In vitro dentin barrier cytotoxicity testing of some dental restorative materials

Jiang, Ruodan; Lin, Hong; Zheng, Gang; Zhang, X.M.; Du, Qianqian; Yang, Ming

doi:10.1016/j.jdent.2017.01.003

Cited by 43 publications

(52 citation statements)

References 42 publications

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“…Schmalz et al 28 suggested that dentin barrier testing can be used, at least partially, as an alternative to animal experiments. In addition, the cytotoxicity of dental adhesives has been reported to decline with increasing dentin thickness, 29 and vice versa. In the current study, it was also found that the cytotoxic effects vary according to the testing method.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic effects of different self-adhesive resin cements: Cell viability and induction of apoptosis

et al. 2020

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PURPOSE. The effects of four different self-adhesive resin cement materials on cell viability and apoptosis after direct and indirect exposure were evaluated using different cell culture techniques. MATERIALS AND METHODS. Self-adhesive cements were applied to NIH/3T3 mouse fibroblasts by the extract test method, cell culture inserts, and dentin barrier test method. After exposure periods of 24 h and 72 h, the cytotoxicity of these self-adhesive materials was evaluated using the MTT assay (viability) and the Annexin-V-FITC/PI staining (apoptosis). RESULTS. The lowest cell viability was found in cells exposed to BeautiCem SA for 24 h in the extract test method. Cell viability was reduced to 70.6% compared to negative controls. After the 72 h exposure period, viability rate of cell cultures exposed to BeautiCem SA decreased more than 2-fold (29.5%) while cells exposed to RelyX U200 showed the highest viability rate of 71.4%. In the dentin barrier test method, BeautiCem SA induced the highest number of cells in apoptosis after a 24 h exposure (4.1%). Panavia SA Cement Plus was the material that caused the lowest number of cells in apoptosis (1.5%). CONCLUSION. The used self-adhesive cements have showed different cytotoxic effects based on the evaluation method. As exposure time increased, the materials showed more cytotoxic and apoptotic effects. BeautiCem SA caused significantly more severe cytotoxic and apoptotic effects than other cements tested. Moreover, cements other than BeautiCem SA have caused necrotic cell death rather than apoptotic cell death.

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Section: Discussionmentioning

confidence: 99%

Cytotoxic effects of different self-adhesive resin cements: Cell viability and induction of apoptosis

et al. 2020

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“…Studies suggest that because of its low molecular weight and its relative hydrophilic property, HEMA can diffuse through the dentin and reach the pulp, thus affecting odontoblasts viability and, in consequence, altering pulp physiology (21). At the clinical practice, unreacted monomers can penetrate the pulp chamber and reach the pulp cells by diffusion through dentin (11,19) Bonding agents were found to release camphoroquinone, a p h o t o i n i t i a t o r a n d photosensitizer widely used to generate free radicals including reactive oxygen. It has been documented that camphoroquinone acts not only as a cytotoxic agent, but also as a mutagen, and its lixiviation may partly explain why these resinous products are considered toxic agents (27).…”

Section: Discussionmentioning

confidence: 99%

“…Besides the oxygen-inhibited layer preventing complete polymerization, free radicals can react with themselves or other radicals resulting in inactive products (28) and the unreacted monomers can reach the pulp tissue (29,30). Literature reports that monomers such as HEMA are soluble in aqueous medium (31) and are present in adhesive systems such as Single Bond (11). However, under the conditions of the present study, HEMA did not alter the cell viability or metabolism even in direct contact with human dental pulp fibroblasts at 1 nM, in contrast with other works that report decrease of fibroblast grow in HEMA treatment groups (5 mmol/L and and 3 mmol/L) compared to control group (32).…”

Section: Discussionmentioning

confidence: 99%

“…HEMA (H) (2-hydroxy-ethyl-methacrylate) and Single Bond (SB) compound by HEMA, bis-GMA, ethyl alcohol, MDP, silanized silica, water, camphorquinone (3M ESPE, St. Paul, MN, USA) were tested in this study (11). Previously to cell stimulation, both materials were diluted in 10% FBS DMEM.…”

Section: Dental Materials Preparationmentioning

confidence: 99%

“…However, during the inflammatory process CXCL12 expression may be decreased (10). In the case of dental pulps affected by inflammation, some studies have reported increased expression of this chemokine and its receptor (9,11,12). A discrete increase was observed in the production of CXCL12 in human dental pulp stimulated by heat-inactivated Enterococcus faecalis (13).…”

Section: Introductionmentioning

confidence: 99%

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Dental Pulp Fibroblasts Response after Stimulation with HEMA and Adhesive System

et al. 2018

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This study evaluated in vitro cell viability and metabolism, nitric oxide release and production of chemokines by cultured human dental pulp fibroblasts (DPF) under contact with HEMA and Single Bond. Cultures of DPF were established by means of an explant technique. Once plated, cells were kept under contact with increasing concentrations of HEMA (10, 100 and 1000 nM) or Single Bond (SB) [10-fold serially diluted in culture medium (10 -4 , 10 -3 and 10 -2 v/v)] and also with polymerized SB components. Cytotoxicity was assessed by Trypan Blue exclusion method and MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Nitric oxide release on cell supernatant was detected by Griess Method whereas chemokines (CXCL12 and CXCL8) were detected by ELISA. RT-qPCR was employed for chemokines gene expression analysis. Cytotoxic tests showed significant differences for SB 10 -2 . None of the tested materials significantly altered NO levels. Protein levels of CXCL12 were significantly decreased only by HEMA. On the other hand, while CXCL12 mRNA remained unaltered, gene expression of CXCL8 had significant decrease with all materials, except for polymerized SB. In conclusion, Single Bond and HEMA at various concentrations, decreased expression and production of molecules involved in inflammatory processes and, therefore, the use of adhesive systems such as pulp capping materials must be viewed with caution due to its large cytotoxic effect when in close contact with the pulp.

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Impact of bisphenol A and analogues eluted from resin‐based dental materials on cellular and molecular processes: An insight on underlying toxicity mechanisms

Rajkumar,

Padmanaban

2024

J of Applied Toxicology

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Dental resin systems, used for artificial replacement of teeth and their surrounding structures, have gained popularity due to the Food and Drug Administration's (FDA) recommendation to reduce dental amalgam use in high‐risk populations and medical circumstances. Bisphenol A (BPA), an endocrine‐disrupting chemical, is an essential monomer within dental resin in the form of various analogues and derivatives. Leaching of monomers from resins results in toxicity, affecting hormone metabolism and causing long‐term health risks. Understanding cellular‐level toxicity profiles of bisphenol derivatives is crucial for conducting toxicity studies in in vivo models. This review provides insights into the unique expression patterns of BPA and its analogues among different cell types and their underlying toxicity mechanisms. Lack of a consistent cell line for toxic effects necessitates exploring various cell lines. Among the individual monomers, BisGMA was found to be the most toxic; however, BisDMA and BADGE generates BPA endogenously and found to elicit severe adverse reactions. In correlating in vitro data with in vivo findings, further research is necessary to classify the elutes as human carcinogens or xenoestrogens. Though the basic mechanisms underlying toxicity were believed to be the production of intracellular reactive oxygen species and a corresponding decline in glutathione levels, several underlying mechanisms were identified to stimulate cellular responses at low concentrations. The review calls for further research to assess the synergistic interactions of co‐monomers and other components in dental resins. The review emphasizes the clinical relevance of these findings, highlighting the necessity for safer dental materials and underscoring the potential health risks associated with current dental resin systems.

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In vitro dentin barrier cytotoxicity testing of some dental restorative materials

Cited by 43 publications

References 42 publications

Cytotoxic effects of different self-adhesive resin cements: Cell viability and induction of apoptosis

Cytotoxic effects of different self-adhesive resin cements: Cell viability and induction of apoptosis

Dental Pulp Fibroblasts Response after Stimulation with HEMA and Adhesive System

Impact of bisphenol A and analogues eluted from resin‐based dental materials on cellular and molecular processes: An insight on underlying toxicity mechanisms

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