UVC Mutagenicity Is Suppressed in Japanese Miso-Treated Human RSa Cells, Possibly<i>via</i>GRP78 Expression

Jiang, Xia; Ren, Qian; Chen, Shiping; Tong, Xiao-Bo; Dong, Ming; Sugaya, Shigeru; Tanaka, Takeshi; Kita, K; Suzuki, Nobuo

doi:10.1271/bbb.110175

Cited by 7 publications

(8 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, it is possible that concentration of miso ingredients in the liver after absorption might be higher than those concentration. There are three types of miso: rice-koji miso, soybean-koji miso and barley-koji miso 11 . In the present study, we used rice-koji miso.…”

Section: Discussionmentioning

confidence: 99%

“…Two types of Japanese rice-koji miso, Kurasaigetsuusujiomiso (KU) and Igoumiso (IG), were purchased from Ando Brewery (Kakunodate, Japan) 11 . KU is made from rice (Kitauramura, Akita, Japan), soy (Akita, Japan), and salt with special Yurara yeast (Akita Japan) 16 .…”

Section: Methodsmentioning

confidence: 99%

“…IG is made from rice (Kitauramura, Akita, Japan), soy with performing husk-removing treatment (Japan), and salt 16 . Miso extracts were prepared as previously described 11 , and the supernatant was then filtered through a 0.45 µm membrane (IWAKI Glass, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…Miso is a traditional Japanese seasoning and is used for miso soup, a Japanese culinary staple. It has also been reported that Japanese miso extract increases GRP78 expression and suppresses ultraviolet C mutagenesis 11 . In the present study, we examined whether Japanese miso extracts could suppress HAV replication in human hepatocytes.…”

Section: Introductionmentioning

confidence: 95%

See 3 more Smart Citations

Inhibitory effect of Japanese rice-koji miso extracts on hepatitis A virus replication in association with the elevation of glucose-regulated protein 78 expression

et al. 2018

Self Cite

View full text Add to dashboard Cite

Hepatitis A virus (HAV) infection is one of the major causes of acute hepatitis and acute liver failure in developing and developed countries. Although effective vaccines for HAV infection are available, outbreaks of HAV infection still cause deaths, even in developed countries. One approach to control HAV infection is prevention through diet, which can inhibit HAV propagation and replication. Glucose-regulated protein 78 (GRP78) is a member of the heat shock protein 70 family of molecular chaperone required for endoplasmic reticulum stress and stress-induced autophagy. We previously showed that the elevation of GRP78 expression inhibits HAV replication. It has been reported that Japanese miso extracts, which was made from rice-koji, enhance GRP78 expression. In the present study, we used human hepatoma Huh7 cells and human hepatocyte PXB cells to examine the efficacy of Japanese miso extracts as antiviral agents against HAV. Japanese miso extracts enhanced GRP78 expression and inhibited HAV replication in human hepatocytes. Together, these results demonstrate that Japanese miso extracts may partly modulate GRP78 expression and additively or synergistically work as antivirals against HAV infection. Japanese miso extracts can be used as effective dietary supplements for severe hepatitis A.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

See 2 more Smart Citations

Inhibitory effect of Japanese rice-koji miso extracts on hepatitis A virus replication in association with the elevation of glucose-regulated protein 78 expression

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Duplex small interfering RNA (siRNA) with Stealth modification against human GRP78 ( GRP78 siRNA) was synthesized based on the nucleotide sequence (Invitrogen), as previously described (11). Stealth RNAi negative control duplex (NC siRNA), with a GC content similar to that of the above Stealth RNAi, was used as a negative control.…”

Section: Methodsmentioning

confidence: 99%

Involvement of heat shock protein 27 in the susceptibility of KT human breast cancer cells to UVC and interferon lethality

Tong

Kita

Chen

et al. 2012

Experimental and Therapeutic Medicine

Self Cite

View full text Add to dashboard Cite

Revealing the key molecules regulating the stress-response pathways in human cells is an intriguing problem. Chaperones, such as glucose-regulated protein 78 (GRP78) and heat shock protein 27 (HSP27), are important molecules for protecting the viability of human cells; however, it remains to be further clarified whether the molecules differentially modulate cellular responses to various types of stressors, such as DNA-damaging ultraviolet ray C (principally 254-nm wavelength, UVC) and cytocidal cytokine interferons. In the present study, the human breast cancer cell lines KT and MCF-7 were examined for GRP78 and HSP27 expression following exposure to UVC and human interferon-β (HuIFN-β). The KT cells demonstrated a higher sensitivity to both UVC and HuIFN-β lethality than MCF-7 cells. The cellular expression levels of GRP78 in KT cells, assessed by western blot analysis, were approximately 2-fold higher than that in MCF-7 cells, while the expression of HSP27 in the KT cells was 20% of the expression in the MCF-7 cells. Decreased resistance to UVC lethality was observed in GRP78 siRNA-transfected KT cells. In addition, HSP27 cDNA transfection of KT cells resulted in an increased resistance to UVC lethality. The cDNA-transfected KT cells showed an increased viability against HuIFN-β, compared with that of empty vector-transfected cells. By contrast, KT cells pretreated with HuIFN-β and irradiated with UVC demonstrated an increased resistance to UVC lethality, in association with increased levels of HSP27 expression. Thus, HSP27 may control the survival response pathways to both UVC and HuIFN-β in the human cells examined.

show abstract

Antiviral Activity of Zinc Sulfate Against Hepatitis a Virus Replication

et al. 2019

View full text Add to dashboard Cite

Aim: Zinc and metallothioneins play roles in antiviral activities during viral infection. We previously reported that Japanese rice-koji miso has an antiviral activity against hepatitis A virus (HAV) infection in hepatocytes. Materials, methods & results: We observed the zinc homeostasis pathway as a key pathway of antiviral activity of Japanese rice-koji miso against HAV infection in hepatocytes using transcriptome-sequencing (RNA-Seq) analysis. Furthermore, zinc sulfate inhibited HAV replicon replication and enhanced glucose-regulated protein 78 expression in a dose-dependent manner. Metallothioneins in conditioned medium were enhanced by zinc sulfate in a dose-independent manner. We also found antiviral activity of zinc sulfate against the replication of the HAV genotype IIIA strain HA11-1299. Conclusion: Zinc may prevent severe hepatitis A.

show abstract

UVC Mutagenicity Is Suppressed in Japanese Miso-Treated Human RSa Cells, PossiblyviaGRP78 Expression

Cited by 7 publications

References 29 publications

Inhibitory effect of Japanese rice-koji miso extracts on hepatitis A virus replication in association with the elevation of glucose-regulated protein 78 expression

Inhibitory effect of Japanese rice-koji miso extracts on hepatitis A virus replication in association with the elevation of glucose-regulated protein 78 expression

Involvement of heat shock protein 27 in the susceptibility of KT human breast cancer cells to UVC and interferon lethality

Antiviral Activity of Zinc Sulfate Against Hepatitis a Virus Replication

Contact Info

Product

Resources

About