An Integrated Approach to Creating Scab-Resistant Apple: Phytopathological Testing and Marker-Assisted Selection

Супрун, И. И.; Насонов, А. И.; Lоbodina, Elena Vadimovna; Володина, Елена

doi:10.30901/2658-6266-2018-1-25-33

Cited by 3 publications

(2 citation statements)

References 77 publications

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“…The hybrids were obtained earlier as part of a markerassisted breeding program aimed at the development of apple scabresistant varieties with improved fruit quality characteristics. The presence of the scab resistance gene Rvi6 (Suprun et al, 2018), as well as breedingvaluable alleles of the Md-ACS1 gene, was previously determined by DNAmarker based analysis.…”

Section: Methodsmentioning

confidence: 99%

Identification of apple genes Md-Exp7 and Md-PG1 alleles in advanced selections resistant to scab

et al. 2022

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The creation of apple varieties with a high level of f lesh f irmness and long shelf life is one of the important goals in breeding. Among the genes controlling these traits, the role of the endogenous ethylene biosynthesis control gene, Md-ACS1, the expansin gene, Md-Exp7, and the polygalacturonase gene, Md-PG1, has been established. The use of DNA marker analysis to solve problems in breeding for fruit quality traits allows one not only to track several target genes simultaneously, but also to cull plants with undesirable alleles at the early stages of development. In order to select complex donors of breeding traits, molecular genetic identif ication of the genes that determine the quality traits of apple fruits Md-Exp7 and Md-PG1 was performed in 256 breeding selections carrying the scab resistance gene Rvi6 and valuable allelic variants of the Md-ACS-1 gene, which determines the endogenous synthesis of ethylene in fruits: 90 samples with the Md-ACS1 allele (2/2) and 166 samples with Md-ACS1 (1/2). As a result of the study, an allelic combination for the Md-Exp7 and Md-PG1 genes was established. Analysis of the parental cultivars (Renet Simirenko, Modi, Smeralda, Renoir, Fulzhion and Granny Smith) used to obtain hybrid selections revealed three alleles 198, 202, 214 bp according to the DNA marker of the Md-Exp7 gene. The SSR marker for the Md-PG1 gene amplif ied three alleles (289, 292, 298 bp) on the abovementioned cultivars. Within the 256 breeding selections samples that have the most priority for breeding alleles of the desired genes in combination with the Rvi6 gene and/or with selection-priority allelic variants of the Md-ACS-1 gene were identif ied. Of the most valuable for breeding, 46 accessions carrying the combination Md-Exp7 (202:202) + Md-ACS1 (2/2) were distinguished. Hybrids with alleles Md-PG1 (292:292) + Md-ACS1 (2/2) are also most valuable for use in breeding and as donors of selection-valuable alleles; 21 samples were identif ied. Accessions with a complex of breeding-valuable target alleles are valuable complex donors, as well as valuable breeding material for creating varieties with improved fruit quality characteristics and scab resistance.

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Section: Methodsmentioning

confidence: 99%

Identification of apple genes Md-Exp7 and Md-PG1 alleles in advanced selections resistant to scab

et al. 2022

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“…Their aesthetic qualities are not lost at any time of the year. Trees of this type are widely used for ornamental purposes [1][2][3][4].…”

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Screening of apple genotypes with the columnar growth habit using control markers

et al. 2020

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One of the approaches to intensify horticulture is to introduce cultivars with an unusual canopy into commercial production. Such plants can be columnar Apple trees. In modern breeding, there is a trend to create cultivars with a compact canopy. In such orchards, it is reduced to a minimum the cost for pruning, harvesting, and protection against pest and disease, which are the main expenses in apple orchards with a traditional canopy. The use of molecular markers linked to columnar growth habit allows us to identify a physiological sign at an early stage of growth: in the juvenile period. The assessment of apple cultivars and hybrids was carried out at the I. V. Michurin Federal Scientific Centre in 2015-2018. Four markers were used in the research: Mdo. chr 10.12, C18470-25831, 29f1, and jwlr to identify plants with the columnar growth habit gene (Co). The use of various DNA markers made it possible to establish that not all of them are well linked to the Co gene. In the research process, primers were identified for markers 29f1 and jwlr, which reliably allowed us to identify plants with columnar growth habit at the juvenile stage, which will significantly reduce the breeding process.

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Genetic resources in Russia: from collections to bioresource centers

Хлесткина

2022

Trudy po prikladnoy botanike, genetike i selektsii

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Collections of bioresources and a set of technologies for their conservation, study and practical use are now the basis of bioeconomy, biosafety, and food security. It is the foundation underpinning production chains, leading from basic research to various technological areas and industries.An analysis of the current state and an assessment of the prospects for the development of bioresource collections in the Russian Federation are presented. Regularities of the transformation from genebanks into bioresource centers and the trend towards integration network interaction among the collections of the same type are considered. The observed trends are analyzed in detail employing the case study of the development of plant genetic resources collections. The current tendencies of their management set by Decrees of the President of the Russian Federation No. 44 “On the National Center for Plant Genetic Resources” and No. 45 “On the Interdepartmental Commission on the Formation, Preservation and Use of Plant Genetic Resources Collections” dated February 8, 2022, are discussed.

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An Integrated Approach to Creating Scab-Resistant Apple: Phytopathological Testing and Marker-Assisted Selection

Cited by 3 publications

References 77 publications

Identification of apple genes <i>Md-Exp7</i> and <i>Md-PG1</i> alleles in advanced selections resistant to scab

Identification of apple genes <i>Md-Exp7</i> and <i>Md-PG1</i> alleles in advanced selections resistant to scab

Screening of apple genotypes with the columnar growth habit using control markers

Genetic resources in Russia: from collections to bioresource centers

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