Cytotoxicity evaluation of dentin contacting materials with dentin barrier test device using erbium-doped yttrium, aluminum, and garnet laser-treated dentin

Abstract

<jats:sec><jats:title>Objectives:</jats:title><jats:p> The effect of dentin contacting materials on three-dimensional cultures of pulp-derived cells was evaluated in a dentin barrier test device using erbium-doped yttrium, aluminum, and garnet (Er:YAG) laser-treated dentin. </jats:p></jats:sec><jats:sec><jats:title>Methods:</jats:title><jats:p> The test materials (iBond<jats:sup>®</jats:sup>, G-Bond™, and Vitrebond™) were applied on laser-treated or untreated dentin discs. After 24 h of exposure with perfusion of the test chamber, cell survival was evaluated by enzyme activity and related to a nontoxic control material. The mean values of control tissues were set to represent 100% viability. Data were analyzed using Kruskal–Wallis and Mann–Whitney U test. </jats:p></jats:sec><jats:sec><jats:title>Results:</jats:title><jats:p> Vitrebond was the most toxic material for both laser-treated and untreated dentin. On untreated dentin, G-bond was cytotoxic to the pulp-derived cells ( p &lt; 0.05), and iBond was similar to the negative control group ( p &gt; 0.05). However, G-Bond and iBond were not cytotoxic when they were applied to Er:YAG laser-treated dentin ( p &gt; 0.05). </jats:p></jats:sec><jats:sec><jats:title>Conclusion:</jats:title><jats:p> Er:YAG laser treatment of dentin may protect the pulp cells from toxic substances of dentin contacting restorative materials; however, this effect is material related. Taking into consideration the limitations of this in vitro study, the Er:YAG laser treatment of dentin before restoration might be an option for decreasing the cytotoxic effects of the dental materials. Further research is required for clinical applications. </jats:p></jats:sec>