Six new quassinoids (1-6) and eight known compounds of this type (7-14) were isolated from the seeds of Brucea javanica. Their structures were elucidated by analysis of their spectroscopic data and from chemical evidence. Compounds 1-5 were found to be unusual quassinoids with a 2,3-seco A ring. The configurations at C-4 in 4 and 5 were determined by a difference circular dichroism method. In in vitro bioassays, 8 and 10 showed inhibitory activities for nitric oxide production in LPS-activated macrophages, with IC(50) values of 1.9 and 5.0 μM, respectively, while compounds 6, 8-11, 13, and 14 exhibited cytotoxicity against five human tumor cell lines (HCT-8, HepG2, BGC-823, A549, and SKVO3), having IC(50) values in the range 0.12-9.3 μM.
Capacity uncertainty is a common issue in the transportation planning field. However, few studies discuss the intermodal routing problem with service capacity uncertainty. Based on our previous study on the intermodal routing under deterministic capacity consideration, we systematically explore how service capacity uncertainty influences the intermodal routing decision. First of all, we adopt trapezoidal fuzzy numbers to describe the uncertain information of the service capacity, and further transform the deterministic capacity constraint into a fuzzy chance constraint based on fuzzy credibility measure. We then integrate such fuzzy chance constraint into the mixed-integer linear programming (MILP) model proposed in our previous study to develop a fuzzy chance-constrained programming model. To enable the improved model to be effectively programmed in the standard mathematical programming software and solved by exact solution algorithms, a crisp equivalent linear reformulation of the fuzzy chance constraint is generated. Finally, we modify the empirical case presented in our previous study by replacing the deterministic service capacities with trapezoidal fuzzy ones. Using the modified empirical case, we utilize sensitivity analysis and fuzzy simulation to analyze the influence of service capacity uncertainty on the intermodal routing decision, and summarize some interesting insights that are helpful for decision makers.
Seven oleanane-type triterpenoid saponins, named clethroidosides A-G (1-7), an ursane-type triterpenoid saponin, clethroidoside H (8), and six known saponins were isolated from the aerial parts of Lysimachia clethroides. The structures of the saponins were elucidated on the basis of physical data analysis (1D and 2D NMR, HR-ESIMS) and chemical evidence. The cytotoxic activities of compounds 1-14 were evaluated against five human tumor cell lines (HT-29, HePG2, BGC-823, A549, and A375). Compounds 3, 4, 6, and 11-13 exhibited moderate cytotoxic activity, with IC50 values of 0.75-2.62 μM, while compound 5 showed selective cytotoxic activity.
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