Freeze-preservation of buds from Scots pine trees

Kuoksa, T.H.; Hohtola, Anja

doi:10.1007/bf00048212

Cited by 16 publications

(2 citation statements)

References 18 publications

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“…; Matsumoto et al, 2001), silver birch (Betula pendula Roth. ; Ryynänen, 1996), Scots pine (Pinus sylvestris L.; Kuoksa and Hohtola, 1991), willow (Salix sp. ; Towill and Widrlechner, 2004), and several other species (Towill and Ellis, 2008).…”

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confidence: 99%

Effect of Geographical Location, Year, and Cultivar on Survival of Malus sp. Dormant Buds Stored in Vapors of Liquid Nitrogen

Jenderek¹,

Forsline²,

Postman³

et al. 2011

horts

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Clonal woody crop germplasm collections often originate and are grown in distinct geographical locations. Because the degree of cold-hardiness is known to be a factor in the successful use of dormant bud cryopreservation for Malus, it was suggested that material from relatively warmer climates would not respond to cryopreservation as well as material from colder environments. To test this hypothesis, the effect of growing provenance on cryosurvival of dormant buds from three Malus (apple) cultivars grown in three locations (Geneva, NY; Davis, CA; and Corvallis, OR) was tested in 3 consecutive years. Dormant winter buds were harvested at the three locations, cryopreserved, and bud viability was tested by grafting. The collective 3-year mean viability for cryopreserved dormant apple buds for the three locations ranged from 63% to 81% of the buds surviving with the highest survival from the Corvallis site; however, the Geneva twigs were exposed to the lowest preharvest temperature. These results suggest that the temperature at the growing location may not hinder application of the dormant bud cryopreservation method with Malus to the extent previously speculated.

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Effect of Geographical Location, Year, and Cultivar on Survival of Malus sp. Dormant Buds Stored in Vapors of Liquid Nitrogen

Jenderek¹,

Forsline²,

Postman³

et al. 2011

horts

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“…In cryopreservation, the use of dormant buds (DB) costs less, is simpler to accomplish and requires less time than using shoot tips because it does not involve aseptic cultures and the time consuming excision of shoots. The possibility of preserving plant dormant buds in liquid nitrogen has been known for years [17] and was reported in Malus [3,18,21,27], Fraxinus [29], Ulmus [4], Morus [8,10], Pyrus [23], Pinus sylvestris [6], Betula pendula [16], Prunus cerasus [25], and other tree species. Despite the successful reports, the dormant bud method is not widely practiced in plant germplasm preservation.…”

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Twig pre-harvest temperature significantly influences effective cryopreservation of Vaccinium dormant buds

Jenderek

Tanner

Ambruzs³

et al. 2017

Cryobiology

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Cryopreservation of temperate woody-plant material by dormant buds is less expensive than using shoot tips isolated from tissue cultured plants; however currently, dormant buds are used only for preservation of selected temperate tree and shrub species. Using dormant buds could be an efficient strategy for long-term preservation of blueberry (Vaccinium L.) genetic resources. In this study, viability of V. hybrid 'Northsky' (PI 554943) dormant buds was evaluated at 30 harvest dates over three consecutive fall/winter seasons to determine the optimal harvest time that promotes high post cryopreservation viability. Twigs with dormant buds were cut into 70 mm segments containing at least two nodes, desiccated, slowly cooled, stored in liquid nitrogen vapor and tested for post-cryopreservation regrowth. The highest regrowth of cryopreserved dormant buds was observed for buds harvested in mid-December and during the first half of January. Pearson's correlation coefficients were computed to evaluate the association between bud characteristics and viability at harvest date and logistic regression models were fit to test the ability of twig characteristics and temperatures to predict post cryopreservation bud viability. Post-cryopreservation viability was negatively correlated (p < 0.05) with average minimum, maximum and daily mean temperature preceding the bud harvest but was not correlated with the dormant bud initial and end moisture content, twig diameter, the number of dormant buds/cm of twig length and the number of days in desiccation. Regression tree analysis suggested post-cryopreservation viability to be between 52 and 80% for dormant buds harvested after a 10 day average maximum air temperature of <11.2 °C. Pre-harvest air temperature was a significant indicator of optimal dormant bud harvest time to produce adequate viability for long term preservation of blueberry genetic resources.

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Introduction to the in Vitro Preservation of Plant Cells, Tissues and Organs

Grout¹

1995

Genetic Preservation of Plant Cells in Vitro

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Freeze-preservation of buds from Scots pine trees

Cited by 16 publications

References 18 publications

Effect of Geographical Location, Year, and Cultivar on Survival of Malus sp. Dormant Buds Stored in Vapors of Liquid Nitrogen

Effect of Geographical Location, Year, and Cultivar on Survival of Malus sp. Dormant Buds Stored in Vapors of Liquid Nitrogen

Twig pre-harvest temperature significantly influences effective cryopreservation of Vaccinium dormant buds

Introduction to the in Vitro Preservation of Plant Cells, Tissues and Organs

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