Cryopreservation of Ribes nigrum (L.) dormant buds: recovery via in vitro culture to the field

Abstract

Cryopreservation of dormant buds can be a feasible method for long term preservation of clonally propagated woody plants.In the present study, dormant buds of blackcurrant (Ribes nigrum L) cultivar Mortti were cryopreserved. Twig segments from greenhouse and field grown plants were cooled at their natural moisture content at the rate of 0.17 °C min −1 from zero to −38 °C, immersed in liquid nitrogen and stored in liquid nitrogen vapor. The post-cryopreservation regrowth of buds was evaluated in vitro and viability of recovered plants was tested in vivo. The estimated recovery rate for buds thawed after 1-6 days in cryostorage was 86% and 66% for outdoor and greenhouse grown buds, respectively. Microplants adequate in size and quality grew successfully in vivo and plants produced berries in field. After 4 years in cryostorage, the estimated recovery rate for outdoor buds was 58%. According to results from 1 to 6 days cryostorage, the tested dormant bud protocol turned to be applicable for cryopreservation of the blackcurrant cultivar Mortti. However, concerning the long-term preservation the decline of recovery is important to consider in the future experiments. Key messageThe cryopreservation of dormant buds combined with in vitro recovery provides a feasible tool for conservation of plant genetic resources of blackcurrant.

Storage of Pineapple Shoot Tips in Liquid Nitrogen for Three Years Does Not Modify Field Performance of Recovered Plants

“…However, it is important to assess the resilience of cryostored plants when grown under eld conditions as this is ultimately the system used for production. Rantala et al (2019) investigated the recovery of cryopreserved shoot tips of blackcurrant and recorded the yield of berries harvested in two consecutive seasons. As reported in the present study, those authors found no differences in the yield of cryostored and non-stored plants.…”

Kriopreservasi Mata Tunas Ubi Kayu Lokal Indonesia: Faktor Penentu Keberhasilan

“…yang sesuai seperti adanya auksin dan senyawa penting lainnya untuk berakar di plantlet Lavandula angustifolia L. Penyimpanan dalam nitrogen cair selama 45 menit atau 1 jam sudah dapat menjadi indikator kemampuan sampel mata tunas disimpan dalam jangka panjang (dorman). Penyimpanan dalam uap nitrogen dengan kisaran suhu sekitar -140˚C juga dilakukan pada tunas dorman dari kebun buah Ribes nigrum L [15]. Hasil ini memberikan harapan bahwa stek super mini dapat disimpan dalam jangka panjang.…”

“…Hal ini dikarenakan kandungan air yang banyak akan menjadi kristal es bila direndam di nitrogen cair. Pada tanaman buah berkayu lainnya, perlakuan slow cooling (pendinginan bertahap) sebelum dicelup di nitrogen cair lalu disimpan hanya dalam uap nitrogen cair juga merupakan yang terbaik seperti pada mata tunas dorman Ribes nigrum L. dengan daya hidup mencapai 83% [15].…”

Droplet vitrification technique for cryopreservation of a large diversity of blackcurrant (Ribes nigrum L.) cultivars

“…Cryopreservation of blackcurrant has been studied for a long time using a variety of cryopreservation methods and protocols (Benson et al 1996;Reed et al 2001Reed et al , 2004Reed et al , 2005Johnston et al 2007;Harding et al 2009;Green and Grout 2010;Rantala et al 2019). Different cryopreservation techniques are employed for Ribes species in USDA-ARS National Plant Germplasm System, Plant and Animal Genetic Resources Preservation Unit, Fort Collins, CO (Jenderek and Reed 2017).…”