Influence of Biodentine® - A Dentine Substitute - On Collagen Type I Synthesis in Pulp Fibroblasts In Vitro

Abstract

Preserving a patient’s own teeth—even in a difficult situation—is nowadays preferable to surgical intervention and therefore promotes development of suitable dental repair materials. Biodentine®, a mineral trioxide aggregate substitute, has been used to replace dentine in a bioactive and biocompatible manner in both the dental crown and the root. The aim of our study was to evaluate the influence of Biodentine® on pulp fibroblasts in vitro. For this study, one to five Biodentine® discs with a diameter of 5.1mm were incubated in DMEM. To obtain Biodentine® suspensions the media were collected and replaced with fresh medium every 24h for 4 days. Primary pulp cells were isolated from freshly extracted wisdom teeth of 20–23 year old patients and incubated with the Biodentine® suspensions. Proliferation, cell morphology, cell integrity and cell viability were monitored. To evaluate the effect of Biodentine® on collagen type I synthesis, the secretion of the N-terminal domain of pro-collagen type I (P1NP) and the release of transforming growth factor-β1 (TGF-β1) were quantified. None of the Biodentine® suspensions tested influenced cell morphology, proliferation or cell integrity. The cell viability varied slightly depending on the suspension used. However, the concentrations of P1NP of all pulp fibroblast cultures treated for 24h with the moderate to high Biodentine® concentration containing suspensions of day 1 were reduced to 5% of the control. Furthermore, a significant TGF-β1 reduction was observed after treatment with these suspensions. It could be shown that Biodentine® is biocompatible. However, dissolved particles of the moderate to high concentrated Biodentine® suspensions 24h after mixing induce a significant reduction of TGF-β1 release and reduce the secretion of collagen type I of primary pulp fibroblasts.

Biocompatibility and Surface Characteristics of Resin-Modified Glass Ionomer Cements with Ammonium Quaternary Compounds or Silver Nanoparticles: AnIn VitroStudy

“…In part, this is explained because all materials used in the study, both glass ionomers and antimicrobials, have a reported degree of toxicity [48,49]. Different in vitro studies assessed the cytotoxicity of GICs and RMGICs on cultured cells Journal of Nanomaterials [42][43][44][45][46][47][48][49][50][51][52]. The studies have supported the concept that leachable components of the dental materials are responsible for the cytotoxicity in cell culture.…”

“…The in vitro studies can be conducted under controlled conditions and provide a significant amount of information [38]. Different cell lines are used to probe the biocompatibility of dental materials like pulp cells extracted from the maxillary incisors of Sprague Dawley rats, multipotent stromal cells, human foreskin fibroblast, human lung fibroblast, primary human osteoblast, L929 mouse fibroblast, SV40 large T-antigen-transfected bovine dental pulpderived cells, MDPC-23 odontoblast-like cell line, primary pulp fibroblast, and human alveolar osteoblast, for example [39][40][41][42][43][44][45][46]. In this study, the MA-104 epithelial cell line was used because the glass ionomer cements are in direct contact with the epithelial tissue of the oral mucosa.…”

Tooth Repair and Regeneration

“…As demonstrated by Loison-Robert and colleagues [ 28 ], Biodentine promotes mineralization when in contact with dental pulp stem cells (DPSCs); however, it does not affect DPSC proliferation. Interestingly, the only biological factor that Biodentine has shown to increase is TGF-β1 [ 29 ]; however, Neves and Sharpe [ 30 ••] showed that TGF-β and BMP are not pivotal for reactionary dentine formation but for tubular organization of reactionary dentine secretion. Reactionary dentine secretion is affected, however, by changes in the Wnt pathways.…”

Biodentine® y su uso en dentición temporal. Revisión de la literatura

“…Investigaciones odontológicas recientes dentro de un enfoque integral consideran, cada vez más, los dientes y la salud oral como parte de la salud del organismo 1 . Ya no sólo es el dentista el que intenta educar, sino que la población, que engloba tanto a los pacientes como a profesionales de otras áreas de la salud, está mostrando un interés cada vez mayor por la conservación dental.…”

“…del fraguado y un polímero hidrosoluble que actúa como agente reductor de agua 1,2,[5][6][7][8][9][10][11] .…”