Cytotoxic and nitric oxide inhibitory activities of methanol extracts of<i>Garcinia</i>species

Jabit, Md. Lip; Wahyuni, Fatma Sri; Khalid, Rozida Mohd.; Israf, Daud Ahmad; Shaari, Khozirah; Lajis, Nordin Hj; Stanslas, Johnson

doi:10.3109/13880200902973787

Cited by 37 publications

(18 citation statements)

References 26 publications

(26 reference statements)

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“…were evaluated for their cytotoxicity and anti-inflammatory activity using MTT and Griess assay method, respectively. 3 Ethanol extract of the stem bark of G. cowa also active on T47D breast cancer cell. Several compounds, [2E ,6E ,10E ]-(+)-4b -hydroxy-3-methyl-5b -(3,7,11,15-tetramethyl-2,6,10,14-hexadecatetraenyl-2-cyclohexen-1-one (1), 6-hydroxycalabaxanthone (2), 2-(3-methyl-2-butenyl)-1,5,6-trihydroxy-3-methoxy-4-(1,1-dimethyl-2-propenyl)-9H-xanthen-Cite this article : Wahyuni FS, Ali DAI, Lajis NH, Dachriyanus.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory activity of isolated compounds from the stem bark of Garcinia cowa Roxb

Wahyuni¹,

Ali²,

Lajis³

et al. 2016

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Objective: To find the anti inflammatory active compounds from methanol extract of Garcinia cowa Methods: To evaluate the inhibitory activity of isolated compounds on nitric oxide (NO) production, culture media was assayed using Griess reaction. An equal volume of Griess reagent (1% sulphanilamide and 0.1% N-(L-naphthyl)-ethylene diamine dihydrochloride, dissolved in 2.5% H3PO4) was mixed with culture supernatant and color development was measured at 550 nm using a micro plate reader. The amount of nitrite in the culture supernatant was calculated from a standard curve (0-100 μM) of sodium nitrite freshly prepared in deionized water. Percentage of the NO inhibition was calculated by using nitrate level of IFN-γ/LPS-induced group as the control. Results: Isolated compounds, tetraprenyltoluquinone, rubraxanthone and α-mangostin from stem bark of Garcinia cowa Roxb were evaluated for their anti-inflammatory activity. Only α-mangostin exhibited strong anti-inflammatory activity with 83.42 % inhibition of NO and without inducing severe cytotoxicity at 50 μM. Rubraxanthone showed weak inhibition of NO with 23.86 % inhibition of NO while maintained 77.32 % of cell viability. TPTQalso showed the strong inhibition of NO with 80.98 % inhibition but unfortunately this compound also induced severe cytotoxicity with 39.62% viability. Conclusion: α-Mangostin exhibited strong anti-inflammatory activity without inducing severe cytotoxicity at 50 μM. Rubraxanthone showed weak inhibition of NO while Tetraprenyltoluquinone also showed the strong inhibition of NO however this compound also induced severe cytotoxicity.

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Section: Introductionmentioning

confidence: 99%

Anti-inflammatory activity of isolated compounds from the stem bark of Garcinia cowa Roxb

Wahyuni¹,

Ali²,

Lajis³

et al. 2016

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“…This genus is reported to contain quinone, 3 xanthones, 3,4 benzophenones, 5 triterpenes, 6 biflavonoids 7 and benzoquinone. 8 The pharmacological activities of this genus were anticancer, 9,10 anti-inflammatory, 11 antibacterial, 12 antiviral, 13 antifungal and antioxidants.…”

Section: Introductionmentioning

confidence: 99%

Sub-acute Toxicity Study of The Ethyl Acetate Fraction of Asam Kandis Rinds (Garcinia cowa Roxb.) on the Liver and Renal Function in Mice

Wahyuni¹,

Arisanty²,

Hayaty³

et al. 2017

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Objective: The present study investigated the sub acute toxicity of the ethyl acetate fraction of asam kandis (Garcinia cowa Roxb) Rinds in mice. Material and Methods: Sub acute toxicity study was carried out by giving orally at dose 500, 1000 dan 2000 mg / kgBW extract to five mice at 21 days. Animals were observed individually for any clinical signs of toxicity or mortality for 14 days. Measured parameters were SGPT levels, serum creatinine levels, weight ratio of liver and kidney. Extract was given orally at dose 500, 1000 and 2000 mg/kgBW for 21 days. Observations were done on day 8th, 15th and 22th using blood serum, liver and kidneys of mice. Data were analyzed by using two-way ANOVA followed by Duncan's Multiple Range Test. Results: The ethyl acetate fraction of G. cowa at doses 500, 1000 and 2000 mg/kgBW gave significant effect on increasing SGPT levels and decreasing levels of serum creatinine (p <0.05). The length of treatment gave significant effect on decreasing levels of serum creatinine, weight ratio of liver and kidney (p <0.05). Conclusion: The dosage of the ethyl acetate fraction of asam kandis rinds provides significant effect on the SGPT and serum creatinine levels of male white mice. The duration of administration of ethyl acetate fraction of asam kandis rinds provides significant effect on serum creatinine levels, the weight ratio of liver and kidney organ of male white mice.Key words: Sub-acute toxicity, Garcinia cowa rinds, SGPT, Creatinine serum, Weight ratio of liver and kidney. Liver disorders characterized by elevated serum transaminase activity such SGPT (Serum Glutamic Piruvic Transaminases) in serum. 21 Kidney also the main target organ of the toxic effect, because its produce urine which is the main route of excretion toxicant and has a high volume of blood flow. One indicator of kidney damage is an increase or a decrease in creatinine levels in the body. Cite this 22 MATERIALS AND METHODS Chemicals and ReagentsAssay kits for kidney and liver function indices were products of Randox Laboratories limited, United Kingdom. Other chemicals and reagents were all of analytical grade. Plant Collection, Authentication and ExtractionFresh rinds of Garcinia cowa were collected from Batu Busuk, Padang, West Sumatra, Indonesia in August, 2015. The plant was identified and authenticated at the Herbarium Andalas University (ANDA) and was assigned a voucher number FSW-001 after which a voucher specimen was prepared and deposited at the University Herbarium. Fresh Rinds of G. cowa were then chopped into small pieces, air-dried at room temperature for 10 days to a constant weight and subsequently pulverized into fine powder. Powder sample (500 g) was soaked in 4 liters of 70% ethanol for 24 hours. The extract was filtered (with Whatman No. 1 filter paper) and the resulting filtrate was concentrated with a rotary evaporator (40°C). After that, the product was lyophilized to give 12.0 g of residue, according to a yield of 2.4%. This was then stored in a desiccator for further use. Experiment...

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“…The bark, latex and root have been used as an antipyretic agent, while fruit and leaves have been used as an expectorant, for indigestion and improvement of blood circulation. 1 The extract of this plant was reported to have various biological activity such as cytotoxicity, [2][3][4] antiinflamatory. 2 Rubraxanthone (Figure 1) has been isolated as major component of the stem bark of G. cowa.…”

Section: Introductionmentioning

confidence: 99%

“…1 The extract of this plant was reported to have various biological activity such as cytotoxicity, [2][3][4] antiinflamatory. 2 Rubraxanthone (Figure 1) has been isolated as major component of the stem bark of G. cowa. 5,6,7 This compound has antimicrobial 8 and antioxidant activity.…”

Section: Introductionmentioning

confidence: 99%

Determination of Rubraxanthone in the Latex of Asam Kandis (Garcinia cowa Roxb) by Reverse Phase High Performance Liquid Chromatography

Hamidi¹,

Asjar²,

Susanti³

2017

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Context: Rubraxanthone is a major compound found in Garcinia cowa Roxb which has various biological activities. This compound is likely to be responsible for the pharmacological activities of this plant. The latex of this plant was one of the source of this compound. To prevent counterfeiting, it is essential to develop a method of analysis to determine the levels of these compounds in the latex of G. cowa. Aims: To develop and validated a reverse phase-high performance liquid chromatography (RP-HPLC) technique for determination of rubraxanthone in the latex of G. cowa. Settings and Design: RP-HPLC analysis. Methods and Material: The sample was powdered and dissolve in methanol and then subjected to Reverse Phase High Performace Liquid Chromatoraphy (RP-HPLC). Separation was carried out in a reversed-phase column Shimadzu Shimp-pack VP-ODS (4.6 x 250 mm). The elution was performed with isocratic solvent using formic acid 0.4 % v/v in methanol (15:85) with a flow rate 1 ml/minute. The solvents used for the mobile phase were filtered through membrane filter (0.45 mm pore size) and degassed before use. Total running time was 20 minutes and the sample injection volume of injection was 20 ml. While the wavelength of the UV-VIS detector was set at 243.2 nm. Results: The detection and the quantitation limits of rubraxanthone were 1.119 mg/mL and 3.731 μg/mL, respectively. A regression analysis was performed, with the observation of good linearity (r = 0.998). The values obtained for precision and accuracy determination are in agreement with ICH guidelines. It was found that rubraxanthone in dichloromethane extract of latex G. cowa was 56.56%. Conclusions: The results demonstrated that the developed method is a reliable HPLC technique for determination of rubraxanthone in the latex of G. cowa.

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Cytotoxic and nitric oxide inhibitory activities of methanol extracts ofGarciniaspecies

Cited by 37 publications

References 26 publications

Anti-inflammatory activity of isolated compounds from the stem bark of Garcinia cowa Roxb

Anti-inflammatory activity of isolated compounds from the stem bark of Garcinia cowa Roxb

Sub-acute Toxicity Study of The Ethyl Acetate Fraction of Asam Kandis Rinds (Garcinia cowa Roxb.) on the Liver and Renal Function in Mice

Determination of Rubraxanthone in the Latex of Asam Kandis (Garcinia cowa Roxb) by Reverse Phase High Performance Liquid Chromatography

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