Agents of Campylobacteriosis in Different Meat Matrices in Brazil

Abstract

We aimed to identify the prevalence of thermophilic species of Campylobacter in meats of different species available on the Brazilian commercial market and to determine the genetic diversity, antimicrobial resistance and virulence potential of the isolates. A total of 906 samples, including chicken, beef and pork carcasses and chicken and beef livers, were purchased in retail outlets, and prevalences of 18.7% (46/246), 3.62% (5/138), 10.14% (14/138), 3.62% (5/138) and 4.47% (11/132), respectively, were identified, evidencing the dissemination of genotypes in the main producing macro-regions. Of all isolates, 62.8% were classified as multidrug resistant (MDR), with resistance to amoxicillin-clavulanate (49.4%), tetracycline (51.8%) and ciprofloxacin (50.6%) and co-resistance to macrolides and fluoroquinolones (37.1%). Multivirulent profiles were identified mainly in isolates from chicken carcasses (84.8%), and the emergence of MDR/virulent strains was determined in pork isolates. All isolates except those from chicken carcasses showed a high potential for biofilm formation (71.4% luxS) and consequent persistence in industrial food processing. For chicken carcasses, the general virulence was higher in C. jejuni (54.3%), followed by C. coli (24%) and Campylobacter spp. (21.7%), and in the other meat matrices, Campylobacter spp. showed a higher prevalence of virulence (57.2%). The high rates of resistance and virulence reinforce the existence of strain selection pressure in the country, in addition to the potential risk of strains isolated not only from chicken carcasses, but also from other meat matrices.

Bacteriological quality and antimicrobial susceptibility profiles of isolates of ready-to-eat raw minced meat from hotels and restaurants in Arba Minch, Ethiopia

“…observed currently was lower than that detected in a previous study done in Bangladesh (89.1%) [ 59 ]. The percentage of MDR isolates of Campylobacter spp., found in the present study (56.7%) was higher than that previously reported from other regions of Ethiopia (20% and 14.5%) [ 41 , 64 ], but was much lower than what resulted from a couple of studies done in Brazil (62.8%) [ 65 ] and Korea (93.4%) [ 55 ]. In the case of S .…”

Prevalence, antimicrobial resistance, virulence gene profile and molecular typing of Campylobacter species isolated from poultry meat samples

“…Thus, additional studies should be conducted to explore more in‐depth the relationship between the pathogenic traits and the antimicrobial resistance in Campylobacter strains. The high distribution of virulence factors was also reported in surveys conducted in the United States (Poudel et al., 2022 ), Poland (Wieczorek et al., 2018 ), Brazil (Takeuchi et al., 2022 ), Pakistan (Melo et al., 2013 ) and China (Zhang et al., 2016 ). We found a higher distribution of virulence factors among the C. jejuni isolates than C. coli bacteria.…”

Prevalence and Characterization of Salmonella Isolated from Chickens in Anhui, China

“…According to Abd El-Aziz et al [ 51 ], there are many XDR Salmonella isolates in livestock animals exhibiting co-resistance to ciprofloxacin (CIP) and tigecycline (TIG), and this co-resistance is facilitated by the overexpression of acrAB, which enhances efflux-mediated resistance to CIP/TIG. The phenomenon of co-resistance is prevalent in MDR pathogenic bacteria isolated from animals, such as amoxicillin and tetracycline co-resistance in Escherichia coli [ 52 ], and co-resistance to macrolides and fluoroquinolones in Campylobacter [ 53 ]. Therefore, the molecular mechanism of co-resistance remains to be further studied.…”