Cryopreservation of winter-dormant apple buds: establishment of a duplicate collection of Malus germplasm

Abstract

The clonal wild-species Malus collection at the Julius Kühn-Institute, Institute for Breeding Research on Fruit Crops, Germany, comprises 507 accessions of 46 species and represents one of the largest collections in Europe. Cryopreservation was applied to 77 accessions of 31 species and hybrids in the Malus collection using a winter vegetative-bud method. No significant differences were observed in the recovery of the two controls, untreated buds and desiccated buds, however, cryopreservation significantly decreased bud recovery, independent of the rootstock used for chip budding. Data from this investigation showed an effect on recovery of year and genotype between and within species. Of the 77 accessions, 62 had living buds, and recovery was [40 % for 37 and these averaged 68.5 % recovery. The initial moisture content, dehydration time, and morphology of the buds had no significant influence on recovery. The higher percentage of recovery in one experimental year was possibly the result of secondary buds that grew despite lethal injury to the primary meristems. Adaptations to the general protocol and the handling details were successful, and the cryopreservation of dormant buds will now be part of the conservation strategy for Malus genetic resources in Germany.Cryopreservation will be a part of the conservation strategy of Malus genetic resources in Germany. Effect of moisture content, dehydration time, morphology of buds, rootstock and genotype are discussed.

Cryopreservation of Ribes nigrum (L.) dormant buds: recovery via in vitro culture to the field

“…This assumption is supported by results of a previous study with Malus, as the buds with the smallest volumes had the highest survival of the primary buds when grafted following cryopreservation . Moreover, the recovery of Malus buds can be attributable to a secondary bud outgrowth Höfer 2015). In the case of cv.…”

“…This protocol is generally used with Malus (e.g. Volk et al 2008;Grout et al 2011;Lambardi et al 2011;Höfer 2015;Höfer and Hanke 2017) and also applied for Salix (Towill and Widrlechner 2004;Jenderek et al 2014) Fraxinus (Volk et al 2009) Juglans, Prunus, (Jenderek et al 2014) and Vaccinium . Protocols where recovery of buds is done via in vitro culture have been developed e.g.…”

Challenges and Prospects for the Conservation of Crop Genetic Resources in Field Genebanks, in In Vitro Collections and/or in Liquid Nitrogen

“…Additionally, pests and diseases can threaten the material, especially the less adapted or susceptible accessions. One of the important apple diseases, fire blight, caused by the bacterium Erwinia amylovora, can severely damage or even eradicate susceptible apple accession [33,34]. In 2013, the Allium field collection at IPK was challenged with a serious infestation of larval stage click beetles (Elateridae), also known as wireworms.…”

An Integrated Approach to Creating Scab-Resistant Apple: Phytopathological Testing and Marker-Assisted Selection

“…Криоконсервация спящих почек используется в основном для древесных плодовых культур умеренного климата, в частности, яблони (Forsline et al, 1999;Towill et al, 2004;Volk et al, 2008;Lambardi et al, 2009;Höfer, 2015). Однако для древесных тропических культур данный метод не подходит.…”