Design and in vitro investigation of nanocomposite hydrogel based in situ spray dressing for chronic wounds and synthesis of silver nanoparticles using green chemistry

Abstract

An innovative concept of spontaneous film dressing has been designed as sprayed hydrogel dressings (SHD) using a blend of polyvinyl alcohol (PVA) and sodium alginate (SA) as synthetic and natural polymeric components and, were crosslinked with boric acid and calcium chloride, respectively. Silver nanoparticles (AgNPs) are synthesized by green chemistry using Ficus benghalensis extract (FB) and were characterized by SEM and zeta sizer. FTIR spectra show polymeric interaction with AgNP s , while SEM images show outer surface of the SHD film. Equilibrium swelling and degradation in aqueous media (distilled water and buffers) are found to be dependent upon PVA/SA ratio. Polymeric combination exhibit pseudoplastic behavior with Farrow's constant >1. Uniformly distributed AgNPs (particle size 27.55 6 2.01 nm), high water retention (13 fold), and biodegradable (5 days) nature of dressing along with sustained release profile of both AgNPs and extract with concentration-dependent antimicrobial activity have been observed for 24 h. Self shaped, biodegradable, aseptic, prolong anti-oxidative, non-hemolytic, blood compatible, and hemostatic properties of SHD film appears as promising dressing for superficial wounds.

Facile Incorporation of Silver Nanoparticles into Quaternized Poly(2-(Dimethylamino)Ethyl Methacrylate) Brushes as Bifunctional Antibacterial Coatings

“…Two methods (i.e., the disc diffusion and the CFU) were used to evaluate the antibacterial activity both qualitatively and quantitatively. The disc diffusion method was performed simply by putting the samples onto the bacteria coated agar plates, and then the zone of inhibition was examined after 24 h . CFU method was performed by counting the viable bacterial colonies as described in the literature .…”

In Vivo Investigation of Soft Tissue Response of Novel Silver/Poly(Vinyl Alcohol)/ Graphene and Silver/Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Aimed for Medical Applications – The First Experience

“…Poly(vinyl alcohol) (PVA) is a synthetic polymer, which is commonly used in the preparation of biocomposites for various applications, such as wound dressings [2,3] and scaffolds for tissue engineering [4][5][6] due to its biocompatibility and non-toxicity. Due to their exceptional hydrophilicity, with many -OH groups on the side chain, PVA hydrogels are able to swell in aqueous solutions.…”

Chemical Modifications of Alginate and Its Derivatives

“…5. In another study, a novel idea of spontaneous film dressing (SHD) was reported by Kumar [65] . In vitro properties like degradation, microbe penetration assay and water absorption ability were investigated.…”