Design, Preparation, and Performance of a Novel Bilayer Tissue‐Engineered Small‐Diameter Vascular Graft

Ran, Xiaolin; Ye, Zhiyi; Fu, Meiling; Wang, Qilong; Wu, Haide; Lin, Song; Yin, Tieying; Hu, Tingzhang; Wang, Guixue

doi:10.1002/mabi.201800189

Cited by 32 publications

(17 citation statements)

References 57 publications

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“…For TEVGs, decellularized scaffolds can be obtained from autografts, such as from the human umbilical artery [30,31] and internal mammary artery [32], or from xenografts [33]. They are seeded with cells such as stem cells and then cultured in a bioreactor.…”

Section: Decellularized Matrixmentioning

confidence: 99%

Acellular Small-Diameter Tissue-Engineered Vascular Grafts

et al. 2019

Applied Sciences

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Tissue-engineered vascular grafts (TEVGs) are considered one of the most effective means of fabricating vascular grafts. However, for small-diameter TEVGs, there are ongoing issues regarding long-term patency and limitations related to long-term in vitro culture and immune reactions. The use of acellular TEVG is a more convincing method, which can achieve in situ blood vessel regeneration and better meet clinical needs. This review focuses on the current state of acellular TEVGs based on scaffolds and gives a summary of the methodologies and in vitro/in vivo test results related to acellular TEVGs obtained in recent years. Various strategies for improving the properties of acellular TEVGs are also discussed.

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Section: Decellularized Matrixmentioning

confidence: 99%

Acellular Small-Diameter Tissue-Engineered Vascular Grafts

et al. 2019

Applied Sciences

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“…Due to the nature of tissue engineering grafts, some studies expect neo-tissue formation using materials with fast degradation rates [70,71]. In the arterial environment, these grafts tend to dilate relatively quickly.…”

Section: Discussionmentioning

confidence: 99%

Heparin-Eluting Tissue-Engineered Bioabsorbable Vascular Grafts

et al. 2021

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The creation of small-diameter tissue-engineered vascular grafts using biodegradable materials has the potential to change the quality of cardiovascular surgery in the future. The implantation of these tissue-engineered arterial grafts has yet to reach clinical application. One of the reasons for this is thrombus occlusion of the graft in the acute phase. In this paper, we first describe the causes of accelerated thrombus formation and discuss the drugs that are thought to inhibit thrombus formation. We then review the latest research on methods to locally bind the anticoagulant heparin to biodegradable materials and methods to extend the duration of sustained heparin release. We also discuss the results of studies using large animal models and the challenges that need to be overcome for future clinical applications.

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“…However, the process of decellularization can damage the extracellular matrix. To circumvent this limitation, synthetic material has been used to create a reinforced hybrid vascular graft that was used in a rat aorta interposition model ( Gong et al, 2016 ; Ran et al, 2019 ). New automated technology based on dip-spinning methodologies were proposed to manufacture constructs with native mechanical properties and cell-derived biological activities, critical for clinical graft applications ( Akentjew et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Reconstruction of the Swine Pulmonary Artery Using a Graft Engineered With Syngeneic Cardiac Pericytes

Alvino

Thomas

Ghorbel

et al. 2021

Front. Bioeng. Biotechnol.

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The neonatal heart represents an attractive source of regenerative cells. Here, we report the results of a randomized, controlled, investigator-blinded preclinical study, which assessed the safety and effectiveness of a matrix graft cellularized with cardiac pericytes (CPs) in a piglet model of pulmonary artery (PA) reconstruction. Within each of five trios formed by 4-week-old female littermate piglets, one element (the donor) was sacrificed to provide a source of CPs, while the other two elements (the graft recipients) were allowed to reach the age of 10 weeks. During this time interval, culture-expanded donor CPs were seeded onto swine small intestinal submucosa (SIS) grafts, which were then shaped into conduits and conditioned in a flow bioreactor. Control unseeded SIS conduits were subjected to the same procedure. Then, recipient piglets were randomized to surgical reconstruction of the left PA (LPA) with unseeded or CP-seeded SIS conduits. Doppler echocardiography and cardiac magnetic resonance imaging (CMRI) were performed at baseline and 4-months post-implantation. Vascular explants were examined using histology and immunohistochemistry. All animals completed the scheduled follow-up. No group difference was observed in baseline imaging data. The final Doppler assessment showed that the LPA’s blood flow velocity was similar in the treatment groups. CMRI revealed a mismatch in the average growth of the grafted LPA and contralateral branch in both treatment groups. Histology of explanted arteries demonstrated that the CP-seeded grafts had a thicker luminal cell layer, more intraparietal arterioles, and a higher expression of endothelial nitric oxide synthase (eNOS) compared with unseeded grafts. Moreover, the LPA stump adjacent to the seeded graft contained more elastin and less collagen than the unseeded control. Syngeneic CP engineering did not accomplish the primary goal of supporting the graft’s growth but was able to improve secondary outcomes, such as the luminal cellularization and intraparietal vascularization of the graft, and elastic remodeling of the recipient artery. The beneficial properties of neonatal CPs may be considered in future bioengineering applications aiming to reproduce the cellular composition of native arteries.

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Design, Preparation, and Performance of a Novel Bilayer Tissue‐Engineered Small‐Diameter Vascular Graft

Cited by 32 publications

References 57 publications

Acellular Small-Diameter Tissue-Engineered Vascular Grafts

Acellular Small-Diameter Tissue-Engineered Vascular Grafts

Heparin-Eluting Tissue-Engineered Bioabsorbable Vascular Grafts

Reconstruction of the Swine Pulmonary Artery Using a Graft Engineered With Syngeneic Cardiac Pericytes

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