Decreased cell survival and DNA repair capacity after UVC irradiation in association with down-regulation of GRP78/BiP in human RSa cells

Xylomexicanins C and D, New Mexicanolide-Type Limonoids from Xylocarpus granatum

“…The cell survival was evaluated by an MTT assay, as described elsewhere. 16) Briefly, logarithmically proliferating cells were plated into 96-well plates (1 Â 10 4 cells/well) with the medium containing compounds 1 and 2 at the indicated doses, and culturing for 2 d. After the culture, the activity of mitochondrial succinic dehydrogenase was measured by further incubating the cells with 0.5 mg/mL of MTT (Sigma) for 4 h, before estimating the absorbance at 570 nm with a reference wavelength of 655 nm. Cell survival was calculated by the absorbance as a percentage of the surviving cells.…”

The Involvement of Annexin II in Resistance to UVB-Induced Cell Death and in the Increased Nucleotide Excision Repair Capacity of UV-Damaged DNA in Human Cells

“…Cell susceptibility to UVB-induced cell death was measured by a colony survival assay, as previously reported. 17) In brief, cells were plated in 100-mm dishes (1 Â 10 3 cells/dish), incubated for 24 h, and then irradiated with UVB at the indicated dosages and cultured for 14 d, followed by colony counting. The D 0 value, the dose at which survival is reduced by 63% at any point on the linear portion of the curve, 10) was calculated.…”

The Roles of HSP27 and Annexin II in Resistance to UVC-Induced Cell Death: Comparative Studies of the Human UVC-Sensitive and -Resistant Cell Lines RSa and APr-1

“…The susceptibility of the cells to UVC-induced cell death was measured by colony survival assay and methylthiazole tetrazolium (MTT) metabolic viability assay, as reported previously. 17) …”