Heparin-Eluting Tissue-Engineered Bioabsorbable Vascular Grafts

Matsuzaki, Yuichi; Ulziibayar, Anudari; Shoji, Tetsuo; Shinoka, Toshiharu

doi:10.3390/app11104563

Cited by 14 publications

(10 citation statements)

References 72 publications

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“…Chemical conjugation methods contain covalent and ionic bonding. 130 Zhu et al 64 covalently bonded heparin with PEG to poly(ester urethane)urea (PEUU) and resulted in no thrombosis within the lumen after 30 days in vivo.…”

Section: Strategies To Prevent Intimal Hyperplasia In Vascular Graftsmentioning

confidence: 99%

“…Methods used to impart heparin into vascular grafts include physical conjugation methods such as gas plasma, straight mixing with sulfated biopolymers, and coaxial electrospinning. Chemical conjugation methods contain covalent and ionic bonding . Zhu et al covalently bonded heparin with PEG to poly(ester urethane)urea (PEUU) and resulted in no thrombosis within the lumen after 30 days in vivo.…”

Section: Strategies To Prevent Intimal Hyperplasia In Vascular Graftsmentioning

confidence: 99%

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Review of Polymeric Biomimetic Small-Diameter Vascular Grafts to Tackle Intimal Hyperplasia

2022

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Small-diameter artificial vascular grafts (SDAVG) are used to bypass blood flow in arterial occlusive diseases such as coronary heart or peripheral arterial disease. However, SDAVGs are plagued by restenosis after a short while due to thrombosis and the thickening of the neointimal wall known as intimal hyperplasia (IH). The specific causes of IH have not yet been deduced; however, thrombosis formation due to bioincompatibility as well as a mismatch between the biomechanical properties of the SDAVG and the native artery has been attributed to its initiation. The main challenges that have been faced in fabricating SDAVGs are facilitating rapid re-endothelialization of the luminal surface of the SDAVG and replicating the complex viscoelastic behavior of the arteries. Recent strategies to combat IH formation have been mostly based on imitating the natural structure and function of the native artery (biomimicry). Thus, most recently, developed grafts contain a multilayered structure with a designated function for each layer. This paper reviews the current polymeric, biomimetic SDAVGs in preventing the formation of IH. The materials used in fabrication, challenges, and strategies employed to tackle IH are summarized and discussed, and we focus on the multilayered structure of current SDAVGs. Additionally, the future aspects in this area are pointed out for researchers to consider in their endeavor.

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Section: Strategies To Prevent Intimal Hyperplasia In Vascular Graftsmentioning

confidence: 99%

Section: Strategies To Prevent Intimal Hyperplasia In Vascular Graftsmentioning

confidence: 99%

Review of Polymeric Biomimetic Small-Diameter Vascular Grafts to Tackle Intimal Hyperplasia

2022

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“…The issue is that heparin release patterns are frequently characterized by a substantial initial burst of heparin. After which very little or nothing remains on the scaffold, so strategies to limit and control heparin release have been developed “Heparin immobilization” ( Matsuzaki et al, 2021 ). Therefore, to get better hemocompatibility after in vivo perfusion of decellularized scaffolds of swine liver, heparin immobilization technique was used by Bao et al (2015) before auxiliary transplantation.…”

Section: Passivation Methods and Anticoagulants For Revascularized Bi...mentioning

confidence: 99%

Re-Endothelialization of Decellularized Liver Scaffolds: A Step for Bioengineered Liver Transplantation

Tharwat

Larson

et al. 2022

Front. Bioeng. Biotechnol.

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Bioengineered livers (BELs) are an attractive therapeutic alternative to address the donor organ shortage for liver transplantation. The goal of BELs technology aims at replacement or regeneration of the native human liver. A variety of approaches have been proposed for tissue engineering of transplantable livers; the current review will highlight the decellularization-recellularization approach to BELs. For example, vascular patency and appropriate cell distribution and expansion are critical components in the production of successful BELs. Proper solutions to these components of BELs have challenged its development. Several strategies, such as heparin immobilization, heparin-gelatin, REDV peptide, and anti-CD31 aptamer have been developed to extend the vascular patency of revascularized bioengineered livers (rBELs). Other novel methods have been developed to enhance cell seeding of parenchymal cells and to increase graft functionality during both bench and in vivo perfusion. These enhanced methods have been associated with up to 15 days of survival in large animal (porcine) models of heterotopic transplantation but have not yet permitted extended survival after implantation of BELs in the orthotopic position. This review will highlight both the remaining challenges and the potential for clinical application of functional bioengineered grafts.

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“…Anticoagulant activity of a vascular scaffold can be obtained through the incorporation of heparin or heparin-like modifications of polymeric matrices [ 14 , 15 , 16 ]. Heparin is considered a “polypharmaceutical”; this natural polysaccharide, besides preventing clot formation, is also able to promote anti-inflammatory behavior, regulate angiogenesis, and presents anti-cancer and antiviral activity [ 15 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Preliminary Results on Heparin-Modified Double-Layered PCL and PLA-Based Scaffolds for Tissue Engineering of Small Blood Vessels

Domalik-Pyzik

Morawska-Chochół

2022

JFB

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Low-diameter blood vessels are challenging to replace with more traditional synthetic vascular grafts. Therefore, the obvious choice is to try to regenerate small veins and arteries through tissue-engineering approaches. However, the layered structure of native vessels and blood compatibility issues make this a very challenging task. The aim of this study is to create double-layered tubular scaffolds with enhanced anticoagulant properties for the tissue engineering of small blood vessels. The scaffolds were made of a polycaprolactone-based porous outer layer and a polylactide-based electrospun inner layer modified with heparin. The combination of thermally induced phase separation and electrospinning resulted in asymmetric scaffolds with improved mechanical properties. The release assay confirmed that heparin is released from the scaffolds. Additionally, anticoagulant activity was shown through APTT (activated partial thromboplastin time) assay. Interestingly, the endothelial cell culture test revealed that after 14 days of culture, HAECs (human aortic endothelial cell lines) tended to organize in chain-like structures, typical for early stages of vascular formation. In the longer culture, HAEC viability was higher for the heparin-modified scaffolds. The proposed scaffold design and composition have great potential for application in tissue engineering of small blood vessels.

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Heparin-Eluting Tissue-Engineered Bioabsorbable Vascular Grafts

Cited by 14 publications

References 72 publications

Review of Polymeric Biomimetic Small-Diameter Vascular Grafts to Tackle Intimal Hyperplasia

Review of Polymeric Biomimetic Small-Diameter Vascular Grafts to Tackle Intimal Hyperplasia

Re-Endothelialization of Decellularized Liver Scaffolds: A Step for Bioengineered Liver Transplantation

Preliminary Results on Heparin-Modified Double-Layered PCL and PLA-Based Scaffolds for Tissue Engineering of Small Blood Vessels

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