Cytotoxic Action of Hepatoprotector Antral on Bull Sperm

Abstract

Introduction. According to the European legislation and requirements, industrial production of medication shall be provided with well-defined regulations of the quality of such preparation. Material and methods. Cytotoxic action of hepatoprotector Antral on bull sperm was evaluated. Samples of sperm were divided into control and two experimental samples: control one was diluted with phosphate-buffered saline (PSB), which included NaCl - 0.8 g, KСl - 0.02 g, Na2HPO4 - 0.11 g, КH2PO4 - 0.02 g, MgCl2 - 0.01 г, Н2О till 100ml; in the first experimental sample PSB was added with antral in the dose 1/500 LD50 (2.77 mg); in the second - PSB with Antral in the dose 1/100 LD50 (13.87 mg) was added. The survival of spermatozoa was defined until termination of rectilinear forward movement in sperm, which was preserved in temperature of 2-5 °C; respiratory activity was defined (by polarography (ng-atom O/0.1 ml of semen (S) ∙ min) in thermostated sell (temperature of 38.5 °C), with the volume of 1.0 ml with the automatic registration of process flow by potentiometer, the proportion of decrease of which was evaluated in accordance to impacted dose of antral in substrate and restorative activity was defined potentiometrically (mV/0.1ml C ∙ min) using a system of pen microelectrodes that were inserted in thermostated polarographic cell). Results. In the experimental samples of semen in comparison with the control sample the respiratory and restorative activities were reduced; dose 1/500 LD50 to 58.8% and dose 1/100 LD50 to 68.5%; restorative activity - to 66.7% and 54.5% correspondingly. Conclusion. Antral reduces the survival of sperm being irrespective of dose.

Antibacterial Efficacy of Silver or Arsenic Doped Polymer Composites Against Several Kinds of Bacteria

“…Solubilization of Sudan III was performed using the procedure described by Demchuk et al 33 Survival and activity of sperm oxidative enzymes were performed using the procedure described by several authors. 34,35…”

Одержання Та Дослідження Властивостей Комбінованих Альгінат-Желатинових Гідрогелів

“…To assess the biocompatibility and cytotoxicity of hydrogel matrices (combined hydrogels) a test culture of bull sperm was used [32]. Ejaculates with the following physiological characteristics were selected for studies: volume 3-4 ml, sperm concentration 6-11•10 8 cells/ml, number of live germ cells 80-85 %.…”