Oncostatin M expression in the mouse uterus during early pregnancy promotes embryo implantation and decidualization

Fu, Tao; Zheng, Hong-Tao; Zhang, Haiyi; Chen, Zi-Cong; Li, Bo; Yang, Zeng‐Ming

doi:10.1002/1873-3468.13468

Cited by 15 publications

(13 citation statements)

References 38 publications

(76 reference statements)

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“…Immunohistochemistry was performed as described previously [39]. In short, paraffin sections (5 μm) were deparaffined, rehydrated, and antigen retrieved by boiling in 10 mM citrate buffer for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Fibroblast activation during decidualization: Embryo-derived TNFα induction of PGI2-PPARδ-ACTIVIN A pathway through luminal epithelium

Chen

Shi

Lin

et al. 2022

Preprint

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Objectives: Human endometrium undergoes cyclical shedding and bleeding, scar-free repair and regeneration in subsequent cycles. Fibroblast activation has been shown to play a key role during normal tissue repair and scar formation. Abnormal fibroblast activation leads to fibrosis. Fibrosis is the main cause of intrauterine adhesion, uterine scaring, and thin endometrium. Endometrial decidualization is a critical step during early pregnancy. There are 75% of pregnancy failures pointed to decidualization defects. Because fibroblast activation and decidualization share similar markers, we assumed that fibroblast activation should be involved in decidualization. Materials and Methods: Both pregnant and pseudopregnant ICR mice were used in this study. Immunofluorescence and immunohistochemistry were applied to examine fibroblast activation-related markers in mouse uteri. Western blotting was used to identify the impact on decidualization. Western blot and RT were used to show how arachidonic acid and its downstream product prostaglandin activate fibroblasts. Additionally, embryo-derived TNFα was shown to stimulate the secretion of arachidonic acid by immunofluorescence, western blot, and ELASA. The aborted decidual tissues with fetal trisomy 16 were compared with control tissues. GraphPad Prism5.0 Student's t test was used to compare differences between control and treatment groups. Results: Fibroblast activation-related markers are obviously detected in pregnant decidua and under in vitro decidualization. ACTIVIN A secreted under fibroblast activation promotes in vitro decidualization. We showed that arachidonic acid released from uterine luminal epithelium can induce fibroblast activation and decidualization through PGI2 and its nuclear receptor PPAR-δ. Based on the significant difference of fibroblast activation-related markers between pregnant and pseudopregnant mice, we found that embryo-derived TNFα promotes cPLA2α phosphorylation and arachidonic acid release from luminal epithelium. Fibroblast activation is also detected under human in vitro decidualization. Similar arachidonic acid-PGI2-PPARδ-ACTIVIN A pathway 51 is conserved in human endometrium. Compared to controls, fibroblast activation is obviously compromised in human decidual tissues with fetal trisomy 16. Conclusions: Embryo-derived TNFα promotes cPLA2α phosphorylation and arachidonic acid release from luminal epithelium to induce fibroblast activation and decidualization.

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Section: Methodsmentioning

confidence: 99%

Fibroblast activation during decidualization: Embryo-derived TNFα induction of PGI2-PPARδ-ACTIVIN A pathway through luminal epithelium

Chen

Shi

Lin

et al. 2022

Preprint

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“…Immunohistochemistry was performed as previously described [ 43 , 44 ]. The paraffin sections (5 μm) were deparaffinized in xylene, rehydrated with a graded series of ethanol, and washed in water.…”

Section: Methodsmentioning

confidence: 99%

Effects of transport stress on pathological injury and expression of main heat shock proteins in the caprine stomach

Tian

Yang

et al. 2020

BMC Vet Res

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Background Transportation is necessary to introduce new breeds of goats to the farm and move the adult meat goat from the farm to the slaughterhouse. However, these actions may give rise to transport stress. Heat shock proteins (HSPs) are playing some important regulate roles during transport stress. The aim of this study was to evaluate the effects of transport stress on the pathological injury and HSPs expression in the stomach of goats. A total of three batches of Ganxi goats from western Jiangxi province were enrolled in this study. For each batch, twelve healthy adult male goats were randomly divided into three groups (four goats per batch and per group): Control group, stress group transported during 2 h and stress group transported during 6 h. Results Our results showed that the different degrees of stomach walls damage, with the change of expression levels of heat shock protein 27 (HSP27), heat shock protein 70 (HSP70) and heat shock protein 90 (HSP90), occurred after goats transportation. In rumen, the mRNA and protein expressions of HSP27 and HSP70 were increased after transport stress, but not HSP90. In reticulum, all three HSPs mRNA and protein levels were upregulated after 2 h transport, but decreased after 6 h transport. In omasum, HSP27 and HSP70 mRNA and protein were increased after transport stress, however, HSP90 mRNA level only had a slightly enhancement after transport stress. In abomasum, HSP70 and HSP90 mRNA and protein levels were increased after transport stress, but HSP27 was decreased after transport stress. Conclusions Taken together, these results revealed that the pathological changes in the gastric tissues and the stomach HSPs expression in goats are related to transport stress and duration. Moreover, this study also provides some new data to advocate reducing transport stress of goats and improving animal welfare.

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“…We also found that sperm cells with abnormal DFI caused a significant increase in OSM expression in the epithelial cells compared with the normal DFI group. OSM, a cytokine from the IL-6 family, is an activator of the JAK/STAT signalling pathway that has an essential role in fertilization, embryo implantation, uterine decidualization and early pregnancy (Fu et al, 2019;Wahl & Wallace, 2001), and its expression by oviduct and endometrial cells was found previously during the interaction of these cell with sperm (Ajdary et al, 2021;Wahl & Wallace, 2001). Activation of this signalling pathway in response to OSM, which is widely expressed throughout the female reproductive tract, was confirmed in cultured human granulosa cells (Pastuschek et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Sperm–oviduct interaction: Differential gene expression of growth factors induced by sperm DNA fragmentation

et al. 2022

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Oncostatin M expression in the mouse uterus during early pregnancy promotes embryo implantation and decidualization

Cited by 15 publications

References 38 publications

Fibroblast activation during decidualization: Embryo-derived TNFα induction of PGI2-PPARδ-ACTIVIN A pathway through luminal epithelium

Fibroblast activation during decidualization: Embryo-derived TNFα induction of PGI2-PPARδ-ACTIVIN A pathway through luminal epithelium

Effects of transport stress on pathological injury and expression of main heat shock proteins in the caprine stomach

Sperm–oviduct interaction: Differential gene expression of growth factors induced by sperm DNA fragmentation

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