Cytokines Genetic Polymorphism: The Past and the Future

Our aimwas to study the influence of IL4-589C>T gene polymorphism on IL-4 expression in patients, living in Zabaikalsky Region with a diagnosis of chronic traumatic osteomyelitis developing after fractures of long bones of extremities.The study included 132 patients with fractures of long bones at the age of 20 to 40 years old. The first group consisted of 83 patients with uncomplicated course of fractures; the second group (n = 49) included patients with chronic traumatic osteomyelitis healed by primary wound closure, however, developing chronic traumatic osteomyelitis over late postoperative period. The control group consisted of 100 practically healthy men and women of the same age group. These groups were homogeneous and similar by age, sex, origin and localization of fractures. Presence of acute or chronic comorbidities was the exclusion criterion. We applied clinical examination, instrumental tests (X-ray studies), laboratory methods (microbiological, immunological, ELISA for IL-4 measurement). IL-4 point mutation at position 589 (C>T) was tested by PCR; the DNA for this analysis was extracted from peripheral blood. The genetic studies were performed when patients entered the hospital. IL-4 contents, clinical and instrumental indicators were detected on 1, 2, 10 and 90 days after fracture. Resultsof the study were as follows: the frequency of the C allele and the homozygous genotype of the IL4- 589C>T gene in the patients with complicated clinical course was decreased 1.7- and 3.6-fold compared with the comparison clinical group, respectively. Frequency of T allele, by contrast, increased 3.7-fold, whereas heterozygous and mutant genotypes were changed 2.5 and 15 times against the comparison group. The persons from control group with IL4-589C/C genotype, showed a 1.4- and 2.5-fld increased IL-4 concentration as compared with the carriers of the -589C/T and -589T/T genotypes, respectively, and in -589C C/T genotype, 1.7-fold compared to -589Т/T genotype. A similar trend of IL-4 expression, depending on the IL4 genotype, was recorded in the group with both uncomplicated and complicated course of fractures. In conclusion, the patients with developing traumatic osteomyelitis showed ncreased frequency of the IL4 -589T allele (3.7-fold as compared with uncomplicated group). Meanwhile, higher frequency of the -589C/T -589T/T genotypes of the IL4 gene were registered. Genotype -589T/T of the IL4 gene is associated with lower IL-4 expression.

show abstract

IL4-589C>T GENE POLYMORPHISM AND EXPRESSION OF INTERLEUKIN-4 IN PATIENTS WITH DEVELOPING CHRONIC TRAUMATIC OSTEOMYELITIS

Мироманов

Миронова

Miromanova

2018

Med. immunol.

View full text Add to dashboard Cite

show abstract

Frequency of Cytokine Gene Polymorphism in Full-Term Newborns with Hypoxic Events

Panova

Панченко

Pushkarev

2020

Acta biomedica scientifica

View full text Add to dashboard Cite

Background. An infant brain damage is an extremely urgent problem, this pathology is difficult to prevent, and subsequently it manifests itself with a variety of neurological consequences. Various mechanisms are involved in neurodamage; cytokines, as well as genes that control their activity, are under a great concern today. However, there is little data about their role as predictors of the brain damage among children after hypoxia. Aim of the research. To identify the frequency of cytokine gene polymorphism: interleukin (IL)-1β(C-511T), IL-1β(C3953T), IL-4(C589T), IL-6(C174G), IL-10(C819T), IL-10(G1082A) among newborns with hypoxic events. Materials and methods. The study involved 128 full-term newborn patients with hypoxic events: the first group (n = 48) included newborns who experienced chronic intrauterine hypoxia (CVH), the second group (n = 80) included newborns born in asphyxiation. Control group (52) included babies born without asphyxia and not suffering from CVH. A retrospective analysis of case-records was carried out. The material for molecular genetic analysis was DNA samples isolated from umbilical cord blood leukocytes using DNA Express Blood reagents (Scientific and Production Company LITECH, Moscow). Results. Compared to the control group (p = 0.03) children born in asphyxia had their T allele IL-1β (C-511T) prevailed. The group of newborn who had CVH had their TT genotype (p = 0.04) and the T IL-1β allele (C-511T) (p = 0.01) prevailed compared to the control group. In the same study group while studying the polymorphism of the IL-1β gene, the T allele (p = 0.03) at the point C3953T prevailed, in contrast to the control group. Conclusion. Due to the fact that cytokines are part of a reaction cascade leading to the secondary brain damage, under the action of hypoxia, it was found that among newborns undergoing asphyxia and chronic intrauterine hypoxia the increased frequencies of carriage of IL-1β-511TT and IL-1β-3953TT genotypes, and IL-1β-511T and IL-1β-3953T alleles increase the risk of neurodamage.

show abstract

A retrospective analysis of the incidence of chickenpox in the Altai territory and the city of Barnaul for 2001–2019

Peredelskaya

Safyanova

2020

Sanitarnyj Vrač (Sanitary Doctor)

View full text Add to dashboard Cite

Chickenpox is an urgent problem for public health due to its high contagiousness, wide prevalence, high incidence rates, a significant probability of medium and severe clinical course of the disease, leading to death and disability, diseases of pregnant women and the development of congenital forms of infection, as well as the carrier of the Varicella zoster virus, which leads to a chronic form of infection — shingles. Purpose: of this study was to assess epidemiological situation of chickenpox incidence among the population of the Altai region and city of Barnaul in 2001–2019 years. Materials and methods. The materials of the study were the data of statistical reporting forms № 2 of the Federal state statistical observation «Information on infectious and parasitic diseases» in the Altai region and the city of Barnaul for 2001–2019. Data processing was carried out using the calculation of intensive and extensive indicators, the calculation of the arithmetic mean (X) and the standard error of the mean (m). Statistical analysis was carried out using Microsoft Excel. Results. During the study period, there was a tendency to increase the incidence of chickenpox among the population of the Altai region and the city of Barnaul. Winter-spring seasonality was observed. Among the sick in the Altai region and in the city of Barnaul dominated children under 17 years. Summary. In order to prevent drifts and the spread of chickenpox, it is necessary to introduce vaccination against this infection into the national calendar of preventive vaccinations of the Russian Federation.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Cytokines Genetic Polymorphism: The Past and the Future

Cited by 3 publications

References 16 publications

IL4-589C>T GENE POLYMORPHISM AND EXPRESSION OF INTERLEUKIN-4 IN PATIENTS WITH DEVELOPING CHRONIC TRAUMATIC OSTEOMYELITIS

IL4-589C>T GENE POLYMORPHISM AND EXPRESSION OF INTERLEUKIN-4 IN PATIENTS WITH DEVELOPING CHRONIC TRAUMATIC OSTEOMYELITIS

Frequency of Cytokine Gene Polymorphism in Full-Term Newborns with Hypoxic Events

A retrospective analysis of the incidence of chickenpox in the Altai territory and the city of Barnaul for 2001–2019

Contact Info

Product

Resources

About