Dental Enamel Caries (Early) Diagnosis and Mapping by Laser Raman Spectral Imaging

Applications of Raman Spectroscopy in Dentistry: Analysis of Tooth Structure

“…Raman spectroscopy helps in characterizing hydroxyapatite crystals (Ca 10 (PO 4 ) 6 (OH) 2 (3,36,39). Several studies strongly recommend Raman spectroscopy as an effective and relatively easy tool for early diagnosis of dental caries (32,(40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50) because during the caries process the inorganic matter of the tooth is gradually replaced by organic matter, which shows stronger organic peaks than normally seen. These changes are characterized by studying material compositions by exciting chemical bonds in the specimen (51).…”

“…These changes are characterized by studying material compositions by exciting chemical bonds in the specimen (51). Bulatov et al (48) studied normal and carious teeth and found that the carious process affects the phosphate and carbonate phase (Figure 4) of enamel and dentin, which represent the mineral phase of tooth structure.…”

Application of raman spectroscopy method to the diagnostics of caries development

“…In [6,7,8], using the method of Raman spectroscopy determined that the difference between healthy and carious areas of enamel is well observed at wave number 959 cm -1 , as well as the study of various intensities PO4 3-oscillations (1043, 590, and 431 cm -1 ) revealed a consistent change in the intensity of the spectra of carious areas compared to healthy. The spectral changes associated with demineralization by altering the elemental composition of the enamel.…”

Biomedical Applications of Raman Imaging

“…Raman microspectroscopy has also proved useful in visualizing mineral/protein distributions and stress fields across dentinoenamel interfaces [139][140][141][142]. For example, confocal Raman system was used to image the microscopic stress fields across a dentinoenamel junction (DEJ) of a sectioned cattle tooth under increasing compressive loads [141].…”