Twig pre-harvest temperature significantly influences effective cryopreservation of Vaccinium dormant buds

Abstract

Cryopreservation of temperate woody-plant material by dormant buds is less expensive than using shoot tips isolated from tissue cultured plants; however currently, dormant buds are used only for preservation of selected temperate tree and shrub species. Using dormant buds could be an efficient strategy for long-term preservation of blueberry (Vaccinium L.) genetic resources. In this study, viability of V. hybrid 'Northsky' (PI 554943) dormant buds was evaluated at 30 harvest dates over three consecutive fall/winter seasons to determine the optimal harvest time that promotes high post cryopreservation viability. Twigs with dormant buds were cut into 70 mm segments containing at least two nodes, desiccated, slowly cooled, stored in liquid nitrogen vapor and tested for post-cryopreservation regrowth. The highest regrowth of cryopreserved dormant buds was observed for buds harvested in mid-December and during the first half of January. Pearson's correlation coefficients were computed to evaluate the association between bud characteristics and viability at harvest date and logistic regression models were fit to test the ability of twig characteristics and temperatures to predict post cryopreservation bud viability. Post-cryopreservation viability was negatively correlated (p < 0.05) with average minimum, maximum and daily mean temperature preceding the bud harvest but was not correlated with the dormant bud initial and end moisture content, twig diameter, the number of dormant buds/cm of twig length and the number of days in desiccation. Regression tree analysis suggested post-cryopreservation viability to be between 52 and 80% for dormant buds harvested after a 10 day average maximum air temperature of <11.2 °C. Pre-harvest air temperature was a significant indicator of optimal dormant bud harvest time to produce adequate viability for long term preservation of blueberry genetic resources.

Salix dormant bud cryotolerance varies by taxon, harvest year, and stem‐segment length

“…In each year of the study, the branches were harvested between 14 and 17 January. Studies on cryopreservation of Vaccinium L. DBs showed a strong influence of average air temperature 10 d before the branch harvest on cryopreservation viability (Jenderek et al., 2017); thus, the most favorable time for harvest for cryopreservation may vary from year to year, and weather forecast should be considered in setting twig harvest time for cryopreservation of Salix genetic resources also. It is assumed that in the 2008 season, the dormancy of the buds was already broken when the twigs were harvested, and their viability would have been enhanced with earlier harvest that was not known at that time.…”

Challenges and Prospects for the Conservation of Crop Genetic Resources in Field Genebanks, in In Vitro Collections and/or in Liquid Nitrogen

“…[91] and sour cherry (Prunus cerasus) [92] have been prepared for conservation with this method. An adapted dormant bud cryopreservation protocol where cryopreserved dormant buds are not grafted but directly planted out is developed for Vaccinium [87]. Alternatively, buds can be recovered in vitro as in the case of mulberry (Morus sp.)…”

Kriopreservasi Mata Tunas Ubi Kayu Lokal Indonesia: Faktor Penentu Keberhasilan

“…Pemecahan dormansi dapat dilakukan dengan pemberian stress, namun pada ubi kayu, setelah penyimpanan dalam nitrogen cair baik pada perlakuan larutan krioprotektan kombinasi dengan 5% maupun 10% DMSO, mata tunas yang belum atau tidak merekah mempunyai daya hidup lebih rendah bahkan tidak tumbuh hingga umur 48 HST (Gambar 8 dan 9). Hal ini berbeda dengan jenis tanaman lain seperti kelompok blueberry (Vaccinium) pada penelitian yang dilakukan [16] yang menggunakan mata tunas dorman dari cabang. Gambar 6.…”

“…Pengambilan tunas dorman pada suhu ini sangat penting sebelum diperlakukan penyimpanan di uap nitrogen cair [16]. Hal ini sesuai dengan hasil yaitu stek mini ubi kayu dengan 1-2 mata tunas akan mempunyai daya hidup lebih tinggi bila hanya menggunakan uap nitrogen cair, tidak direndam.…”