadF and FlpA proteins has been demonstrated to be responsive to host conditions, including temperature, oxygen levels, oxidative stress, and mucin (Tu et al, 2008;Hong et al, 2014;Koolman et al, 2016;Guccione et al, 2017;de Oliveira et al, 2019). For example, Hong and colleagues reported that culturing C. jejuni with porcine mucin resulted in an increase in 32 proteins and a decrease in 20 proteins compared to bacteria grown in the absence of mucin for 24 h using a label-free LC-MS/MS technique (Hong et al, 2014).…”
Section: Cadf and Flpa Regulation In Response To Intestinal Conditionsmentioning
confidence: 99%