Decidual expression and regulation of Fatty acid desaturase 3 during mouse decidualization

Lin, Shuai; Zhu, Yuyuan; Hu, Wei; Yang, Yan; Luo, Jiamei; Hu, Shijun; Yang, Zeng-Ming

doi:10.1530/rep-18-0145

Cited by 4 publications

(3 citation statements)

References 33 publications

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“…Western blot was performed as previously described [ 45 , 46 ]. Briefly, the tissues were homogenized in ice-cold homogenization lysis buffer (150 mM NaCl; 50 mM Tris-HCl, pH 7.5; 1% Triton X-100; and 0.25% sodium deoxycholate), which contained the Protease Inhibitor Cocktail (Roche, Penzberg, Germany).…”

Section: Methodsmentioning

confidence: 99%

Effects of transport stress on pathological injury and expression of main heat shock proteins in the caprine stomach

Tian

Yang

et al. 2020

BMC Vet Res

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Background Transportation is necessary to introduce new breeds of goats to the farm and move the adult meat goat from the farm to the slaughterhouse. However, these actions may give rise to transport stress. Heat shock proteins (HSPs) are playing some important regulate roles during transport stress. The aim of this study was to evaluate the effects of transport stress on the pathological injury and HSPs expression in the stomach of goats. A total of three batches of Ganxi goats from western Jiangxi province were enrolled in this study. For each batch, twelve healthy adult male goats were randomly divided into three groups (four goats per batch and per group): Control group, stress group transported during 2 h and stress group transported during 6 h. Results Our results showed that the different degrees of stomach walls damage, with the change of expression levels of heat shock protein 27 (HSP27), heat shock protein 70 (HSP70) and heat shock protein 90 (HSP90), occurred after goats transportation. In rumen, the mRNA and protein expressions of HSP27 and HSP70 were increased after transport stress, but not HSP90. In reticulum, all three HSPs mRNA and protein levels were upregulated after 2 h transport, but decreased after 6 h transport. In omasum, HSP27 and HSP70 mRNA and protein were increased after transport stress, however, HSP90 mRNA level only had a slightly enhancement after transport stress. In abomasum, HSP70 and HSP90 mRNA and protein levels were increased after transport stress, but HSP27 was decreased after transport stress. Conclusions Taken together, these results revealed that the pathological changes in the gastric tissues and the stomach HSPs expression in goats are related to transport stress and duration. Moreover, this study also provides some new data to advocate reducing transport stress of goats and improving animal welfare.

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Section: Methodsmentioning

confidence: 99%

Effects of transport stress on pathological injury and expression of main heat shock proteins in the caprine stomach

Tian

Yang

et al. 2020

BMC Vet Res

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“…Western blot was performed as previously described [ 37 , 38 ]. Briefly, 50 mg of the small intestine samples were frozen at -80 °C, and 1 mL of lysate was added and homogenized on ice with a homogenizer.…”

Section: Methodsmentioning

confidence: 99%

Effects of transport stress on the oxidative index, apoptosis and autophagy in the small intestine of caprine

Peng

Gao

et al. 2023

BMC Vet Res

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Background Introducing new goat breeds or transferring adult goats from farms to slaughterhouses requires transportation, which can engender adverse effects, such as oxidative stress, pathological cell apoptosis and autophagy. Current evidence suggests that malondialdehyde (MDA) is a metabolite of lipid peroxidation during oxidative stress, while superoxide dismutase (SOD) and catalase (CAT) can alleviate injury caused by free radicals and reactive oxygen species (ROS). Meanwhile, Bcl-2, Bax, LC3B, PINK1 and Parkin are important proteins that participate in pathological cell apoptosis and autophagy. This study aimed to investigate the effects of transportation stress on oxidative stress indexes and expressions of Bcl-2, Bax, LC3B, PINK1 and Parkin in the small intestine of goats. Twelve healthy adult male goats from western Jiangxi province were randomly divided into control, 2 h transportation stress, and 6 h transportation stress groups (n = 4 per group). Results Our results showed that MDA in the small intestine significantly increased after transportation, while SOD and CAT activities decreased, with a significantly increased apoptosis rate of the small intestine cells. The jejunum and duodenum exhibited the highest apoptosis rate in the 2 h and 6 h transportation groups, respectively. The expression of apoptosis-related genes Bcl-2 and Bax and their corresponding proteins exhibited varying degrees of down-regulation or up-regulation, while Bcl-2 and Bax genes in the small intestine were upregulated in the 6 h transportation group. In addition, autophagosomes and autophagolysosomes were found in various parts of the small intestine by transmission electron microscopy, and autophagy-related genes LC3B, PINK1 and Parkin were significantly down-regulated in the 2 h group and up-regulated in the 6 h group. Conclusions Our results indicate that the contents of MDA, SOD and CAT in the small intestine, the expression of pathologic apoptosis-related genes Bcl-2 and Bax, and autophagy-related genes LC3B, PINK1 and Parkin correlated with stress duration caused by transportation. Moreover, this study provides a foothold for further studies on the mechanism of transportation stress in goats and improving animal welfare.

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“…In situ hybridization was performed as previously described [14]. In brief, uteri were cut into 10 mm frozen sections on 3-aminopropyltriethoxy silane (Sigma)-treated slides and fixed in 4% paraformaldehyde solution in PBS.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Oncostatin M expression in the mouse uterus during early pregnancy promotes embryo implantation and decidualization

Zheng

Zhang

et al. 2019

FEBS Letters

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Oncostatin M (OSM) is a member of the interleukin‐6 (IL‐6) family, which functions in embryo implantation and decidualization. The expression, function and regulation of Osm in mouse uteri during early pregnancy remain unknown. We show that Osm is mainly expressed in the uterine epithelium from days 1 to 4 of pregnancy and in decidual cells on day 5 of pregnancy. Osm promotes the attachment of Osm‐soaked blue beads, which mimic blastocysts, to a pseudopregnant mouse uterus. Prostaglandin E2 (PGE2)‐induced Osm in mouse uterine epithelial cells upregulates the levels of Il‐33 expression and phosphorylates Stat3. In vitro decidualization is significantly promoted by Osm. Our results indicate that PGE2‐induced Osm may mediate embryo implantation through Il‐33 and participate in decidualization via the Stat3‐Egr1 pathway.

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Decidual expression and regulation of Fatty acid desaturase 3 during mouse decidualization

Cited by 4 publications

References 33 publications

Effects of transport stress on pathological injury and expression of main heat shock proteins in the caprine stomach

Effects of transport stress on pathological injury and expression of main heat shock proteins in the caprine stomach

Effects of transport stress on the oxidative index, apoptosis and autophagy in the small intestine of caprine

Oncostatin M expression in the mouse uterus during early pregnancy promotes embryo implantation and decidualization

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