The Roles of HSP27 and Annexin II in Resistance to UVC-Induced Cell Death: Comparative Studies of the Human UVC-Sensitive and -Resistant Cell Lines RSa and AP^r-1

The Involvement of Annexin II in Resistance to UVB-Induced Cell Death and in the Increased Nucleotide Excision Repair Capacity of UV-Damaged DNA in Human Cells

“…Although the basal protein levels of annexin II did not differ between the two cell lines, they decreased in the RSa cells after UVC irradiation, but not in the AP r -1 cells. 18) A similar change in annexin II may also occur after UVB irradiation and might be related to the difference in susceptibility to UVB between the two cell lines.…”

“…Immunoblotting analysis was carried out as previously described. 10,11,18) In brief, cells were washed once with phosphate-buffered saline (PBS), and whole cells were lysed with SDS-sample buffer and boiled for 5 min. Whole-cell lysates were separated by 12% SDS gel and transferred onto a PVDF membrane (Millipore, Billerica, MA).…”

“…RSa cells grown to 60-80% confluence in 100-mm dishes were transfected with the annexin II expression vector, ANX II/pcDNA 3.1(þ), 18) and pcDNA 3.1(þ) using LipofectamineÔ LTX Reagent (Invitrogen) according to the manufacturer's instructions. To analyze the susceptibility to UVB-induced cell death of the cDNA-transfected cells, 24 h after transfection the cells were replated (1 Â 10 3 cells/100-mm dish), cultured for 24 h, and then irradiated with UVB, followed by the colony survival assay.…”

UVC Mutagenicity Is Suppressed in Japanese Miso-Treated Human RSa Cells, PossiblyviaGRP78 Expression

“…Recently we suggested that HSP27 might play a role in DNA repair, regulating the metabolism of nucleotide excision repair enzymes in the nucleus. 21,36,37) The molecular mechanisms underlying the suppression of UVC mutagenicity by miso samples remain unknown, but we speculate that changes in the expression of chaperones such as HSP27 and GRP78 are implicated in these mechanisms. Forty-eight h after GRP78 siRNA or NC siRNA transfection, mRNA levels were analyzed by RT-PCR (A).…”

Prevention of Pancreatic Cancer

“…The activity in variant cells was found to be associated with heat shock protein (HSP) 27 expression, resulting in enhancement of error-free DNA repair function (Wano et al, 2004). A HSP27-bound protein, annexin II, was also suggested to play some roles on the error-free function in human cell nuclei (Tong et al, 2008 andJin et al, 2009). We recently developed a method to analyze base substitution mutation of the K-ras codon 12 and found that a decreased mutation frequency accompanied increased GRP78 expression in human RSa cells (Hirano et al, 2008 andZhai et al, 2005).…”