Cytotoxicity of Newly Developed Ortho MTA Root-end Filling Materials

Lee, Bin-Na; Son, Hye-Ju; Noh, Han-Jin; Koh, Jeong‐Tae; Chang, Hoon-Sang; Hwang, In-Nam; Hwang, Yun-Chan; Oh, Won-Mann

doi:10.1016/j.joen.2012.09.004

Cited by 41 publications

(31 citation statements)

References 20 publications

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“…), whilst others found variable cytotoxic effects of different MTA‐based commercial materials (Lee et al . ). Difference in the cell line used, in the protocols for material preparation and setting, in the ratio of sample surface area and volume of the elution medium, in duration of exposure, as well as in the monitored parameters of cell viability, may account for variable results of the aforementioned studies.…”

Section: Discussionmentioning

confidence: 97%

Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study

et al. 2014

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Section: Discussionmentioning

confidence: 97%

Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study

et al. 2014

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“…2 Thus, an ideal root-end filling material must exhibit the following properties: biocompatibility with periapical tissues, 3,4 low cytotoxicity 5 and solubility, 6 appropriate marginal sealing, 7 bond strength to dentin, 8 and bioactivity. Mineral trioxide aggregate (MTA) has calcium silicate (66.1%), calcium silicate (8.4%), calcium aluminate (2.0%), bismuth oxide (14%), calcium oxide (8.0%), silicon oxide ) and aluminum oxide (1.0%), which was the first material to be formulated specifically as a root-end filling material, interacted with periapical fluids and induced formation of hydroxyapatite, 10 leading to the filling of dentin tubules, closure of gaps at the dentin/material interface, and apical sealing.…”

Section: Introductionmentioning

confidence: 99%

Push-out bond strength of different tricalcium silicate-based filling materials to root dentin

et al. 2018

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Push-out bond strength of different tricalcium silicate-based filling materials to root dentin Abstract: The aim of this study was to evaluate the bond strength of different triccalcium silicate cements to retrograde cavity using a push out test. Thirty maxillary central incisors were shaped using #80 hand files and sectioned transversally. Root slices were obtained from the apical 4 mm after eliminating the apical extremity. The specimens were embedded in acrylic resin and positioned at 45° to the horizontal plane for preparation of root-end cavities with a diamond ultrasonic retrotip. The samples were divided into three groups according to the root-end filling material (n = 10): MTA Angelus, ProRoot MTA and Biodentine. A gutta-percha cone (#80) was tugged-back at the limit between the canal and the root-end cavity. The root-end cavity was filled and the gutta-percha cone was removed after complete setting of the materials. The specimens were placed in an Instron machine with the root-end filling turned downwards. The push-out shaft was inserted in the space previously occupied by the gutta-percha cone and push out testing was performed at a crosshead speed of 1.0 mm/min. There was no statistically significant difference in resistance to push out by the materials tested (p > 0.01). MTA Angelus and ProRoot MTA showed predominantly mixed failure while Biodentine exhibited mixed and cohesive failures. The tricalcium silicate-based root-end filling materials showed similar bond strength retrograde cavity.

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“…The viability of pulp cells increased when exposed to highly diluted Biodentine ® suspensions and decreased in a concentration-dependent manner. Similar effects have already been described for different cell species [26, 31–35] also considering time dependent effects [36]. Apart from biocompatibility, we were interested investigating the influence on the main component of the pulpal extracellular matrix.…”

Section: Discussionmentioning

confidence: 75%

Influence of Biodentine® - A Dentine Substitute - On Collagen Type I Synthesis in Pulp Fibroblasts In Vitro

et al. 2016

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Preserving a patient’s own teeth—even in a difficult situation—is nowadays preferable to surgical intervention and therefore promotes development of suitable dental repair materials. Biodentine®, a mineral trioxide aggregate substitute, has been used to replace dentine in a bioactive and biocompatible manner in both the dental crown and the root. The aim of our study was to evaluate the influence of Biodentine® on pulp fibroblasts in vitro. For this study, one to five Biodentine® discs with a diameter of 5.1mm were incubated in DMEM. To obtain Biodentine® suspensions the media were collected and replaced with fresh medium every 24h for 4 days. Primary pulp cells were isolated from freshly extracted wisdom teeth of 20–23 year old patients and incubated with the Biodentine® suspensions. Proliferation, cell morphology, cell integrity and cell viability were monitored. To evaluate the effect of Biodentine® on collagen type I synthesis, the secretion of the N-terminal domain of pro-collagen type I (P1NP) and the release of transforming growth factor-β1 (TGF-β1) were quantified. None of the Biodentine® suspensions tested influenced cell morphology, proliferation or cell integrity. The cell viability varied slightly depending on the suspension used. However, the concentrations of P1NP of all pulp fibroblast cultures treated for 24h with the moderate to high Biodentine® concentration containing suspensions of day 1 were reduced to 5% of the control. Furthermore, a significant TGF-β1 reduction was observed after treatment with these suspensions. It could be shown that Biodentine® is biocompatible. However, dissolved particles of the moderate to high concentrated Biodentine® suspensions 24h after mixing induce a significant reduction of TGF-β1 release and reduce the secretion of collagen type I of primary pulp fibroblasts.

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Cytotoxicity of Newly Developed Ortho MTA Root-end Filling Materials

Cited by 41 publications

References 20 publications

Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study

Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study

Push-out bond strength of different tricalcium silicate-based filling materials to root dentin

Influence of Biodentine® - A Dentine Substitute - On Collagen Type I Synthesis in Pulp Fibroblasts In Vitro

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