Depth profiles of hydrogen bound water molecule types and their relation to lipid and protein interaction in the human stratum corneum in vivo

Choe, ChunSik; Lademann, Jürgen; Darvin, Maxim E.

doi:10.1039/c6an01717g

Cited by 141 publications

(192 citation statements)

References 62 publications

(91 reference statements)

Supporting

Mentioning

181

Contrasting

Order By: Relevance

“…Figure A shows the ratio of weakly bound/strongly bound water molecule types in the skin, which represent the hydrogen bonding state of water molecules . It can clearly be seen that the application of both OCAs within 30 and 60 minutes did not affect the hydrogen bonding states of water in the depths 0 to 200 μm.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Sdobnov

Darvin

Schleusener

et al. 2019

Journal of Biophotonics

Self Cite

View full text Add to dashboard Cite

Confocal Raman microscopy has been used to measure depth‐dependent profiles of porcine skin ex vivo in the high wavenumber region after application of molecular optical clearing agents (OCAs). Glycerol (70%) and iohexol (100% Omnipaque [300]) water solutions were used as OCAs and topically applied to porcine ear skin for 30 and 60 minutes. Using Gaussian function–based deconvolution, the changes of hydrogen bound water molecule types have been microscopically analyzed down to the depth of 200 μm. Results show that both OCAs induced skin dehydration (reduction of total water), which is 51.3% for glycerol (60 minutes), 33.1% for glycerol (30 minutes), 8.3% for Omnipaque (60 minutes) and 4.4% for Omnipaque (30 minutes), on average for the 40 to 200 μm depths. Among the water types in the skin, the following reduction was observed in concentration of weakly bound (51.1%, 33.2%, 7.5% and 4.6%), strongly bound (50.4%, 33.0%, 7.9% and 3.4%), tightly bound (63.6%, 42.3%, 26.1% and 12.9%) and unbound (55.4%, 28.7%, 10.1% and 5.9%) water types on average for the 40 to 200 μm depths, post application of glycerol (60 minutes), glycerol (30 minutes), Omnipaque (60 minutes) and Omnipaque (30 minutes), respectively. As most concentrated in the skin, weakly and strongly bound water types are preferentially involved in the OCA‐induced water flux in the skin, and thus, are responsible for optical clearing efficiency.

show abstract

Section: Resultsmentioning

confidence: 99%

“…As far as Raman spectra of the molecular vibrations are overlaid by fluorescence of the skin, the baseline removal procedure was performed using the method proposed by Choe et al …”

Section: Methodsmentioning

confidence: 99%

Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Sdobnov

Darvin

Schleusener

et al. 2019

Journal of Biophotonics

Self Cite

View full text Add to dashboard Cite

show abstract

“…for a routine investigation of cosmetic problems. This led to an impetuous development of several in vivo methods, which allow for the assessment of skin structure, including high frequency ultrasonography 23 , optical coherence tomography (OCT) 24, 25 , confocal laser scanning microscopy (LSM) 26–28 , confocal Raman microscopy (RM) 29, 30 and multiphoton imaging 31 , including multiphoton tomography (MPT) 32 combined with coherent anti-Stokes Raman scattering (CARS) 33, 34 .…”

Section: Introductionmentioning

confidence: 99%

Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization

Shirshin

Gurfinkel

Priezzhev

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

The papillary dermis of human skin is responsible for its biomechanical properties and for supply of epidermis with chemicals. Dermis is mainly composed of structural protein molecules, including collagen and elastin, and contains blood capillaries. Connective tissue diseases, as well as cardiovascular complications have manifestations on the molecular level in the papillary dermis (e.g. alteration of collagen I and III content) and in the capillary structure. In this paper we assessed the molecular structure of internal and external regions of skin capillaries using two-photon fluorescence lifetime imaging (FLIM) of endogenous compounds. It was shown that the capillaries are characterized by a fast fluorescence decay, which is originated from red blood cells and blood plasma. Using the second harmonic generation signal, FLIM segmentation was performed, which provided for spatial localization and fluorescence decay parameters distribution of collagen I and elastin in the dermal papillae. It was demonstrated that the lifetime distribution was different for the inner area of dermal papillae around the capillary loop that was suggested to be due to collagen III. Hence, we propose a generalized approach to two-photon imaging of the papillary dermis components, which extends the capabilities of this technique in skin diagnosis.

show abstract

“…Although all textiles showed highly significant increase in relative moisture determined by a Corneometer, the increase in cotton, having the highest water permeability, is lowest. Alternative approaches to determine the hydration status of the skin could be confocal Raman microscopy, which can also be applied depth dependently and in vivo …”

Section: Discussionmentioning

confidence: 99%

Influence of polyester spacer fabric, cotton, chloroprene rubber, and silicone on microclimatic and morphologic physiologic skin parameters in vivo

Jung

Schleusener

Knorr

et al. 2019

Skin Research and Technology

Self Cite

View full text Add to dashboard Cite

Background Skin diseases can develop upon disadvantageous microclimate in relation to skin contact with textiles of supporting devices. Increased temperature, moisture, mechanical fracture, pressure, and inflammatory processes often occur mutually and enhance each other in their adverse effects. Therefore, the early prevention of skin irritations by improvement of microclimatic properties of skin in contact with supporting devices is important. Materials and Methods In this study, the microclimate under occlusion with polyester, cotton, chloroprene rubber, and silicone textiles, used for supporting devices, was analyzed by determining several characteristic physiologic skin parameters in vivo, including temperature, moisture, and transepidermal water loss (TEWL). This is achieved by comparing a miniaturized in vivo detection device with several established optical and sensory methods in vivo. Results A highly significant TEWL decrease was found after polyester, chloroprene rubber, and silicone application. The application of all materials showed highly significant decrease in skin surface temperature, with chloroprene rubber showing the lowest. Similarly, all materials showed highly significant increase in relative moisture, where the highest increase was found for chloroprene rubber and silicone and the lowest increase for cotton. The cutaneous carotenoid concentration of chloroprene rubber, silicone, and polyester decreased. A manipulation of the surface structure of the stratum corneum was recognized for all materials except for cotton by laser scanning microscopy. Conclusion The skin parameters temperature, relative moisture, antioxidant status, and TEWL can effectively characterize the microclimatic environment during occlusion with medical supporting materials. These parameters could potentially be used to develop standardized testing procedures for material evaluation.

show abstract

Depth profiles of hydrogen bound water molecule types and their relation to lipid and protein interaction in the human stratum corneum in vivo

Cited by 141 publications

References 62 publications

Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization

Influence of polyester spacer fabric, cotton, chloroprene rubber, and silicone on microclimatic and morphologic physiologic skin parameters in vivo

Contact Info

Product

Resources

About