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Rubraxanthone is one of antibacterial and antioxidant compound which is isolated from stem bark of mangosteen (Garcinia mangostana Linn.). The precise method for analysis of plant constituents is normally a reverse-phase high performance liquid chromatographic (RP-HPLC). Development and validation of a RP-HPLC method for chromatographic separation was carried out on a reversed-phase column Shimadzu ® Shimp-pack VP -ODS (4.6x250mm) using an isocratic mobile phase of 0.4% formic acid -acetonitrile at a flow rate 1mL min -1 , and detection was with a UV detector. The linearity of the proposed method was found in the range of 2.5-25μg/mL -1 with regression coefficient 0.999. Intraday precision studies showed the relative standard deviation ≤1.58% and inter-day ≤3.20%. Accuracy of the method was determined by a recovery study conducted at 3 different levels, and the average recovery was 102.18%. The Limit of Detection Limit of Quantitation (LOQ) and were 0.47 and 1.56μg/mL -1 .The contents of rubraxanthon in the crude was 0.23±0.07%w/w.

Telah dilakukan uji perlindungan terhadap sinar UV oleh senyawa hasil fraksinasi ekstrak metanol semipolar kulit buah G. mangostana Linn secara in vitro dengan menggunakan alat spektrofotometer UV-Visibel. Uji perlindungan terhadap sinar UV dilakukan berdasarkan metode Mansur dengan mengukur serapan larutan uji dalam pelarut etanol pada konsentrasi 20 mg/100 ml pada panjang gelombang 290 sampai 320 nm. Hasil pengujian secara in vitro menunjukkan bahwa fraksi n-heksan memiliki kemampuan perlindungan UV sedang ; SPF (Sun Protection Factor) 15,02 ± 0,01, fraksi diklorometan dan  butanol memiliki kemampuan perlindungan UV ultra.dengan SPF masing-masingnya adalah 67,62 ± 1,68 dan 61,12 ± 0,24. Hasil penelitian menunjukkan fraksi diklormetan memiliki potensi proteksi terhadap sinar UV paling baik dibanding kedua fraksi yang lain.tify'  Key word : Garcinia mangostana, tyle='mso-bidi-font-style:normal'urkumin dan analognya, SPRMs, docking, reseptor  progesteron IA28 -language:IN'Antiradikal, DPPH, IC50, fenolik total, Elephantopus schaber L., Ocimum basilicum L.forma citratum Back., Graptophylum pictum Griff, Gynura procumbens Merr. 

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