Deciphering the Urinary Microbiota Repertoire by Culturomics Reveals Mostly Anaerobic Bacteria From the Gut

Dubourg, Grégory; Morand, Aurélie; Mekhalif, F.; Godefroy, Raphaël; Corthier, Alice; Yacouba, Abdourahamane; Diakite, Ami; Cornu, F.; Cresci, M.; Brahimi, S.; Caputo, Aurélia; Lechevallier, É.; Tsimaratos, Michel; Moal, Valérie; Lagier, Jean-Christophe; Raoult, Didier

doi:10.3389/fmicb.2020.513305

Cited by 45 publications

(29 citation statements)

References 46 publications

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“…However, a recent study proposes that the origin of the urinary microbiota is the gut. These authors show that 64% of species identified in urine samples, using culture and 16S rRNA gene sequencing-based methods, overlap with identified species in the gut microbiota, while only 31% overlap with species isolated from the vagina ( Dubourg et al., 2020 ) ( Figure 3B ). Additionally, the reduction of UTI incidence after fecal microbiota transplantation seems to support this hypothesis ( Tariq et al., 2017 ).…”

Section: Urinary Microbiome or Urobiomementioning

confidence: 99%

Urinary Microbiome: Yin and Yang of the Urinary Tract

Pérez-Carrasco

Soriano‐Lerma

Soriano

et al. 2021

Front. Cell. Infect. Microbiol.

109

119

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The application of next generation sequencing techniques has allowed the characterization of the urinary tract microbiome and has led to the rejection of the pre-established concept of sterility in the urinary bladder. Not only have microbial communities in the urinary tract been implicated in the maintenance of health but alterations in their composition have also been associated with different urinary pathologies, such as urinary tract infections (UTI). Therefore, the study of the urinary microbiome in healthy individuals, as well as its involvement in disease through the proliferation of opportunistic pathogens, could open a potential field of study, leading to new insights into prevention, diagnosis and treatment strategies for urinary pathologies. In this review we present an overview of the current state of knowledge about the urinary microbiome in health and disease, as well as its involvement in the development of new therapeutic strategies.

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Section: Urinary Microbiome or Urobiomementioning

confidence: 99%

Urinary Microbiome: Yin and Yang of the Urinary Tract

Pérez-Carrasco

Soriano‐Lerma

Soriano

et al. 2021

Front. Cell. Infect. Microbiol.

109

119

View full text Add to dashboard Cite

show abstract

“…Moreover, in a recent approach to decipher the urinary microbiota repertoire with the use of culturomics, a research group from France tested 435 urine samples and isolated a total of 450 diverse bacterial species—including 256 previously not found in urine and 18 completely new species [ 73 ]. This study not only increased the known urinary microbiome repertoire by 39%, but it has also shown that 64.1% of bacterial species were previously isolated from gut microbiota, compared to only 31.7% previously found in the vagina.…”

Section: The Increasingly Appreciated Significance Of the Urinary mentioning

confidence: 99%

“…This study not only increased the known urinary microbiome repertoire by 39%, but it has also shown that 64.1% of bacterial species were previously isolated from gut microbiota, compared to only 31.7% previously found in the vagina. As a result, this study calls for a paradigm shift to look at the urinary microbiota as mostly derived from the intestinal tract [ 73 ], but more research is needed to confirm and ascertain this point-of-origin hypothesis.…”

Section: The Increasingly Appreciated Significance Of the Urinary mentioning

confidence: 99%

The Role of Gut, Vaginal, and Urinary Microbiome in Urinary Tract Infections: From Bench to Bedside

et al. 2020

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The current paradigm of urinary tract infection (UTI) pathogenesis takes into account the contamination of the periurethral space by specific uropathogens residing in the gut, which is followed by urethral colonization and pathogen ascension to the urinary bladder. Consequently, studying the relationship between gut microbiota and the subsequent development of bacteriuria and UTI represents an important field of research. However, the well-established diagnostic and therapeutic paradigm for urinary tract infections (UTIs) has come into question with the discovery of a multifaceted, symbiotic microbiome in the healthy urogenital tract. More specifically, emerging data suggest that vaginal dysbiosis may result in Escherichia coli colonization and prompt recurrent UTIs, while urinary microbiome perturbations may precede the development of UTIs and other pathologic conditions of the urinary system. The question is whether these findings can be exploited for risk reduction and treatment purposes. This review aimed to appraise the three aforementioned specific microbiomes regarding their potential influence on UTI development by focusing on the recent studies in the field and assessing the potential linkages between these different niches, as well as evaluating the state of translational research for novel therapeutic and preventative approaches.

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“…A more recent study employing culturomics, particularly those targeting anaerobes, identified that 64.1% of the bacterial species recovered at least once from urine microbiota had previously been isolated from gut microbiota, whereas only 31.7% had previously been isolated from vaginal microbiota. These findings imply that many aerobic members of the microbiota in the urinary system come from the gut [35]. Therefore, advances in urine analysis technology would allow for the accurate characterization of urinary tract bacteria communities.…”

Section: Urinary Microbiome Of Healthy Womenmentioning

confidence: 99%

The Microbiome’s Function in Disorders of the Urinary Bladder

Choi

Lee

Kim

2021

Applied Microbiology

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The introduction of next generation sequencing techniques has enabled the characterization of the urinary tract microbiome, which resulted in the rejection of the long-held notion of urinary bladder sterility. Since the discovery and confirmation of the human bladder microbiome, an increasing number of studies have defined this microbial community and understand better its relationship to urinary pathologies. The composition of microbial communities in the urinary tract is linked to a variety of urinary diseases. The purpose of this review is to provide an overview of current information about the urinary microbiome and diseases as well as the development of novel treatment methods.

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Deciphering the Urinary Microbiota Repertoire by Culturomics Reveals Mostly Anaerobic Bacteria From the Gut

Cited by 45 publications

References 46 publications

Urinary Microbiome: Yin and Yang of the Urinary Tract

Urinary Microbiome: Yin and Yang of the Urinary Tract

The Role of Gut, Vaginal, and Urinary Microbiome in Urinary Tract Infections: From Bench to Bedside

The Microbiome’s Function in Disorders of the Urinary Bladder

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