De Novo Transcriptome Sequencing in Passiflora edulis Sims to Identify Genes and Signaling Pathways Involved in Cold Tolerance

Liu, Sian; Li, Anding; Chen, Caihui; Cai, Guo-Jun; Zhang, Limin; Guo, Changhong; Xu, Meng

doi:10.3390/f8110435

Cited by 24 publications

(31 citation statements)

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“…De novo transcriptome assembly has been widely applied to detect and identify differential genes under different experimental conditions (He et al, 2017;Liu et al, , 2018Chen et al, 2018), enabling researchers to understand the molecular mechanism of action from a transcriptomics perspective. Different expression gene (DEGs) profiling in this technique presents the advantages of precision, economy, and repeatability, and has been widely used in plants to explore genes related to heat resistance (Li et al, 2015;Yan et al, 2016;Shi et al, 2017), while in insects, comparative transcriptome analysis related to heat responses has been only applied in several species, including G. pyloalis, Cryptolaemus montrouzieri, and Bombyx mori (Wang et al, 2014;Zhang Y.H.…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis of the Heat Stress Response in Monochamus alternatus Hope (Coleoptera: Cerambycidae)

Zhao

Qiao

et al. 2020

Front. Physiol.

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Temperature is a critical factor of insect population abundance and distribution. Monochamus alternatus Hope (Coleoptera: Cerambycidae) is a significant concern since it is transmitted vector of the pinewood nematode posing enormous economic and environmental losses. This pest shows tolerance to heat stress, especially extremely high temperatures. Exposing for 6, 12, 24, 48, or 96 h, the 50% median lethal temperatures (Ltem 50) for fourth-instar larvae were 47.5, 45.5, 43.9, 43.4, and 42.3 • C, respectively. A total of 63,360 unigenes were obtained from complementary DNA libraries of M. alternatus fourth-instar larvae (kept at 25 • C and exposed to 40 • C for 3 h) and annotated with six databases. Five hundred sixty-one genes were significantly upregulated, and 245 genes were downregulated after heat stress. The Gene Ontology enrichment analysis showed that most different expression genes are categorized into "protein folding" and "unfold protein binding" terms. In addition, "Longevity regulating pathway-multiple species," "Antigen processing and presentation" as well as "MAPK signaling pathway" were significantly enriched Kyoto Encyclopedia of Genes and Genomes pathways. Further analysis of different expression genes showed that metabolism processes were suppressed, while ubiquitin proteolytic system, heat shock proteins, immune response, superoxide dismutase, cytochrome P450s, and aldehyde dehydrogenase were induced after heat shock. The stress signaling transduction pathways such as MAPK, Hippo, and JAK-STAT might be central convergence points in M. alternatus heat tolerance mechanism. The expression levels from quantitative realtime PCR of 13 randomly selected genes were consistent with the transcriptome results. These results showed that M. alternatus possessed strong heat tolerance and genes related to protein activity, immune response, and signal transduction composed of a complicated heat tolerance mechanism of M. alternatus. This research provided new insights into the mechanisms of thermal tolerance in other insects and aided in exploring the function of heat resistance-related genes.

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Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis of the Heat Stress Response in Monochamus alternatus Hope (Coleoptera: Cerambycidae)

Zhao

Qiao

et al. 2020

Front. Physiol.

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“…In addition, functional genes of P. edulis have not been well studied, especially by relative quantitative analysis of functional genes, and research on reference genes in P. edulis has not been reported. [1,2]. To enhance the reliability of functional gene expression research, we systematically selected reliable internal control genes for RT-qPCR data normalization in multiple tissue samples and cold-treated samples of P. edulis.…”

Section: Discussionmentioning

confidence: 99%

“…Passi ora edulis (P. edulis), or passion fruit, is a perennial vine that belongs to the Passi oraceae family. P. edulis, which origined in South America, is now widely cultivated in southern China because of its high edible value, medicinal e cacy and ornamental properties; additionally, P. edulis is an important raw material in the juice and pulp industry [1][2][3][4][5][6]. Passion fruit, a tropical fruit, provides a variety of nutrients, such as carbohydrates, vitamins and minerals, which are essential for biological activities [5].…”

Section: Introductionmentioning

confidence: 99%

“…Passion fruit, a tropical fruit, provides a variety of nutrients, such as carbohydrates, vitamins and minerals, which are essential for biological activities [5]. P. edulis was introduced in China at the beginning of the last century [2]. In recent years, researchers have studied the adaptability of P. edulis to cold environments, providing a theoretical basis for expanding the cultivation area of P. edulis in China [1,2].…”

Section: Introductionmentioning

confidence: 99%

“…P. edulis was introduced in China at the beginning of the last century [2]. In recent years, researchers have studied the adaptability of P. edulis to cold environments, providing a theoretical basis for expanding the cultivation area of P. edulis in China [1,2]. Although the edible value and medicinal e cacy of P. edulis have increased, only a few studies on the functional genes of P. edulis have been reported, especially on gene expression patterns under cold conditions.…”

Section: Introductionmentioning

confidence: 99%

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Reference gene selection in multiple Passiflora edulis tissues and under cold stress for RT- qPCR analysis

Tu¹,

Fan²,

Li³

et al. 2020

Preprint

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Background: Passiflora edulis is a tropical fruit with high edible and medicinal values that is widely cultivated in southern China. The development of new P. edulis variety with cold resistance and research on the mechanism of cold resistance are the basis for further promoting the cultivation of this species. In a previous study, our laboratory discovered a cold-resistant variety Pingtang 1, which is used as a material for the study of cold resistance mechanism in P. edulis. However, functional genes of P. edulis have not been well studied, especially via relative quantitative analysis. In addition, research on reference genes in P. edulis has not been reported.Results: We used three tools to test the expression stability of 10 candidate reference genes in multiple tissue samples and cold-treated samples of P. edulis. We found that Ts and EF1 showed the highest expression stability in all samples. Further analysis showed that HIS and EF1 were stably expressed in tissue samples, whereas UBQ and EF1 were stably expressed in cold-treated samples. Interestingly, EF1 was stably expressed in not only multiple tissue samples but also cold-treated samples. To validate the selected reference genes, we used the target gene ICE1 and investigated its expression level in cold-treated leaves. Interestingly, the expression of ICE1 increased with increasing cold treatment times.Conclusions: In this study, we successfully selected reference genes from among 10 candidates for P. edulis RT-qPCR data normalization. We selected Ts and EF1 as reference genes for normalization in all samples. HIS and EF1 were ideal for data normalization in tissue samples, whereas UBQ and EF1 were ideal for cold-treated samples. Our work substantially benefits the study of functional genes in P. edulis.

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HCN‐induced embryo arrest: passion fruit as an ecological trap for fruit flies

Yang

Zheng

Liao

et al. 2023

Pest Management Science

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BACKGROUND: Fruit flies are important economic pests of fruits, vegetables, and nuts all over the world. In this study, a permanent ecological trap, which was created by the ovicidal effect of phytogenic hydrogen cyanide (HCN) liberated from passion fruits due to oviposition by fruit flies and can be used in the pest management, were determined. RESULTS: Observation of fruit fly eggs in Passiflora within the passion fruit cultivation region in southern China, from Aug 2019 to Oct 2020 showed that the exotic Passiflora attracted the native fruit flies to oviposit, but the eggs could not hatch. Using classical staging to categorize embryonic development and fumigation assays, we show that oviposition by fruit fly on passion fruits, release HCN from the cyanogenic mesocarp. Exposure of the eggs to HCN causes arrest of embryonic development and finally the death of eggs. CONCLUSION: Our results reveal that the life cycle of fruit fly in Passiflora is interrupted at the egg stage. Consequently, we predict that this ecological trap may be permanent. Extensive cultivation of the Passiflora vine as a dead-end trap crop may be an effective avenue to reduce populations of fruit fly pests.

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De Novo Transcriptome Sequencing in Passiflora edulis Sims to Identify Genes and Signaling Pathways Involved in Cold Tolerance

Cited by 24 publications

References 50 publications

Comparative Transcriptome Analysis of the Heat Stress Response in Monochamus alternatus Hope (Coleoptera: Cerambycidae)

Comparative Transcriptome Analysis of the Heat Stress Response in Monochamus alternatus Hope (Coleoptera: Cerambycidae)

Reference gene selection in multiple Passiflora edulis tissues and under cold stress for RT- qPCR analysis

HCN‐induced embryo arrest: passion fruit as an ecological trap for fruit flies

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