Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Sdobnov, Anton; Darvin, Maxim E.; Schleusener, Johannes; Lademann, Jürgen; Tuchin, Valery V.

doi:10.1002/jbio.201800283

Cited by 54 publications

(48 citation statements)

References 114 publications

(166 reference statements)

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“…In this way, using the proposed equation based on the diffuse reflectance imaging method can be a potentially additional useful tool for advanced diagnostics of cancerous skin tissues. Also, additional application of optical clearing [28][29][30][31][32][33] can allow enhancing the penetration depth for LSCI as well as quality of the obtained data.…”

Section: Resultsmentioning

confidence: 99%

Combined multi-wavelength laser speckle contrast imaging and diffuse reflectance imaging for skin perfusion assessment

Sdobnov

Bykov

Попов

et al. 2019

Novel Biophotonics Techniques and Applications V

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Laser Speckle Contrast Imaging (LSCI) is a powerful low-cost method for visualization of flow, microcirculation and blood perfusion. Due to the fact that diseased and healthy tissues has different blood perfusion, LSCI can be a perspective tool for cancer diagnostics and discrimination between different types of tissues. Previously, multispectral diffuse reflectance imaging method for melanoma diagnostics has been introduced. In this work, multi-wavelength (532-, 655-and 850-nm) LSCI technique combined with hyperspectral camera and diffuse reflectance imaging method will be used for assessment of tissues with different skin perfusion properties. An in vivo experiment with occlusion in human finger was performed serving as a model of tissues with different perfusion properties. The proposed method still requires further development and improvements to become a real clinical laboratory tool for non-invasive skin cancer diagnostics.

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Section: Resultsmentioning

confidence: 99%

Combined multi-wavelength laser speckle contrast imaging and diffuse reflectance imaging for skin perfusion assessment

Sdobnov

Bykov

Попов

et al. 2019

Novel Biophotonics Techniques and Applications V

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“…The density of the tumor lesion decreases and the mobility increases. This could be associated with more free mobile water molecules in the skin as shown by Raman spectroscopic investigations, attributing different mobilities of water molecules to the different strengths of hydrogen bonds …”

Section: Discussionmentioning

confidence: 99%

Application of parelectric spectroscopy to detect skin cancer—A pilot study

Arnold‐Brüning

Blaschke

Kramer

et al. 2019

Skin Research and Technology

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Background The early detection of skin cancer is still challenging and calls for objective, fast diagnostic, and ideally non‐invasive methods in order to leave the potentially malignant tumor cells unaltered. In this paper, the parelectric spectroscopy was applied to evaluate the potential of a non‐invasive detection of basal cell carcinoma (BCC) and malignant melanoma. Materials and Methods A prototype of parelectric spectroscopy was used to investigate non‐invasively dipole density and mobility of suspicious skin lesions. The differences in investigated tissue were analyzed compared to pathohistological findings in a clinical study on 51 patients with suspected BCC and malignant melanoma. Results The non‐invasive parelectric spectroscopy could differentiate between normal skin, BCC, and melanoma but failed to distinguish between different types of skin cancer. The data were normalized to unsuspected nearby skin because the different skin locations influence dipole density and mobility. Conclusion The results of the pilot study indicate that the parelectric spectroscopy might be an additional, useful non‐invasive diagnostic procedure to distinguish between normal skin and skin cancer.

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“…Beneath the SC, in the viable epidermis and in the papillary/reticular dermis, the concentration of total water is comparable to that measured in the bottom layer of the SC [47]. Therefore, the multiplication by the same correction coefficients is also needed for these depths [35] in order to adjust protein inhomogeneity in the sampling volume, used for normalization.…”

Section: Water Concentrationmentioning

confidence: 94%

Non-invasive depth profiling of the stratum corneum in vivo using confocal Raman microscopy considering the non-homogeneous distribution of keratin

Darvin

Choe

Schleusener

et al. 2019

Biomed. Opt. Express

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Confocal Raman microscopy has a number of advantages in investigating the human stratum corneum (SC) in vivo and ex vivo. The penetration profiles of xenobiotics in the SC, as well as depth profiles of the physiological parameters of the SC, such as the concentration of water depending on the strength of hydrogen bonds, total water concentration, the hydrogen bonding state of water molecules, concentration of intercellular lipids, the lamellar and lateral packing order of intercellular lipids, the concentration of natural moisturizing factor molecules, carotenoids, and the secondary and tertiary structure properties of keratin are well investigated. To consider the depth-dependent Raman signal attenuation, in most cases a normalization procedure is needed, which uses the main SC's protein keratin-related Raman peaks, based on the assumption that keratin is homogeneously distributed in the SC. We found that this assumption is not accurate for the bottom part of the SC, where the water concentration is considerably increased, thus, reducing the presence of keratin. Our results demonstrate that the bottom part of the SC depth profile should be multiplied by 0.94 in average in order to match this non-homogeneity, which result in a decrease of the uncorrected values in these depths. The correctly normalized depth profiles of the concentration of lipids, water, natural moisturizing factor and carotenoids are presented in this work. The obtained results should be taken into consideration in future skin research using confocal Raman microscopy. IntroductionThe stratum corneum (SC), the outermost continuously renewing skin layer, provides the barrier function. It is important for the regulation of water balance and the penetration ability of xenobiotics through the skin. The SC consists of corneocytes (nuclear-free and cytoplasmic organelles-free flattened cells), embedded in the membrane-like ordered intercellular lipid matrix. The corneocytes, which consist of keratin filaments, natural moisturizing factor (NMF) molecules and water, are surrounded by a cornified envelope representing a hardpermeable tough protein/lipid polymer structure [1]. The intercellular lipids (ICL) of the lipid matrix are organized in the form of a classical membrane and their orthorhombic lateral organization represents a highly ordered and very densely packed structure, which is notpermeable for the majority of the non-destructive substances [2][3][4]. The depth distribution of the lateral organization of ICL is non-homogeneous in the SC, showing a prevalence of orthorhombic over hexagonal ordering in the intermediate SC depths measured in vitro [5,6]. The prevalence of the orthorhombic lateral organization in the SC maintains the skin barrier

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Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Cited by 54 publications

References 114 publications

Combined multi-wavelength laser speckle contrast imaging and diffuse reflectance imaging for skin perfusion assessment

Combined multi-wavelength laser speckle contrast imaging and diffuse reflectance imaging for skin perfusion assessment

Application of parelectric spectroscopy to detect skin cancer—A pilot study

Non-invasive depth profiling of the stratum corneum in vivo using confocal Raman microscopy considering the non-homogeneous distribution of keratin

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