2009
DOI: 10.1021/la9000158
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Cytosensing and Evaluation of Cell Surface Glycoprotein Based on a Biocompatible Poly(diallydimethylammonium) Doped Poly(dimethylsiloxane) Film

Abstract: In this paper, we constructed an interface that not only retains viability of immobilized BGC823 human gastric carcinoma cells (BGC823 cells) but also efficiently resists nonspecific adsorption of the P-glycoprotein antibody and its secondary antibody, which enabled us to sensitively detect the number of cells and P-glycoproteins on the BGC823 cell surface by the immunoassay method. Preparation of the film was quite simple and inexpensive just by spin-coating poly(dimethylsiloxane) (PDMS) doped with poly(diall… Show more

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Cited by 21 publications
(16 citation statements)
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“…Recently, living cell detection draws more attention for its close relationship with life science research, toxicity monitoring, clinical diagnostics, and public health protection (Shao et al, 2009). Photoelectrochemistry, as a newly developed analytical method based on the photoelectrochemical properties of the materials, has a promising potential in biological analysis (Haddour et al, 2006;Pardo-Yissar et al, 2003;Wang et al, 2009a,b,c).…”
Section: Introductionmentioning
confidence: 99%
“…Recently, living cell detection draws more attention for its close relationship with life science research, toxicity monitoring, clinical diagnostics, and public health protection (Shao et al, 2009). Photoelectrochemistry, as a newly developed analytical method based on the photoelectrochemical properties of the materials, has a promising potential in biological analysis (Haddour et al, 2006;Pardo-Yissar et al, 2003;Wang et al, 2009a,b,c).…”
Section: Introductionmentioning
confidence: 99%
“…Biofunctionalized NPs with recognizer biomolecules, which combine the specificity of biomolecules with the signal amplifying properties of NPs, can be utilized to obtain highly sensitive electrochemical cytosensing [94]. By evolution of electrochemical cytosensors, a variety of cytosensors and biomaterial interfaces have been designed in order to immobilize cells and evaluate cell surface carbohydrates and glycoproteins, as changes in their expression may signal various diseases, especially cancer [95, 96]. Also, many investigations have been carried out to identify suitable cancer-related receptors and their specific binding recognizers.…”
Section: The Functions Of Mnps In Various Biosensor Typesmentioning
confidence: 99%
“…By virtue of the current magnification of the ECR, a broad linear range with the detection limit as low as 10 cells mL À 1 was obtained even in the presence of a large number of normal cells. In contrast to the commonly used EIS technology, fast-response DPV method prevented immobilized cells from long exposure to an electric field, allowing the detection of living cells (Shao et al, 2009).…”
Section: Electrochemical Cytosensorsmentioning
confidence: 99%