Dehydration of Dormant Apple Buds at Different Stages of Cold Acclimation to Induce Cryopreservability in Different Cultivars

Tyler, N. J.; Stushnoff, Cecil

doi:10.4141/cjps88-145

Cited by 38 publications

(25 citation statements)

References 7 publications

(4 reference statements)

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“…This is reasonable since MC [ 30 % would negatively impact survival. Tyler and Stushnoff (1988b) investigated the effect of dehydration depending on the physiological stage of the material. When cold acclimated buds were processed during winter time, all apple cultivars became resistant to moderate dehydration stress.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of winter-dormant apple buds: establishment of a duplicate collection of Malus germplasm

Höfer

2015

Plant Cell Tiss Organ Cult

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The clonal wild-species Malus collection at the Julius Kühn-Institute, Institute for Breeding Research on Fruit Crops, Germany, comprises 507 accessions of 46 species and represents one of the largest collections in Europe. Cryopreservation was applied to 77 accessions of 31 species and hybrids in the Malus collection using a winter vegetative-bud method. No significant differences were observed in the recovery of the two controls, untreated buds and desiccated buds, however, cryopreservation significantly decreased bud recovery, independent of the rootstock used for chip budding. Data from this investigation showed an effect on recovery of year and genotype between and within species. Of the 77 accessions, 62 had living buds, and recovery was [40 % for 37 and these averaged 68.5 % recovery. The initial moisture content, dehydration time, and morphology of the buds had no significant influence on recovery. The higher percentage of recovery in one experimental year was possibly the result of secondary buds that grew despite lethal injury to the primary meristems. Adaptations to the general protocol and the handling details were successful, and the cryopreservation of dormant buds will now be part of the conservation strategy for Malus genetic resources in Germany.Cryopreservation will be a part of the conservation strategy of Malus genetic resources in Germany. Effect of moisture content, dehydration time, morphology of buds, rootstock and genotype are discussed.

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Section: Discussionmentioning

confidence: 99%

Cryopreservation of winter-dormant apple buds: establishment of a duplicate collection of Malus germplasm

Höfer

2015

Plant Cell Tiss Organ Cult

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“…Most of the water in the primordial shoot freezes out through the crown (medullary bridge) and consequently it is the crown that plays the major role in freezing avoidance in the primordial shoots of conifer buds [12,13]. Tyler & Stusnoff [18] used dormant vegetative apple buds in their cryoexperiments, dehydrating them by keeping them at -4°C for various lengths of time and then cooling them slowly to -30°C. The samples were kept at -30°C for one hour before immersion in liquid nitrogen.…”

Section: Discussionmentioning

confidence: 99%

“…The survival of the frozen buds decreased markedly (down to 7-27%) towards to the end of April. The degree of success varies with the physiological state of the buds [18] and the amount of water (Table 2). For these reasons, freeze preservation of vegetative buds cannot be done in May.…”

Section: Discussionmentioning

confidence: 99%

“…For these reasons, freeze preservation of vegetative buds cannot be done in May. The success of the freeze-preservation of apple buds was similarly dependent on the month during which the experiments were carried out, and in part upon the quantity of liquid water at the temperature to which the tissue was cooled prior to immersion in liquid nitrogen [18]. It was also noticed [10] that in winter prefreezing temperatures below -15°C were sufficient to enable willow twigs to withstand liquid nitrogen, while in early spring and in early winter prefreezing temperatures below -30°C were required.…”

Section: Discussionmentioning

confidence: 99%

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Freeze-preservation of buds from Scots pine trees

Kuoksa

Hohtola

1991

Plant Cell Tiss Organ Cult

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A procedure has been developed for freeze-preservation of buds of the Scots pine (Pinus sylvestris L.). Instead of liquid nitrogen, cold storage in -80°C was used. The partly dormant material used in the experiments was obtained directly from a natural stand in Northern Finland and no preffeezing or cryoprotectants for preconditioning were used. Cooling velocity was l°C/min up to a terminal freezing temperature of -39°C, after which the buds were immersed in liquid nitrogen at -196°C for 10 minutes. The material was then transferred to a deepfreezer at -80°C and stored up to 6 months. After rapid thawing, the buds were sterilized and their viability was tested by FDA staining and by culturing meristems on 1/2 MS medium for at least two weeks. All the freezing experiments were performed during March and April. The best survival of buds (90-100%) was achieved at the beginning of April, after which a pronounced decline in survival occurred obviously due to a rise in the water content of the buds.

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“…Cryopreservation of temperate woody species was started in Japan when Sakai (1960) demonstrated that winter hardy twigs of poplar and willow survived slow cooling and then immersion in liquid nitrogen. Since then this method has been applied and developed further for preservation of dormant buds of fruit species (Sakai and Nishiyama 1978, Tyler and Stushnoff 1988, Towill et al 2004, Toldam-Andersen et al 2007, Towill and Ellis 2008. In Finland, studies have been carried out to develop cryopreservation techniques for forest tree breeding materials since 1990's and the method for cryopreservation of dormant buds together with recovery through in vitro culture has been introduced.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation techniques and their application in vegetatively propagated crop plants in Finland

Nukari¹,

Uosukainen²,

Rokka³

2008

AFSci

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Cryopreservation protocols have been introduced as techniques for germplasm preservation of vegetatively propagated horticultural and staple food crops. In Finland, cryopreservation has been studied since 1990's, beginning with cryopreservation of forest tree breeding material and since 2004 on cryopreservation of genetic resources of horticultural plants and potato. Priority was given to cryopreservation of raspberry (Rubus ideaus L.), strawberry (Fragaria x ananassa Duch.) and potato (Solanum tuberosum L.) and the possibility to use cryotherapy in eradication of raspberry bushy dwarf virus (RBDV) from in vitro cultures were studied on raspberry. Modified droplet vitrification cryopreservation protocols were designed for raspberry and strawberry and cryotherapy combined with thermotherapy was proven to be a successful application to eliminate RBDV from infected raspberries. Cryotherapy method can be applied for a large scale elimination of viruses from plant germplasm and from candidate nuclear stock in a certified plant production scheme. Routine use of cryotechniques in germplasm preservation of vegetatively propagated horticultural plants was started. Besides for long term germplasm preservation, cryopreservation techniques can be applied also for maintenance of mother stocks in certified plant production schemes and in commercial plant production. Cryopreservation of potato shoot tips needs additional detailed research to obtain sufficient recovery and regrowth rates.

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Dehydration of Dormant Apple Buds at Different Stages of Cold Acclimation to Induce Cryopreservability in Different Cultivars

Cited by 38 publications

References 7 publications

Cryopreservation of winter-dormant apple buds: establishment of a duplicate collection of Malus germplasm

Cryopreservation of winter-dormant apple buds: establishment of a duplicate collection of Malus germplasm

Freeze-preservation of buds from Scots pine trees

Cryopreservation techniques and their application in vegetatively propagated crop plants in Finland

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