Applications of Raman Spectroscopy in Dentistry: Analysis of Tooth Structure

Abstract

Tooth enamel is the most mineralized tissue in the human body, and in this

Application of raman spectroscopy method to the diagnostics of caries development

“…It is well known that caries is the most widespread and unpredictable problem in stomatology [1,2]. At the first stages it is symptomless, but later the progressing caries can lead to the development of pulpit and finally to the tooth extraction and the consequent patient's aesthetic problem.…”

Ex vivo detection and characterization of remineralized carious dentin, by nanoindentation and single point Raman spectroscopy, after amalgam restoration

“…This higher presence of minerals complied with a drop in the bacterial metabolic activity and organic content detected at the carious tissue after removal of both kind of amalgam restorations, as was reflected in Table 3 and Fig. 2C, by the decrease in fluoridated apatite (peak at 575 cm -1 ) [32] . The lack of augmentation (in caries-affected dentin treated with Zn-free amalgams) or the discrete growth (in caries-affected dentin treated with Zn-containing amalgams) of 579/959, 1043/959 and 1069/959 ( Table 3) makes sure that the caries-affected tissue is decreasing as result of dentin remineralization [5] .…”

“…Peak at 1620 cm -1 is attributed to the amide I feature of dentin and hydrated gel-like collagen. The frequency of this feature, which corresponds to the spectral peak that is assigned to the Y8a tyrosine side chain of solution-phase collagen [32] , augmented in both untreated (~1.49 fold) and specimens treated with Zn-containing amalgams (~1.04 fold), when sound and caries-effected dentin were compared (Table 4). Therefore, an improvement of the structural treated dentin which is typically recognized by specific helical 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 F o r P e e r R e v i e w polypeptide back bone torsion angles and specific main chain hydrogen bond pairings, is expected.…”

“…We speculate that zinc may have acted as crystal growth inhibitor facilitating amorphous calcium phosphate stabilization, intrafibrillar mineralization of collagen, and the ensuing increase of mechanical properties [59,60] ( Table 2). It may be observed that in the v 1 spectral region (1000-900 cm -1 ), Raman spectra always contain detailed band peaks with a relative narrow line width (<12 cm -1 full width at half maximum for the major peaks) [32] , in the untreated samples. It also may be observed that these bands are weaker in intensity and wider after treating the caries-affected dentin with both Zn-free (Fig.…”

“…3. Changes in fluoridated apatite (575 cm -1 ) reflect bacterial metabolic activity and organic content [32] . 4.…”