Effects of the Endophytic Fungus MF23 on Dendrobium nobile Lindl. in an Artificial Primary Environment

Abstract

The quality of Dendrobium nobile Lindl. is related to its endophytic fungi. It has been reported that the mycorrhizal fungus MF23 helps to increase the content of dendrobine in Dendrobium , but few studies have explained the mechanism underlying this phenomenon. In a previous study, we verified the mechanism of symbiosis between MF23 and D. nobile on agar medium. The research carried out in this study on bark medium, similar to the natural environment, is of great importance because of its benefits for wide application. We found a significant effect, especially in the later period of cultivation, in which the highest dendrobine content in the experimental group was 0.147%, which is equivalent to 2.88 times that of the control group, and suggesting that MF23 promoted D. nobile in the natural environment, which verifies the application of the technique in field conditions. This result also implied that post-modification enzyme genes might play an important role in stimulating the biosynthesis of dendrobine.

Exogenous and Endophytic Fungal Communities of Dendrobium nobile Lindl. across Different Habitats and Their Enhancement of Host Plants’ Dendrobine Content and Biomass Accumulation

“…For example, the D. nobile mycorrhizal fungus Mycena sp. MF23 participates in dendrobine biosynthesis by regulating postmodification enzyme (cytochrome P450, aminotransferase and methyltransferase) gene expression. , Sarsaiya et al (2020) reported that D. nobile endophytic fungus T. longibrachiatum MD33 could produce the same dendrobine as the host . They also found that the MVA pathway enzyme-coding genes (hydroxymethylglutaryl-CoA synthase, farnesyl diphosphate synthase and mevalonate kinase) might participate in sesquiterpene alkaloid metabolism in T. longibrachiatum isolated from D. nobile .…”

Picrotoxane sesquiterpenoids: chemistry, chemo- and bio-syntheses and biological activities

“…[202][203][204] The dendrobine content in the stems of D. nobile increased aer infection with mycorrhizal fungus MF23 (Mycena sp.). 205 Transcriptome analysis indicated that the expressions of AACT, MVD, PMK, and TPS21 were signicantly up-regulated, which could be related to the biosynthesis of dendrobine. 206 A 3hydroxy-3-methylglutaryl-CoA reductase (HMGR) gene, named DnHMGR2 (GenBank accession no.…”

Dendrobine biosynthesis in Dendrobium nobile in four different habitats is affected by the variations in the endophytic fungal community

“…For example, 3-hydroxy-3-methylglutaryl is present only in the samples from Yunnan ( Wang et al, 2021 ). Moreover, many factors such as light intensity, water content, and microorganisms influence the abundance of chemical constituents in D. nobile ( Li et al, 2017 ; Chen X. Y. et al, 2021 ). Hence, to better control the quality of D. nobile in planting, elucidating the effect of environmental factors on the synthesis of metabolites is necessary.…”

“…The production and accumulation of dendrobine, an important index component and an active component of D. nobile , have been extensively studied. A study proved that the dominant endophytic fungi are closely related to dendrobine accumulation in D. nobile ( Chen X. Y. et al, 2021 ). Another study reported that the dendrobine content in the seedling supplying with endophytic fungi was significantly increased along with an improved growth state ( Yan et al, 2016 ).…”

“…Another study reported that the dendrobine content in the seedling supplying with endophytic fungi was significantly increased along with an improved growth state ( Yan et al, 2016 ). The endophytic mycorrhiza fungal strain MF 23 was reported to help in increasing the dendrobine content in Dendrobium ( Chen X. Y. et al, 2021 ). Some endophytic fungi such as Trichoderma longibrachiatum ( Sarsaiya et al, 2020 ) isolated from D. nobile were reported to synthesize dendrobine.…”