Cytotoxic Triterpenoid Saponins from <i>Lysimachia clethroides</i>

Liang, Dong; Hao, Zhi‐You; Zhang, Guijie; Zhang, Qingjian; Chen, Ruo-Yun; Yu, De‐Quan

doi:10.1021/np2004038

Cited by 53 publications

(28 citation statements)

References 13 publications

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“…In this paper, we first report the isolation and structure elucidation of these compounds. [7] and xylitol [8] by GC analysis. In the HMBC spectrum ( Figure 2 Journal of Asian Natural Products Research 931 (Figure 2(b)).…”

Section: Introductionmentioning

confidence: 99%

Two new compounds from the dry leaves of Pleioblastus amarus (Keng) keng f.

Sun

Tang

Yue

et al. 2014

Journal of Asian Natural Products Research

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Two new compounds, xylitol 1-O-(6'-O-p-hydroxylbenzoyl)-glucopyranoside (1) and bambulignan B (2), together with three known ones gastrodin (3), glucovanillin (4), and rel-(7S,7'R,8R,8'S)-4,4'-dihydroxy-3,3',5,5'-tetramethoxy-7,7'-epoxyligna-9,9'-diol-9(or)9'-O-β-glucopyranoside (5), were isolated from the 95% EtOH extract of the dry leaves of Pleioblastus amarus (Keng) keng f. Their structures were determined by UV, IR, HR-ESI-MS, CD, and 1D and 2D NMR data analyses as well as GC experiments.

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Section: Introductionmentioning

confidence: 99%

Two new compounds from the dry leaves of Pleioblastus amarus (Keng) keng f.

Sun

Tang

Yue

et al. 2014

Journal of Asian Natural Products Research

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“…Triterpenoid saponins, which are structurally defined as the glycosides of triterpenes, are one of the most widely distributed components with a large diversity of chemical structures and biological activities, such as antimicrobial, antiviral, and antifungal activities [5][6][7]. It has also been well demonstrated that triterpenoid saponins can potently suppress the proliferation of various human cancer cell lines, including lung [8][9][10], stomach [8,9], colon [9,10], breast [11], and liver cancer [9][10][11]. In a recent study, we further showed that triterpenoid saponins with a 13,28epoxy moiety in the oleanane-type sapogenin structure exerts a tumor selective cytotoxic action toward human liver cancer cells without affecting the viability of normal liver cells [12].…”

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confidence: 99%

Cyclamin, a Natural 13,28-Epoxy Triterpenoid Saponin, Synergistically Enhances the Cytotoxicity of Chemotherapeutic Drugs in Human Liver Cancer Cells but Not Non-Neoplastic Liver Cells

Deng

et al. 2014

Planta Med

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In this study, a diverse set of triterpenoid saponins was evaluated to assess their chemosensitizing activity in two human liver cancer cells (Bel-7402 and HepG2) and non-neoplastic HL-7702 liver cells. Cyclamin, a 13,28-epoxy oleanane-type triterpenoid saponin from Ardisia japonica, was identified as a potent chemosensitizer. A low cytotoxic level of cyclamin synergistically enhances the growth inhibitory effect of 5-fluorouracil (from 41.3 ± 1.1% to 64.5 ± 2.3%), cisplatin (from 57.1 ± 1.5% to 79.6 ± 2.7%), and epirubicin (from 62.6 ± 1.2% to 74.9 ± 1.8%) on Bel-7402 cells, but not HL-7702 cells. Flow cytometric analysis shows that cyclamin synergistically enhances the apoptosis and cell cycle arrest induction effects of 5-fluorouracil by 69.4% and 22.2%, respectively. The mechanism of action could be through the activation of caspase-3, -8, and -9, the upregulation of cyclin-dependent kinase 2, the cell division cycle 25 homolog A expression level, or the Bax/B-cell lymphoma 2 ratio. Furthermore, a lactate dehydrogenase release assay demonstrated that cyclamin markedly increases the membrane permeability of Bel-7402 cells, which may also contribute to the mechanism of the chemosensitizing activity of cyclamin. These findings indicate the potential usefulness of cyclamin for the chemoprevention and treatment of liver cancer.

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“…All the cells were cultured in DMEM medium, supplemented with 10% fetal bovine serum. The cytotoxic assay was performed according to the MTT method (Liang et al, 2011). Briefly, 180 mL of adherent cells were seeded into each well of 96-well cell culture plates and allowed to adhere for 12 h before drug addition, while suspended cells were seeded just before drug addition, with an initial density of 5 Â 10 4 cells/well.…”

Section: Assay For Inhibition Of Human Liver Cancermentioning

confidence: 99%