Dendrobine Inhibits γ-Irradiation-Induced Cancer Cell Migration, Invasion and Metastasis in Non-Small Cell Lung Cancer Cells

Abstract

The use of ionizing radiation (IR) during radiotherapy can induce malignant effects, such as metastasis, which contribute to poor prognoses in lung cancer patients. Here, we explored the ability of dendrobine, a plant-derived alkaloid from Dendrobium nobile, to improve the efficacy of radiotherapy in non-small cell lung cancer (NSCLC). We employed Western blotting, quantitative real-time (qRT)-PCR, transwell migration assays, and wound-healing assays to determine the effects of dendrobine on the migration and invasion of A549 lung cancer cells in vitro. Dendrobine (5 mm) inhibited γ-irradiation-induced migration and invasion of A549 cells by suppressing sulfatase2 (SULF2) expression, thus inhibiting IR-induced signaling. To investigate the inhibitory effects of dendrobine in vivo, we established a mouse model of IR-induced metastasis by injecting BALB/c nude mice with γ-irradiated A549 cells via the tail vein. As expected, injection with γ-irradiated cells increased the number of pulmonary metastatic nodules in mice (0 Gy/DPBS, 9.8 ± 1.77; 2 Gy/DPBS, 20.87 ± 1.42), which was significantly reduced with dendrobine treatment (2 Gy/Dendrobine, 10.87 ± 0.71), by prevention of IR-induced signaling. Together, these findings demonstrate that dendrobine exerts inhibitory effects against γ-irradiation-induced invasion and metastasis in NSCLC cells in vitro and in vivo at non cytotoxic concentrations. Thus, dendrobine could serve as a therapeutic enhancer to overcome the malignant effects of radiation therapy in patients with NSCLC.

Effects of the epiphytic patterns on endophytes and metabolites of Dendrobium nobile Lindl

“…Flavonoid and phenolic acid components accounted for 37.8% of the annotated compounds, as shown in Figure 1 . The study showed that the flavonoid and phenolic acid were important active components for anti-inflammatory ( Ci et al., 2020 ; Kim et al., 2021 ; Yuan et al., 2022 ; Li et al., 2022d ), antioxidant, and hypoglycemic effects in Dendrobium. In addition, other alkaloids have been annotated in D. nobile , including sesquiterpene alkaloids, pyridine alkaloids, pyrrole alkaloids, plumerane, isoquinoline alkaloids, phenalkamines, and other alkaloids, which were the important basis for the medicinal substances and nutritional quality of D. nobile .…”

Antimetastatic activity of seongsanamide B in γ-irradiated human lung cancer

“…We then performed metastasis animal study using the IR-induced metastasis model established in a previous study [ 29 ] to investigate whether seongsanamide B suppressed IR-induced metastasis in vivo ( Fig. 5 D).…”

Effect of superfine-grinding on the physicochemical and antioxidant properties of Dendrobium nobile powders

“…Studies have shown that Dendrobium nobile mainly contains compounds such as dendrobine, polysaccharides, flavonoids and phenols, and dendrobine is the signature bioactive component, which is considered as one of the criteria for evaluating the quality and has the effects of improving gestational diabetes (Feng et al, 2021), antiviral (Li et al, 2017), neuroprotection (Li et al, 2022) and cancer inhibition (Kim et al, 2021). Polysaccharides isolated from Dendrobium have been found to have various biological functions such as immune modulation (Wang et al, 2010d) improvement of cerebral ischemia (Liu et al, 2021), anti-tumour (Luo & Fan, 2011), and hypoglycaemia (Pan et al, 2014).…”