Dendrobine suppresses endoplasmic reticulum stress-induced apoptosis through upregulating microRNA miR-381-3p to decrease caspase-4

Abstract

Dendrobine has been reported to reduce blood lipid levels and apoptosis. The present study was designed to observe the effect of dendrobine in a model of ERS using vascular endothelial cells and to reveal the biological mechanisms and pathways responsible for the therapeutic effects of dendrobine on AS. Human umbilical vein endothelial cells (HUVECs) were pre-treated with various concentrations of dendrobine, followed by treatment with tunicamycin (TM) for the establishment of the cell models of ERS. The proliferation and apoptosis of HUVECs were detected by bromodeoxyuridine staining and flow cytometry, respectively. The target binding association was verified through dual luciferase reporter assay. It was found that TM treatment resulted in a low expression of miR-381-3p. Dendrobine treatment not only promoted the proliferation, but also inhibited the apoptosis of HUVECs induced by TM. The reduced expression of 78-kDa glucoseregulated protein, inositol-requiring enzyme 1, caspase-4, C/EBP homologous protein and caspase-3 was also observed following treatment with dendrobine. Dendrobine reduced the apoptosis of endothelial cells in the model of ERS by increasing miR-381-3p expression, and partially restored the cell proliferation level. This effect was significantly reduced after the expression of miR-381-3p was blocked. On the whole, the present study demonstrated that dendrobine upregulated miR-381-3p expression to inhibit apoptosis induced by ERS in HUVECs and this process was found to be mediated by caspase-4. The findings of the present study may provide new insight into the causes of endothelial cell apoptosis during AS and reveal the potent therapeutic effects of dendrobine in AS.

Dendrobine modulates autophagy to alleviate ox‐LDL‐induced oxidative stress and senescence in HUVECs

“…Molecular mechanisms Our previous study reveals the mechanism of dendrobine involved in AS by which it protects umbilical vein endothelial cells from endoplasmic reticulum stress-induced injury (J. Meng et al, 2021).…”

The microRNA-381(miR-381)/Spindlin1(SPIN1) axis contributes to cell proliferation and invasion of colorectal cancer cells by regulating the Wnt/β-catenin pathway

“…Several studies have suggested that miR-381 exerts its critical role in physical and pathological processes, including cell growth, apoptosis and tumorigenesis [ 35–39 ]. For example, dendrobine suppressed the endoplasmic reticulum stress-induced apoptosis by increasing miR-381-3p expression in endothelial cells [ 38 ]. Previously, miR-381 was reported to suppress cell proliferation and metastasis by targeting the BM1 and Rho/ROCK axis in bladder cancer [ 40 ].…”

microRNA-301a-3p is a potential biomarker in venous ulcers vein and gets involved in endothelial cell dysfunction

“…The membranes were flushed three times. The enhanced chemiluminescence (ECL) color developing agent (Millipore, Bedford, MA, USA) was deployed for exposure, and a membrane scanner was adopted for imaging [ 32 ].…”

“…Then, HUVECs were transfected with pMIR-IGF1-WT, pMIR-IGF1-MT, miR-301a-3p mimics, and miR-NC with the use of lipofectamine® 3000 for 48 hours. The Promega dual luciferase reporter system was introduced to analyze the relevant luciferase activity [ 32 ].…”