Cytotoxic triterpenoid saponins from the root of Anemone tomentosa (Maxim.) Pei

Abstract

Two new triterpenoid saponins (1-2), with eleven known compounds (3-13) were obtained from ethyl acetate extracts of Anemone tomentosa (Maxim.) Pei by bioactivity-guided isolation. Their structures were elucidated by chemical and spectral analysis, including 1D, 2D NMR data and HRESIMS. The cytotoxic activity of all isolated saponins was evaluated on MDA-MB-231 and PC-3 cell lines.Compounds 5 and 7 showed significant cytotoxicity against with MDA-MB-231 cell and PC-3 cell with IC 50 values of 3. 73-4.21 μM and 3.53-5.37 μM, respectively.

Current perspectives on naturally occurring saponins as anticancer agents

“…Pei, and 3‐ O ‐β‐ d ‐ribopyranosyl‐(1→3)‐α‐ʟ‐rhamnopyranosyl‐(1→2)‐β‐ d ‐xylopyranosyl oleanane acid ( 32 , IC 50 : 3.73 and 4.21 µM, MTT assay) was found to be most active against PC‐3 and MDA‐MB‐231 cells, and the activity was not inferior to that of cisplatin (IC 50 : 2.20 and 6.12 µM). [ 48 ] Arenaroside D ( 33 , IC 50 : 6.1–9.4 µM, MTT assay), a polyhydroxylated oleanane‐type saponin from Polycarpaea arenaria , demonstrated promising activity against A549, HTC116, PC3, and RT112 cancer cell lines. [ 49 ] Accordingly, these triterpenoid saponins were potential anticancer candidates and merited further evaluation.…”