Dendrobine attenuates gestational diabetes mellitus in mice by inhibiting Th17 cells

Abstract

Gestational diabetes mellitus (GDM) is a common disease in pregnant women with a prevalence from 1.4% to 14% based on the population studied. The number of patients with GDM is increasing annually, and yet, the treatment for GDM remains limited. In this study, we aimed to explore the potential of dendrobine against GDM. For this purpose, a genetic mouse model of GDM was established. GDM mice were orally administered with 20 mg/kg dendrobine daily from the beginning of pregnancy to delivery. It was found that dendrobine significantly ameliorated the symptoms of GDM, as evidenced by reduced maternal body‐weight and blood glucose levels, as well as increased insulin levels and insulin sensitivity in GDM mice. Dendrobine also remarkably attenuated the impairments to offspring, such as decreased birth‐weight and birth size. Moreover, dendrobine reduced the secretion of inflammatory cytokines by T helper 17 (Th17) cells, including interleukin‐1β, interleukin‐6, tumour necrosis factor‐α and interleukin‐17. Furthermore, we found that dendrobine also reduced the population of Th17 cells in GDM mice. In conclusion, dendrobine could effectively alleviate GDM in mice and might serve as a potential therapeutic drug candidate for GDM patients.

Dendrobine suppresses endoplasmic reticulum stress-induced apoptosis through upregulating microRNA miR-381-3p to decrease caspase-4

“…The appropriate concentration of dendrobine enhanced the proliferation and promoted the apoptosis of HUVECs exposed to TM, accompanied by a marked decrease in the levels of GRP78, Ire1, CHOP and caspase-3. Dendrobine was reported to reduce inflammation in gestational diabetes mellitus (GDM) and produces a protective effect on ameliorating the symptoms of GDM and isoniazid – and rifampicin-induced liver injury [ 29 , 30 ]. In addition, it has a potential anti-tumor effect on non-small cell lung cancer [ 16 ].…”

“…Another study reported that there was a direct negative association between the miR-381-3p levels and the sensitivity to apoptosis; the ectopic expression of miR-381-3p exerted an inhibitory effect on the activation of caspase-3 [ 15 ]. Dendrobine has been shown to have the ability to lower glucose and lipid levels, exert antitumor effects, reduce inflammation and apoptosis, and to attenuate cell senescence [ 16 , 17 ]. However, the underlying molecular mechanisms remain unclear.…”

Effects of the Endophytic Fungus MF23 on Dendrobium nobile Lindl. in an Artificial Primary Environment

“…Many compounds have been extracted and isolated from the Dendrobium species, including alkaloids, , polysaccharides, , phenanthrenes, phenolics, , and bibenzyls. , Dendrobine, an index component of D. nobile, has anti-tumor, hypoglycemic, anti-flu, and other pharmacological activities…”

“…3 Many compounds have been extracted and isolated from the Dendrobium species, including alkaloids, 4,5 polysaccharides, 6,7 phenanthrenes, 8 phenolics, 9,10 and bibenzyls. 11,12 Dendrobine, an index component of D. nobile, has anti-tumor, 13 hypoglycemic, 14 anti-flu, 15 and other pharmacological activities. 16 The growth of Orchidaceae is highly affected by mycorrhizal fungi, and it has been reported that fungi can promote the germination of orchid species.…”

Dendrobine Inhibits γ-Irradiation-Induced Cancer Cell Migration, Invasion and Metastasis in Non-Small Cell Lung Cancer Cells

“…Moreover, recent biological studies have demonstrated that dendrobine possesses an antiviral activity against influenza A viruses during the early stage of the viral replication cycle [20] and hepatoprotective effects against carbon tetrachlorideinduced mouse liver injury by activating the antioxidant nuclear factor erythroid 2-related factor 2 (Nrf2) pathway [19]. Furthermore, dendrobine alleviates gestational diabetes mellitus symptoms and inflammation in mice via T helper 17 (Th17) cells [21] and enhances the anticancer activity of cisplatin against human NSCLC A549 cells in vitro and in vivo [22]. However, the effects of dendrobine against IR-induced cancer cell migration, invasion, and metastasis, as well as its molecular mechanisms, have not yet been investigated.…”