Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: <i>in vitro</i> and <i>in vivo</i> study

Petrović, Vera; Opacic-Galic, Vanja; Živković, Slavoljub; Nikolić, Biljana; Danilović, Vesna; Miletić, Vesna; Jokanovic, Vukoman; Mitić-Ćulafić, Dragana

doi:10.1111/iej.12391

Cited by 20 publications

(20 citation statements)

References 29 publications

(62 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These authors studied the effect of MTA in human gingival fibroblasts using flow cytometry and observed that MTA in a diluted medium did not increase apoptosis or necrosis. In contrast, Petrovic et al [40] found that MTA-Angelus was cytotoxic using 50% diluted medium in an MRC5 cell line consisting of human lung fibroblasts. In our experiments, we observed maximal apoptosis for mediums prepared with AH-Plus at 24 h and MTA-Fillapex at 48 h. Regarding AH-Plus, some studies have shown that this endodontic material increased cytotoxicity at 24 h [41, 42], probably due to the presence of amines in its composition [43].…”

Section: Discussionmentioning

confidence: 95%

Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials

Victoria-Escandell

Ibáñez-Cabellos

Cutanda

et al. 2017

Stem Cells International

View full text Add to dashboard Cite

Human dental pulp stem cells (HDPSCs) are of special relevance in future regenerative dental therapies. Characterizing cytotoxicity and genotoxicity produced by endodontic materials is required to evaluate the potential for regeneration of injured tissues in future strategies combining regenerative and root canal therapies. This study explores the cytotoxicity and genotoxicity mediated by oxidative stress of three endodontic materials that are widely used on HDPSCs: a mineral trioxide aggregate (MTA-Angelus white), an epoxy resin sealant (AH-Plus cement), and an MTA-based cement sealer (MTA-Fillapex). Cell viability and cell death rate were assessed by flow cytometry. Oxidative stress was measured by OxyBlot. Levels of antioxidant enzymes were evaluated by Western blot. Genotoxicity was studied by quantifying the expression levels of DNA damage sensors such as ATM and RAD53 genes and DNA damage repair sensors such as RAD51 and PARP-1. Results indicate that AH-Plus increased apoptosis, oxidative stress, and genotoxicity markers in HDPSCs. MTA-Fillapex was the most cytotoxic oxidative stress inductor and genotoxic material for HDPSCs at longer times in preincubated cell culture medium, and MTA-Angelus was less cytotoxic and genotoxic than AH-Plus and MTA-Fillapex at all times assayed.

show abstract

Section: Discussionmentioning

confidence: 95%

Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials

Victoria-Escandell

Ibáñez-Cabellos

Cutanda

et al. 2017

Stem Cells International

View full text Add to dashboard Cite

show abstract

“…) and the study of subcutaneous implantation in rats and rabbits revealed high tolerance to the implanted material (Petrović et al . , Opačić‐Galić et al . ).…”

Section: Introductionmentioning

confidence: 97%

“…CS cements have already been investigated in many in vitro and in vivo studies: MTT assays have revealed increased metabolic activity of L929 and MRC‐5 cells (Petrović et al . , Cetenovic et al . ), bone marrow mesenchymal stem cells showed good proliferation after seeding on this material (Antonijevic et al .…”

Section: Introductionmentioning

confidence: 99%

Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis

et al. 2019

Self Cite

View full text Add to dashboard Cite

Aim To examine the potential systemic toxicity of nanostructured materials based on calcium silicate and calcium aluminate, for potential application in Dentistry. Methodology Twenty‐four Albino Wistar rats aged 2 months were used as an in vivo animal model for subcutaneous implantation of the investigated materials, placed in polyethylene tubes. Thirty days after implantation, the livers of the rats were analysed and following histological and stereological parameters were evaluated for volume density of hepatocytes and blood sinusoids, number and numerical density of hepatocytes, surface of hepatocytes and their nucleuses, nucleocytoplasmic ratio and mitotic index of hepatocytes. Stereological measurements were achieved using Cavalieri's principle, with grid P2 and unbiased analysis. Additionally, immunohistochemistry studies were performed to further analyse changes in liver tissue. Several haematological and biochemical parameters of blood of experimental animals were also analysed, as well as local tissue reactions around the implants. Statistical analysis was performed using parametric (anova and t‐test) and nonparametric tests (Kruskal–Wallis and Mann–Whitney U‐test) depending on data distribution. Results Implanted dental cements led to an increase in stereological and histological parameters in liver tissue compared to control rats. Although the investigated parameters mostly showed significant differences between control and experimental animals, the liver tissue of the experimental animals did not have visible signs of pathological changes. This was supported by the analysis of blood parameters which were not significantly different between control and experimental animals. Also, the subcutaneous tissues had minimal inflammatory reactions. Immunohistochemistry studies revealed that nanostructured materials induced proliferation of hepatocytes, but that the immunological response to the materials was not strong enough to induce proliferation of immunoreactive cells in liver in the observed time period. Conclusions This study was performed as a contribution to the attestation of the biocompatibility of dental cements based on calcium silicate and calcium aluminate. Although these materials induced several changes in the liver structure, they were not clinically relevant and represent a normal and reversible response of the liver to the presence of biocompatible materials in the body. Blood and immunohistochemistry analyses and local tissue reactions further confirmed that these materials possess good biocompatible potential.

show abstract

“…e biocompatibility of ALBO-MPSA has been thoroughly studied and confirmed at in vitro and in vivo conditions and compared to MTA [14][15][16][17][18][19][20][21]. e aim of this paper was to further examine ALBO-MPSA biocompatibility, over the subchronic toxicity study.…”

Section: Introductionmentioning

confidence: 99%

Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates

Jokanovic

Čolović

Prokić

et al. 2018

Advances in Materials Science and Engineering

View full text Add to dashboard Cite

As an alternative to MTA, a new endodontic material based on hydroxyapatite and calcium silicates (ALBO-MPSA) has been synthesized, and its biocompatibility has been studied in manyin vitroandin vivostudies. The current study aims to evaluate a subchronic systemic toxicity of ALBO-MPSA on the rat animal model, as a continuation of the previous studies. Biochemical parameters of blood and histological parameters of the liver, kidneys, and spleen of the rats were analyzed after 120 days of consumption of the aqueous extract of ALBO-MPSA. The results showed no myelotoxic effect or autoimmune effect on peripheral blood cells and no pathological effect on the liver, kidney, and spleen tissues. Besides, no changes in the skin and hair of the rats, neither the change in the consumption of food and water, nor the change in their usual behavior were noticed during the experiment.

show abstract

Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study

Abstract: Evaluation of cytotoxicity and inflammatory response indicated better biocompatibility of CS and HA-CS, in comparison with MTA (White MTA, Angelus(®) Soluções Odontológicas, Londrina, Brazil).

Cited by 20 publications

References 29 publications

Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials

Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials

Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis

Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates

Contact Info

Product

Resources

About