Dendrobine biosynthesis in Dendrobium nobile in four different habitats is affected by the variations in the endophytic fungal community

Li, Lin; Liu, Chaobo; Wen, Weie; Li, Qingqing; Pan, Tian-Tian; Li, Zhaogao; Qian, Gang; He, Yuqi; Xu, Delin

doi:10.3389/fmicb.2022.981070

Cited by 10 publications

(5 citation statements)

References 35 publications

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“…ex Andrews [16], while 2429 sequences were obtained from asymptomatic stems of Dendrobium nobile Lindl. [30].…”

Section: Discussionmentioning

confidence: 99%

“…Fungi of the phylum Ascomycota were dominant in leaves, pseudobulbs, and roots of G. skinneri, and have also been reported as abundant in the endophytic communities of Dendrobium nobile and Vanilla planifolia [16,30]. Ascomycetes also form part of the fungal communities of the bark of coffee trees (Coffea arabica L. and Coffea canephora L.; Rubiaceae), trees of the genus Inga (Fabaceae) in agroforestry systems, Quercus yiwuensis C.C.Huang ex Y.C.Hsu and H.W.Jen (Fagaceae), and Pistacia weinmannifolia J.Poiss.…”

Section: Discussionmentioning

confidence: 99%

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Community Richness and Diversity of Endophytic Fungi Associated with the Orchid Guarianthe skinneri Infested with “Black Blotch” in the Soconusco Region, Chiapas, Mexico

et al. 2023

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Orchids coexist with a diversity of endophytic fungi within their roots and other parts of the plant. These are presumed to contribute to nutrition, and may protect the plants against pathogens and herbivores; however, some may be latent pathogens and/or bring no benefit to the plant. Guarianthe skinneri is an epiphytic Central American threatened orchid used as an ornamental plant and in the rituals and celebrations of many communities. However, in the Soconusco region (Chiapas, Mexico), the pseudobulbs of mature plants are affected by the Lasiodiplodia theobromae fungus, causing the disease “black blotch”. We evaluated and compared the diversity of the endophytic fungal community within the leaves, pseudobulbs and roots of mature plants in two conditions, asymptomatic and symptomatic. Thirty samples from each condition and tissue were amplified with ITS and sequenced by Illumina MiSeq. Sequences were obtained and analyzed to determine taxonomic assignment and functionality with FUNGuild, obtaining 1857 amplicon sequence variants (ASVs). Alpha diversity was similar between plant conditions. In symptomatic plants, significant differences were found between the three types of tissue. According to the FUNGuild functionality analysis, 368 ASVs were determined to be endophytic fungi. The tissues of G. skinneri plants are reservoirs of fungal endophytes that should be considered for further exploration for research and conservation purposes.

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“…ex Andrews [16], while 2429 sequences were obtained from asymptomatic stems of Dendrobium nobile Lindl. [30].…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Community Richness and Diversity of Endophytic Fungi Associated with the Orchid Guarianthe skinneri Infested with “Black Blotch” in the Soconusco Region, Chiapas, Mexico

et al. 2023

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“…The ITS2 region of the fungi was amplified with slightly modified versions of the primers ITS1FI2 (5′-GTGARTCATCGAATCTTTG-3′) and ITS2 (5′-TCCTCCGCTTATTGATATGC-3′). Amplification, purification and sequencing library construction were performed as described in ( Li L. et al, 2022 ). The libraries were sequenced on the NovaSeq PE250 platform.…”

Section: Methodsmentioning

confidence: 99%

Fatty acid metabolites of Dendrobium nobile were positively correlated with representative endophytic fungi at altitude

Zhao¹,

Qi²,

Tan³

et al. 2023

Front. Microbiol.

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IntroductionAltitude, as a comprehensive ecological factor, regulates the growth and development of plants and microbial distribution. Dendrobium nobile (D. nobile) planted in habitats at different elevations in Chishui city, also shows metabolic differences and endophytes diversity. What is the triangular relationship between altitude, endophytes, and metabolites?MethodsIn this study, the diversity and species of endophytic fungi were tested by ITS sequencing and metabolic differences in plants were tested by UPLC–ESI–MS/MS. Elevation regulated the colonization of plant endophytic fungal species and fatty acid metabolites in D. nobile.ResultsThe results indicate that and high altitude was better for the accumulation of fatty acid metabolites. Therefore, the high-altitude characteristic endophytic floras were screened, and the correlation with fatty acid metabolites of plants was built. The colonization of T. rubrigenum, P. Incertae sedis unclassified, Phoma. cf. nebulosa JZG 2008 and Basidiomycota unclassified showed a significantly positive correlation with fatty acid metabolites, especially 18-carbon-chain fatty acids, such as (6Z,9Z,12Z)-octadeca-6,9,12-trienoic acid, 3,7,11,15-tetramethyl-12-oxohexadeca-2,4-dienoic acid and Octadec-9-en-12-ynoic acid. What is more fascinating is these fatty acids are the essential substrates of plant hormones.DiscussionConsequently, it was speculated that the D. nobile- colonizing endophytic fungi stimulated or upregulated the synthesis of fatty acid metabolites and even some plant hormones, thus affecting the metabolism and development of D. nobile.

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“…To date, the application of multiomic technology in studies on secondary metabolism to determine the interactions between plants and endophytes is becoming more widespread [ 128 , 131 ]. In research, multomics generally takes the form of metaomics, which mainly includes metagenomics, macrotranscriptomics, and macroproteomics.…”

Section: Progress In Research On Endophytes Acting On Secondary Metab...mentioning

confidence: 99%

Functional Endophytes Regulating Plant Secondary Metabolism: Current Status, Prospects and Applications

Xiong

Wen

et al. 2023

IJMS

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Endophytes, which are widely found in host plants and have no harmful effects, are a vital biological resource. Plant endophytes promote plant growth and enhance plants’ resistance to diseases, pests, and environmental stresses. In addition, they enhance the synthesis of important secondary metabolites in plants and improve the potential applicability of plants in agriculture, medicine, food, and horticulture. In this review, we summarize the recent progress in understanding the interaction between endophytes and plants and summarize the construction of synthetic microbial communities (SynComs) and metaomics analysis of the interaction between endophytes and plants. The application and development prospects of endophytes in agriculture, medicine, and other industries are also discussed to provide a reference for further study of the interaction between endophytes and plants and further development and utilization of endophytes.

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Dendrobine biosynthesis in Dendrobium nobile in four different habitats is affected by the variations in the endophytic fungal community

Cited by 10 publications

References 35 publications

Community Richness and Diversity of Endophytic Fungi Associated with the Orchid Guarianthe skinneri Infested with “Black Blotch” in the Soconusco Region, Chiapas, Mexico

Community Richness and Diversity of Endophytic Fungi Associated with the Orchid Guarianthe skinneri Infested with “Black Blotch” in the Soconusco Region, Chiapas, Mexico

Fatty acid metabolites of Dendrobium nobile were positively correlated with representative endophytic fungi at altitude

Functional Endophytes Regulating Plant Secondary Metabolism: Current Status, Prospects and Applications

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